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The nBAF complex subunit CREST/SS18L1 regulates hippocampal memory processes via tyrosine 397 and histone acetyltransferase CBP.

Overview

Authors: Franklin G Garcia1,2, Maria Farias de Albuquerque1,2, Vanessa Johnson1,2, Enikö Kramár1,2, Kousha Changizi3, Sherif Abdelkarim3, Agatha S Augustynski1,2, Dina P Matheos1,2, Joseph Picone1,2, Tracy L Fetterly1,2, Alyssa Rodriguez1,2, Jessica Childs1,2, Angela Gomez-Arboledas4, Julia Winter5, Elizabeth Heller5, Pierre Baldi3, Marcelo A Wood1,2,6
ORCID iDs: Franklin G Garcia, Tracy L Fetterly, Jessica Childs, Angela Gomez-Arboledas
  1. Department of Neurobiology and Behavior, Charlie Dunlop School of Biological Sciences, University of California, Irvine, Irvine, CA 92697, USA
  2. Center for the Neurobiology of Learning and Memory, University of California, Irvine, Irvine, CA 92697, USA
  3. Department of Computer Science, Institute for Genomics and Bioinformatics, University of California, Irvine, Irvine, CA 92697, USA
  4. Institute for Memory Impairments and Neurological Disorders, University of California, Irvine, Irvine, CA, USA
  5. Department of Systems Pharmacology & Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
  6. Lead contact
Institutions: University of California, Irvine (United States); University of Pennsylvania (United States)
Journal: Cell reports, volume 45, issue 4, article 117158
Dates: published online 25 March 2026; in print March 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1016/j.celrep.2026.117158 · PMID 41886450 · PMCID PMC13189212 · OpenAlex W7140324049
Open access: gold, a free copy (OpenAlex)
Status: code on request (how the code is found and verified)
Categories: genetics / omics (modality)
Methods: Statistics, fMRI & imaging
Keywords: CBP, Long-term potentiation, LTP, LTM, Rna-seq, Long-term Memory, Olm, Creb-binding Protein, Dca1, Object Location Memory, Cp: Neuroscience, Cp: Genomics, Hippocampal Dorsal Ca1, Crest Y397, Crest/ss18l1, Cyber-t, Learning-induced Differentially Expressed Genes
Topic: Histone Deacetylase Inhibitors Research (Molecular Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: NCI NIH HHS (P30 CA062203); NIA NIH HHS (F32 AG077872, R01 AG076835, T32 AG000096); NIMH NIH HHS (R01 MH101491); National Institute on Aging; NIGMS NIH HHS (R01 GM123558); NIDA NIH HHS (R01 DA052465)
Citations: not cited yet (Europe PMC); 113 references in the paper
Research resources: IRDYE 680LT Rabbit secondary RRID:AB_10706309, anti-NeuN RRID:AB_10711040, Goat anti-Rabbit Alexa Fluor 568 RRID:AB_143157, Goat anti-Mouse Alexa Fluor 488 RRID:AB_143165, anti-HA RRID:AB_1549585, anti-CREST RRID:AB_2302517, DAPI RRID:AB_2307445, anti-CBP RRID:AB_3085692, IRDYE 800CW Mouse secondary RRID:AB_621842, anti-GAPDH RRID:AB_627679, Mouse: C57BL/6J RRID:IMSR_JAX:000664, Mouse: Cbpfl/fl RRID:IMSR_JAX:025178, DAVID Knowledgebase v2023q4 RRID:SCR_001881, RRID:SCR_002798, ImageJ RRID:SCR_003070, Leica Application Suite LAS X RRID:SCR_013673, Licor Image Studio Lite v5.2 RRID:SCR_013715, ANY-maze tracking software RRID:SCR_014289, RRID:SCR_014574, Biorender RRID:SCR_018361, AAV1-CMV-EV RRID:SCR_025573

Abstract

Chromatin-modifying and -remodeling machineries are important for learning-induced transcriptional activity, yet it remains unclear how they coordinate to drive de novo gene expression for memory formation. Here, we examine the transcription factor known as calcium-responsive transactivator (CREST) in memory formation, synaptic plasticity, and learning-induced gene expression. CREST is known to bind major chromatinmodifying and -remodeling machineries via interaction with CREB-binding protein (CBP) and brahma-related gene 1 (BRG1), respectively. In silico modeling of CREST identified tyrosine 397 (Y397) within the CBP-binding domain. Expression of a CREST Y397F point mutant impairs long-term potentiation and memory. Conversely, expression of a CREST Y397D point mutant enhances memory in a CBP-dependent manner. Differential gene expression analysis reveals distinct CREST Y397-regulated signatures during memory consolidation. CBP acts through CREB and post-translation modifications to affect memory, but the findings of this study argue for consideration of the CREST-CBP interaction and Y397 accessibility as factors in memory processes.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper says that its authors' code is available on request: it was not published with the paper, so there is nothing to verify.

The paper's code and data availability statement is in the Data section.

Tracing map

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Data

Datasets cited

Data and code availability

• The RNA-seq datasets generated in this study have been deposited to the NCBI Gene Expression Omnibus (GEO) under accession number GEO: GSE268687 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE268687) and are publicly available as of the date of publication.

• Original western blot images (Data S1) and the datapoints used to generate all graphs (Data S2) are provided as source data files.

• This paper does not report original code

• Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 30 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 17 authors, 17 keywords, 6 funders, 112 references, 21 RRIDs.

Cite

This paper

Garcia, F. G., de Albuquerque, M. F., Johnson, V., Kramár, E., Changizi, K., Abdelkarim, S., Augustynski, A. S., Matheos, D. P., Picone, J., Fetterly, T. L., Rodriguez, A., Childs, J., Gomez-Arboledas, A., Winter, J., Heller, E., Baldi, P., & Wood, M. A. (2026). The nBAF complex subunit CREST/SS18L1 regulates hippocampal memory processes via tyrosine 397 and histone acetyltransferase CBP. Cell reports, 45(4), 117158. https://doi.org/10.1016/j.celrep.2026.117158

BibTeX

@article{garcia2026nbaf,
author = {Garcia, Franklin G and de Albuquerque, Maria Farias and Johnson, Vanessa and Kramár, Enikö and Changizi, Kousha and Abdelkarim, Sherif and Augustynski, Agatha S and Matheos, Dina P and Picone, Joseph and Fetterly, Tracy L and Rodriguez, Alyssa and Childs, Jessica and Gomez-Arboledas, Angela and Winter, Julia and Heller, Elizabeth and Baldi, Pierre and Wood, Marcelo A},
title = {{The nBAF complex subunit CREST/SS18L1 regulates hippocampal memory processes via tyrosine 397 and histone acetyltransferase CBP}},
journal = {Cell reports},
year = {2026},
month = mar,
volume = {45},
number = {4},
pages = {117158},
publisher = {Cell Press},
issn = {2211-1247},
doi = {10.1016/j.celrep.2026.117158},
url = {https://doi.org/10.1016/j.celrep.2026.117158},
pmid = {41886450},
pmcid = {PMC13189212}
}

RIS

TY - JOUR
AU - Garcia, Franklin G
AU - de Albuquerque, Maria Farias
AU - Johnson, Vanessa
AU - Kramár, Enikö
AU - Changizi, Kousha
AU - Abdelkarim, Sherif
AU - Augustynski, Agatha S
AU - Matheos, Dina P
AU - Picone, Joseph
AU - Fetterly, Tracy L
AU - Rodriguez, Alyssa
AU - Childs, Jessica
AU - Gomez-Arboledas, Angela
AU - Winter, Julia
AU - Heller, Elizabeth
AU - Baldi, Pierre
AU - Wood, Marcelo A
TI - The nBAF complex subunit CREST/SS18L1 regulates hippocampal memory processes via tyrosine 397 and histone acetyltransferase CBP
T2 - Cell reports
J2 - Cell Rep
PY - 2026
DA - 2026/03/25
VL - 45
IS - 4
SP - 117158
SN - 2211-1247
PB - Cell Press
DO - 10.1016/j.celrep.2026.117158
UR - https://doi.org/10.1016/j.celrep.2026.117158
LA - en
ER -

CSL-JSON

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