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Long-term effects of adolescent risperidone treatment on the mouse cortex.

Code ↔ Paper

4 matches between paragraphs of the paper and lines of its authors' code, computed by the harvester (lexical-v1). Click a colored paragraph or line to see its counterpart.

The 4 matches
  1. [1] § Materials and methods › SnRNA-seq statistics ↔ Antipsychotic_github.Rmd, lines 931–1055 · score 0.85 · FDR threshold, Cellular Component, EnrichGO, Biological Process, Molecular Function, CC
  2. [2] § Results › SnRNA-seq analysis shows transcriptional changes associated with synaptic function in risperidone-treated mouse cortex ↔ Antipsychotic_github.Rmd, lines 2263–2404 · score 0.72 · log2FC, Upregulated genes, downregulated genes, GO enrichment, log10 adjusted, MF
  3. [3] § Results › High-dimensional WGCNA identifies key gene modules associated with risperidone treatment ↔ Antipsychotic_github.Rmd, lines 3495–3592 · score 0.64 · co expression network, module assignment, hub gene, glutamatergic neurons, Dendogram, WGCNA
  4. [4] § Results › High-dimensional WGCNA identifies key gene modules associated with risperidone treatment ↔ Antipsychotic_github.Rmd, lines 3384–3493 · score 0.61 · M10, GO terms, hdWGCNA, M8, M9, M6

Paper

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The authors' code

R Markdown · 5,888 lines · 196 KB · no license · 4 matches

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It can be read at the source: Antipsychotic_github.Rmd.

Overview

Authors: Abneil D Alicea-Pauneto1,2, Hyowon Choi1,2,3, Wenyu Zhang1,2, Xinjian Li4, Laurence A Busque1,2, Mingxuan Li5, Andrew Wu1,2, Evan Zhang6, Adam D Marc1,2, Jonathan Preall6, Kuan Hong Wang7, Robert E Featherstone8, Steven J Siegel8, Chang-Gyu Hahn1,2,3, Karin E Borgmann-Winter1,2,3
ORCID iDs: Kuan Hong Wang
  1. Department of Neuroscience, Thomas Jefferson University, Philadelphia, PA USA
  2. Vickie & Jack Farber Institute for Neuroscience, Thomas Jefferson University, Philadelphia, PA USA
  3. Department of Psychiatry and Human Behavior, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA USA
  4. Department of Neurology of the Second Affiliated Hospital, Interdisciplinary Institute of Neuroscience and Technology, Zhejiang University School of Medicine, Hangzhou, China
  5. Department of Computer Science, Hertford College, University of Oxford, Oxford, UK
  6. Cold Spring Harbor Laboratory Cancer Center, Cold Spring Harbor, NY USA
  7. Department of Neuroscience, University of Rochester Medical Center, Rochester, NY USA
  8. Department of Psychiatry and the Behavioral Sciences, University of Southern California, Los Angeles, CA USA
Institutions: Thomas Jefferson University (United States); Second Affiliated Hospital of Zhejiang University (China); Zhejiang University (China); University of Oxford (United Kingdom); Cancer Center at Cold Spring Harbor Laboratory; University of Rochester Medicine (United States); University of Rochester (United States); University of Southern California (United States)
Journal: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, volume 51, issue 10, pages 1844-1854
Dates: received 23 January 2026; accepted 23 June 2026; published online 8 July 2026; in print September 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1038/s41386-026-02483-2 · PMID 42420423 · PMCID PMC13486667 · OpenAlex W7167711737
Open access: hybrid, a free copy (OpenAlex)
Status: code verified
Categories: genetics / omics (modality), human (organism), mouse (organism), schizophrenia / psychosis (population)
Methods: Statistics, Smoothing, state filtering, decompositions, fMRI & imaging, Single-unit activity, calcium imaging, Spectral & time-frequency
Keywords: Synaptic development, Cellular neuroscience, Genetics of the nervous system
MeSH: Antipsychotic Agents*, Cerebral Cortex*, Risperidone*, Animals, Anxiety, Female, Male, Mice, Mice, Inbred C57BL, Neurons (* major topic)
Topic: Neuroscience and Neuropharmacology Research (Cellular and Molecular Neuroscience, Neuroscience), according to OpenAlex
Funding: U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH) (RO1MH138995, RO1MH116463); NIGMS NIH HHS (T32 GM008562); NIMH NIH HHS (R01 MH138995, R01 MH116463); U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) (T32GM008562)
Citations: not cited yet (Europe PMC); 56 references in the paper

Abstract

Since the introduction of second-generation antipsychotics, antipsychotics have been increasingly prescribed for children and adolescents, raising concerns about their long-term impact on neurodevelopment. Antipsychotics block dopaminergic and serotonergic receptors, potentially disrupting the maturation of neurocognitive processes, which is a public health concern. Previous studies have reported that adolescent antipsychotic treatment can cause persistent neurocognitive dysfunction in rodents, yet the neurobiological underpinnings remain unknown. To address this, we administered risperidone, a commonly used antipsychotic, to C57BL/6 mice during adolescence (3 to 6 weeks of age) and examined behavioral and neurobiological outcomes nine weeks post-treatment. Risperidone-treated mice exhibited subtle deficits in behavioral correlates of anxiety-like behavior. In vivo, two-photon calcium imaging of cortical neurons revealed a remarkable increase in the amplitude of calcium events with subtle sex-specific changes in the frequency, consistent with increased neuronal excitability. Single-nucleus RNA-sequencing (snRNA-seq) analyses showed widespread reductions in transcripts for voltage-sensitive and inwardly rectifying potassium channels in both pyramidal neurons and interneurons. Additionally, both cell types exhibited reduced Grin2a and Grin2b, as well as scaffolding proteins, indicative of weakened synaptic connectivity between excitatory and inhibitory neurons. Interestingly, we observed sex-dependent differences in the directionality of correlation between certain gene co-expression modules and risperidone treatment. Our results suggest that adolescent risperidone treatment induces lasting transcriptomic and functional changes associated with altered excitatory-inhibitory neuronal interactions that may underline cognitive and behavioral dysregulations.

Reproduced under the paper's license (CC BY), from the paper cited above.

Repository

Its files are read in the Code ↔ Paper reader above, with 4 matches between paragraphs and lines of code.

Hahn-BorgmannWinter/Adolescent_antipsychotics

License: none: the authors keep all their rights
State: the link answers, verified on 27 September 2026
Evidence: files inventoried
Commit: b768f3a632e45fccec74c82fd5084bdb451ccd50, 26 January 2026
Languages: R (1)
Size: 2 files, 1 script
Software Heritage: not archived
Found in: “Data availability”
Holds: README, 1 notebook
Not found: license file, CITATION.cff, environment file, tests, continuous integration, documentation
Tools: clusterProfiler (1 file), cowplot (1 file), DESeq2 (1 file), ggplot2 (1 file), igraph (1 file), patchwork (1 file), pheatmap (1 file), reshape2 (1 file), Seurat (1 file), tidyverse (1 file), WGCNA (1 file)
Availability: 1 check, the latest on 27 September 2026: the link answers
  • 27 September 2026: the link answers
2 files, not copied: shown from their source

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At the source: github.com/Hahn-BorgmannWinter/Adolescent_antipsychotics

The paper's code and data availability statement is in the Data section.

Tracing map

Proposed by the machine: these links were found in the paper and verified at the source, without human review. The map will receive a Zenodo DOI once one of the paper's authors has validated it with their ORCID.

What the map holds:

  • 1 repository of the authors' code, each at its verified commit, with its license and how the link was found in the paper;
  • 1 script, each with its path and the digest of its content;
  • 4 matches between paragraphs of the paper and lines of the code (method lexical-v1);
  • neither the text of the paper nor the code itself.

Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.

Data

Datasets cited

Data availability

The sequence and raw count matrices generated and analyzed for snRNA-seq experiments are deposited to the Gene Expression Omnibus, GSE316091 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316091) (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316091). All code used to generate results for snRNA-seq analysis is available at: https://github.com/Hahn-BorgmannWinter/Adolescent_antipsychotics.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

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Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 15 authors, 3 keywords, 10 MeSH terms, 4 funders, 56 references.

Cite

This paper

Alicea-Pauneto, A. D., Choi, H., Zhang, W., Li, X., Busque, L. A., Li, M., Wu, A., Zhang, E., Marc, A. D., Preall, J., Wang, K. H., Featherstone, R. E., Siegel, S. J., Hahn, C.-G., & Borgmann-Winter, K. E. (2026). Long-term effects of adolescent risperidone treatment on the mouse cortex. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 51(10), 1844-1854. https://doi.org/10.1038/s41386-026-02483-2

BibTeX

@article{aliceapauneto2026long,
author = {Alicea-Pauneto, Abneil D and Choi, Hyowon and Zhang, Wenyu and Li, Xinjian and Busque, Laurence A and Li, Mingxuan and Wu, Andrew and Zhang, Evan and Marc, Adam D and Preall, Jonathan and Wang, Kuan Hong and Featherstone, Robert E and Siegel, Steven J and Hahn, Chang-Gyu and Borgmann-Winter, Karin E},
title = {{Long-term effects of adolescent risperidone treatment on the mouse cortex}},
journal = {Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology},
year = {2026},
month = jul,
volume = {51},
number = {10},
pages = {1844--1854},
publisher = {Nature Publishing Group},
issn = {0893-133X},
doi = {10.1038/s41386-026-02483-2},
url = {https://doi.org/10.1038/s41386-026-02483-2},
pmid = {42420423},
pmcid = {PMC13486667}
}

RIS

TY - JOUR
AU - Alicea-Pauneto, Abneil D
AU - Choi, Hyowon
AU - Zhang, Wenyu
AU - Li, Xinjian
AU - Busque, Laurence A
AU - Li, Mingxuan
AU - Wu, Andrew
AU - Zhang, Evan
AU - Marc, Adam D
AU - Preall, Jonathan
AU - Wang, Kuan Hong
AU - Featherstone, Robert E
AU - Siegel, Steven J
AU - Hahn, Chang-Gyu
AU - Borgmann-Winter, Karin E
TI - Long-term effects of adolescent risperidone treatment on the mouse cortex
T2 - Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
J2 - Neuropsychopharmacology
PY - 2026
DA - 2026/07/08
VL - 51
IS - 10
SP - 1844
EP - 1854
SN - 0893-133X
PB - Nature Publishing Group
DO - 10.1038/s41386-026-02483-2
UR - https://doi.org/10.1038/s41386-026-02483-2
LA - en
ER -

CSL-JSON

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