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An improved method for high-purity isolation of oligodendrocytes from neonatal and adult mouse brain.

Overview

Authors: Peiyi Guo1, Elisabet Mandon2, Jie Wang3, Morgan Mooney1, Keith Reddig4, Paula Vo5, Madison C. Jerome5, Anoushka Lotun1, Jessica B. Spinelli6, Guangping Gao1, Dominic J. Gessler1,7
  1. Department of Genetic and Cellular Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA
  2. RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, USA
  3. College of Life Science, Nanjing Normal University, Nanjing, China
  4. Electron Microscopy Facility, Department of Radiology, University of Massachusetts Chan Medical School, Worcester, MA, USA
  5. Program in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA
  6. Howard Hughes Medical Institute, Program in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA
  7. Department of Neurosurgery, University of Massachusetts Chan Medical School, Worcester, MA, USA
Journal: iScience, volume 29, issue 5, article 115841
Dates: received 5 November 2025; accepted 24 March 2026; published online 22 April 2026
Type: Research article · Language: English
License: CC BY-NC-ND
Identifiers: DOI 10.1016/j.isci.2026.115841 · PMID 42111178 · PMCID PMC13157168 · OpenAlex W7155160093
Open access: gold, a free copy (OpenAlex)
Status: code on request
Categories: mouse (organism)
Methods: Statistics, Smoothing, state filtering, decompositions
Keywords: neuroscience, cell biology, biological sciences research methodologies
Topic: Neurogenesis and neuroplasticity mechanisms (Developmental Neuroscience, Neuroscience), according to OpenAlex
Funding: UMass Internal; NINDS (2R01NS076991-11A1, S10 OD025113-01, S10OD021580); National Center for Research Resources
Citations: not cited yet (Europe PMC); 54 references in the paper
Research resources: Mouse IgM, eFluor™ 660 RRID:AB_10598654, Rabbit monoclonal anti-Olig2 RRID:AB_10861310, Mouse monoclonal anti-APC (CC1) RRID:AB_2057371, RRID:AB_2340619, anti-Rat IgG (H + L), Rhodamine Red™-X RRID:AB_2340678, anti-Mouse IgG (H + L), Rhodamine Red™-X RRID:AB_2340835, anti-Mouse IgG (H + L), Alexa Fluor® 488 RRID:AB_2340850, Mouse monoclonal anti-O1, eFluor™ 660 RRID:AB_2574268, Rabbit monoclonal anti-NeuN RRID:AB_2651140, Human monoclonal anti-O4, PE RRID:AB_2733889, Human IgG1, PE RRID:AB_2733893, Human monoclonal anti-O4, APC RRID:AB_2751960, Rabbit monoclonal anti-AQP4 RRID:AB_2799571, RRID:AB_2801191, Mouse monoclonal anti-O4 microBeads RRID:AB_2847907, Mouse monoclonal anti-O4 RRID:AB_357617, Mouse IgG1, Alexa Fluor 488 RRID:AB_493438, Rat monoclonal anti-PDGFRα RRID:AB_529483, Rabbit polyclonal anti-Iba1 RRID:AB_839504, Rat monoclonal anti-MBP RRID:AB_94975, C57BL/6J RRID:IMSR_JAX:000664

Abstract

The abstract is not reproduced here: the paper's license (CC BY-NC-ND) does not allow it. Read it in the paper, at the publisher or on Europe PMC.

Code

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Data

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Code and data availability statement

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Read it in the paper: doi.org/10.1016/j.isci.2026.115841.

Versions

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Version 1, 29 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 11 authors, 3 keywords, 3 funders, 54 references, 21 RRIDs.

Cite

This paper

Guo, P., Mandon, E., Wang, J., Mooney, M., Reddig, K., Vo, P., Jerome, M. C., Lotun, A., Spinelli, J. B., Gao, G., & Gessler, D. J. (2026). An improved method for high-purity isolation of oligodendrocytes from neonatal and adult mouse brain. iScience, 29(5), 115841. https://doi.org/10.1016/j.isci.2026.115841

BibTeX

@article{guo2026improved,
author = {Guo, Peiyi and Mandon, Elisabet and Wang, Jie and Mooney, Morgan and Reddig, Keith and Vo, Paula and Jerome, Madison C. and Lotun, Anoushka and Spinelli, Jessica B. and Gao, Guangping and Gessler, Dominic J.},
title = {{An improved method for high-purity isolation of oligodendrocytes from neonatal and adult mouse brain}},
journal = {iScience},
year = {2026},
month = apr,
volume = {29},
number = {5},
pages = {115841},
publisher = {Elsevier},
issn = {2589-0042},
doi = {10.1016/j.isci.2026.115841},
url = {https://doi.org/10.1016/j.isci.2026.115841},
pmid = {42111178},
pmcid = {PMC13157168}
}

RIS

TY - JOUR
AU - Guo, Peiyi
AU - Mandon, Elisabet
AU - Wang, Jie
AU - Mooney, Morgan
AU - Reddig, Keith
AU - Vo, Paula
AU - Jerome, Madison C.
AU - Lotun, Anoushka
AU - Spinelli, Jessica B.
AU - Gao, Guangping
AU - Gessler, Dominic J.
TI - An improved method for high-purity isolation of oligodendrocytes from neonatal and adult mouse brain
T2 - iScience
J2 - iScience
PY - 2026
DA - 2026/04/22
VL - 29
IS - 5
SP - 115841
SN - 2589-0042
PB - Elsevier
DO - 10.1016/j.isci.2026.115841
UR - https://doi.org/10.1016/j.isci.2026.115841
LA - en
ER -

CSL-JSON

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