OSCR

JACUZI-SD: An automated, high-throughput, minimally stressful approach to sleep depriving larval zebrafish.

Overview

Authors: Leah J Elias1, Harrison Khoo2, François Kroll3,4, Caroline Zhang1, Soojung C Hur2,5, Jason Rihel4, Seth Blackshaw1,6,7,8,9
  1. Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA
  2. Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA
  3. Sorbonne Université, INSERM U968, CNRS UMR 7210, Institut de la Vision, Paris, France
  4. Department of Cell and Developmental Biology, University College London, London, UK
  5. Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
  6. Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
  7. Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
  8. Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA
  9. Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Institutions: Johns Hopkins University (United States); Johns Hopkins Medicine (United States); Centre National de la Recherche Scientifique (France); Institut de la Vision (France); Inserm (France); Sorbonne Université (France); University College London (United Kingdom)
Journal: iScience, volume 29, issue 6, article 115954
Dates: received 4 April 2025; accepted 27 April 2026; published online 30 April 2026
Type: Research article · Language: English
License: CC BY-NC-ND
Identifiers: DOI 10.1016/j.isci.2026.115954 · PMID 42181281 · PMCID PMC13196093 · OpenAlex W4409149128
Open access: gold, a free copy (OpenAlex)
Status: empty repository
Categories: zebrafish (organism)
Methods: Statistics
Keywords: biological sciences
Topic: Zebrafish Biomedical Research Applications (Cell Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: Johns Hopkins University; Fondation pour la Recherche Médicale (SPF202309017505); Wellcome Trust (217150/Z/19/Z); Jane Coffin Childs Memorial Fund for Medical Research; National Institute of Mental Health (R01MH126676)
Citations: cited by 1 paper (Europe PMC); 41 references in the paper

Abstract

The abstract is not reproduced here: the paper's license (CC BY-NC-ND) does not allow it. Read it in the paper, at the publisher or on Europe PMC.

Code

No file of the authors' code could be read here: it is described below, and read at its source.

supp:PMC13196093/mmc2.zip

License: none: the authors keep all their rights
State: the link answers, verified on 30 September 2026
Evidence: files inventoried
Size: 0 files, 0 scripts
Software Heritage: not checked
Found in: the supplementary material
Not found: README, license file, CITATION.cff, environment file, tests, continuous integration, documentation
Availability: 1 check, the latest on 30 September 2026: the link answers (HTTP 200)
  • 30 September 2026: the link answers (HTTP 200)

The paper's code and data availability statement is in the Data section.

Tracing map

Proposed by the machine: these links were found in the paper and verified at the source, without human review. The map will receive a Zenodo DOI once one of the paper's authors has validated it with their ORCID.

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  • 1 repository of the authors' code, each at its verified commit, with its license and how the link was found in the paper;
  • 0 scripts, each with its path and the digest of its content;
  • no match between paragraphs and code yet;
  • neither the text of the paper nor the code itself.

Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.

Data

No dataset and no data link were found in the paper.

Availability statements

The paper has a data availability statement and a code and data availability statement. Its license (CC BY-NC-ND) does not allow reproducing them here; in short, from what the harvester recognized in them:

  • they say that the data are available on request
  • they say that the code is available on request

Read them in the paper: doi.org/10.1016/j.isci.2026.115954.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 30 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 7 authors, 1 keyword, 5 funders, 41 references.

Cite

This paper

Elias, L. J., Khoo, H., Kroll, F., Zhang, C., Hur, S. C., Rihel, J., & Blackshaw, S. (2026). JACUZI-SD: An automated, high-throughput, minimally stressful approach to sleep depriving larval zebrafish. iScience, 29(6), 115954. https://doi.org/10.1016/j.isci.2026.115954

BibTeX

@article{elias2026jacuzi,
author = {Elias, Leah J and Khoo, Harrison and Kroll, François and Zhang, Caroline and Hur, Soojung C and Rihel, Jason and Blackshaw, Seth},
title = {{JACUZI-SD: An automated, high-throughput, minimally stressful approach to sleep depriving larval zebrafish}},
journal = {iScience},
year = {2026},
month = apr,
volume = {29},
number = {6},
pages = {115954},
publisher = {Elsevier},
issn = {2589-0042},
doi = {10.1016/j.isci.2026.115954},
url = {https://doi.org/10.1016/j.isci.2026.115954},
pmid = {42181281},
pmcid = {PMC13196093}
}

RIS

TY - JOUR
AU - Elias, Leah J
AU - Khoo, Harrison
AU - Kroll, François
AU - Zhang, Caroline
AU - Hur, Soojung C
AU - Rihel, Jason
AU - Blackshaw, Seth
TI - JACUZI-SD: An automated, high-throughput, minimally stressful approach to sleep depriving larval zebrafish
T2 - iScience
J2 - iScience
PY - 2026
DA - 2026/04/30
VL - 29
IS - 6
SP - 115954
SN - 2589-0042
PB - Elsevier
DO - 10.1016/j.isci.2026.115954
UR - https://doi.org/10.1016/j.isci.2026.115954
LA - en
ER -

CSL-JSON

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The tracing map gets a citation of its own once an author has validated it and it has a DOI.

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