Ribo-ITP enables identification of translons from limited input samples.
The 7 matches
- [1] § Methods › Correlation of ribosome occupancy of translons vs. CDS ↔ figures/fig4.R, lines 245–330 · score 0.75 · Wilcoxon signed rank, partial Spearman, RNA seq, coefficients, correlation, CPM
- [2] § Methods › Identification of translated ORFs ↔ pipeline/ORFPipeline.py, lines 403–434 · score 0.67 · nucleotide periodicity, chi squared, threshold, start codons, candidate, APPRIS
- [3] § Methods › Correlation of ribosome occupancy of translons vs. CDS ↔ figures/fig4.R, lines 245–330 · score 0.59 · Gini coefficient, partial Spearman, correlation, CPM, cell, CDS
- [4] § Results › Expression patterns of non-canonical translons ↔ figures/fig4.R, lines 1–67 · score 0.58 · neural stem cells, heart, neurons, brain, tissues, embryonic
- [5] § Methods › Cell culture ↔ figures/fig4.R, lines 1–67 · score 0.54 · mouse embryonic stem, fetal, cells
- [6] § Methods › Amino acid composition comparison between translons and CDS regions ↔ figures/fig3.R, lines 133–180 · score 0.52 · CDS frequencies, amino acid, composition, genes
- [7] § Results › Ribo-ITP enables identification of non-canonical translation events from microdissected mouse hippocampus ↔ pipeline/ribobaseFTFilter.py, lines 204–214 · score 0.51 · Fourier transform, RiboBase, database, filtered, pipeline, transcriptome
Paper
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The authors' code
R · 342 lines · 15 KB · no license · 4 matches
- library(tidyverse)
- library(ppcor)
- library(ggplot2)
- library(scales)
- library(readr)
- library(dplyr)
- library(cowplot)
- library(viridis)
- library(DescTools)
- library("ggExtra")
- # Load files
- orfE = read_csv("outputFTembryoORF_comp.csv", col_names = TRUE)
- orfN = read_csv("outputFTneuralORF_comp.csv", col_names = TRUE)
- dataE = read_csv("outputRNASeqORFembryo.csv", col_names = TRUE)
- dataN = read_csv("outputRNASeqORFneural.csv", col_names = TRUE)
- # Add Type
- embryo_data <- left_join(dataE, dplyr::select(orfE, Gene, Start, Stop, Type),
- by = c("Gene", "Start", "Stop"))
- neural_data <- left_join(dataN, dplyr::select(orfN, Gene, Start, Stop, Type),
- by = c("Gene", "Start", "Stop"))
- # Label and combine datasets
- neural_data <- neural_data %>% mutate(Context = "Neural")
- embryo_data <- embryo_data %>% mutate(Context = "Embryo")
- combined_data <- bind_rows(neural_data, embryo_data)
- reads_CPM <- data.frame(reads_CPM = ((combined_data$`ORF Reads` / combined_data$`Experiment Reads`) * 1000000))
- combined_data <- cbind(combined_data, reads_CPM)
- gene_CPM <- data.frame(gene_CPM = ((combined_data$`CDS Reads` / combined_data$`Experiment Reads`) * 1000000))
- combined_data <- cbind(combined_data, gene_CPM)
- combined_data <- combined_data %>% filter(!is.na(reads_CPM) & !is.na(gene_CPM))
- # Combine and simplify cell lines
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("Liver cells", "liver tissue", "liver")] <- "Liver"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("ES cell line A3-1", "RF8 mouse embryonic stem cell line", "Embryonic stem cells", "embryonic stem cells", "A3-1", "v6.5", "R1 mouse embryonic stem cells", "ESC line CGR8", "E14", "E14Tg2a")] <- "ESC"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("brain neural stem cells", "neural tube")] <- "NSC"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("inguinal white fat", "subcutaneous white fat", "epididymal white fat", "interscapular brown fat")] <- "Fat"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("Bone marrow derived primary dendritic cells", "Bone marrow derived dendritic cells", "Bone marrow derived regulatory dendritic cells", "Flt3L-DC")] <- "Bone marrow dendritic"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("3T3", "CD4 T-cells", "resting state T cells")] <- "T cells"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("Forebrain tissue (WT E14.5 mouse embryos)", "Forebrain tissue (Elp3cKO E14.5 mouse embryos)", "hippocampal", "cortical tissue", "brain cortex", "hippocampal cortex", "Cerebellum", "Fetal cortex", "dentate gyrus", "hemisphere")] <- "brain"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("Bone marrow derived macrophage", "RAW264", "peritoneal macrophage cells", "RAW264.7")] <- "Macrophage"
- combined_data[combined_data == "RAW264.7"] <- "RAW264"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("Mouse back skins", "primary epidermis", "Epidermal basal cells")] <- "Skin"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("Quadriceps muscle", "tibialis anterior tissue", "gastrocnemius tissue", "forelimbs")] <- "Skeletal muscle"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("ES cell derived neurons", "Primary cortical neurons", "Neurons (DIV 8) derived from CGR8 ES cells", "Cortical neuron", "Striatal cells")] <- "Neuron"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("EL4", "T-ALL")] <- "Leukemia"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("Splenic B cells", "follicular B cells", "primary splenic B cells", "resting state B cells")] <- "B cells"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("1 cell", "2 cell", "4 cell", "morula", "blastocyst")] <- "Preimplantation embryo"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("duodenum", "Ileum")] <- "Small intestine"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("KH2", "P19")] <- "Tetracarcinoma"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("Spontaneously Immortalized Mouse Keratinocyte Cult", "Primary Keratinocytes")] <- "Keratinocyte"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("Cardiac (left ventricle)", "heart")] <- "Heart"
- combined_data$Cell_Line[combined_data$Cell_Line %in% c("testis", "testes")] <- "Testes"
- combined_data$Cell_Line[combined_data$Cell_Line == "mouse eye"] <- "Eye"
- combined_data$Cell_Line[combined_data$Cell_Line == "fat"] <- "Fat"
- combined_data$Cell_Line[combined_data$Cell_Line == "brain"] <- "Brain"
- combined_data$Cell_Line[combined_data$Cell_Line == "embryo"] <- "Embryo"
- combined_data$Cell_Line[combined_data$Cell_Line == "lung"] <- "Lung"
- combined_data$Cell_Line[combined_data$Cell_Line == "kidney"] <- "Kidney"
- combined_data$Cell_Line[combined_data$Cell_Line == "liver"] <- "Liver"
- combined_data$Cell_Line[combined_data$Cell_Line == "pancreas"] <- "Pancreas"
- combined_data$Cell_Line[combined_data$Cell_Line == "skeletal muscle"] <- "Skeletal muscle"
- #simplify names
- combined_data[combined_data == "Dorsal section of lumbar spinal cord"] <- "Spinal cord"
- combined_data[combined_data == "KRPC-A cells (from gen. eng. murine tumors)"] <- "KRPC-A"
- combined_data[combined_data == "skin squamous tumours (skin papilloma)"] <- "Skin papilloma"
- combined_data[combined_data == "embryonic fibroblast"] <- "MEF"
- combined_data[combined_data == "lymphoid ba/f3 cells"] <- "Lymphoid"
- # Capitalize
- combined_data[combined_data == "neutrophils"] <- "Neutrophils"
- combined_data[combined_data == "spleen"] <- "Spleen"
- # Define ORF type
- combined_data <- combined_data %>%
- mutate(ORF_Class = ifelse(str_detect(Type, "dORF"), "dORF", "uORF")) %>%
- mutate(Group = paste(Context, ORF_Class))
- ### Themes ###
- theme_custom <- theme_minimal() +
- theme(
- panel.grid = element_blank(),
- panel.border = element_blank(),
- axis.line.x = element_line(color = "black"),
- axis.line.y = element_line(color = "black"),
- axis.ticks = element_blank(),
- axis.text = element_text(color = "black", size = 7),
- axis.title = element_text(size = 8),
- legend.title=element_text(size=7),
- legend.text=element_text(size=7),
- legend.key.size = unit(0.5, "lines")
- )
- theme_custom_x0 <- theme_minimal() +
- theme(
- panel.grid = element_blank(),
- panel.border = element_blank(),
- axis.line.x = element_blank(),
- axis.line.y = element_line(color = "black"),
- axis.ticks = element_blank(),
- axis.text = element_text(color = "black", size = 7),
- axis.title = element_text(size = 7),
- legend.title=element_text(size=8),
- legend.text=element_text(size=7),
- legend.key.size = unit(0.2, "lines"),
- legend.key.height = unit(0.2, "lines"),
- legend.key.width = unit(0.2, "lines"),
- legend.spacing.y = unit(0.1, "lines")
- )
- ### Dot Plot of Translation Level ###
- # Collapse to one value per Gene/Start/Stop/Cell_Line
- per_orf <- combined_data %>%
- group_by(Cell_Line, Group, Gene, Start, Stop) %>%
- summarise(reads = mean(reads_CPM, na.rm = TRUE), .groups = "drop")
- plot_data <- per_orf %>%
- group_by(Cell_Line, Group) %>%
- summarise(
- Med_Reads = median(reads, na.rm = TRUE),
- Pct_Translated = mean(reads > 0, na.rm = TRUE) * 100, # percent non-zero
- .groups = "drop"
- )
- # Create a data frame for shading
- background_rects <- data.frame(
- Group = factor(c("Neural dORF", "Neural uORF", "Embryo dORF", "Embryo uORF"),
- levels = levels(factor(plot_data$Group))),
- fill_color = c("steelblue", "turquoise", "brown", "tomato")
- )
- plot_data <- plot_data %>%
- mutate(
- Dataset = case_when(
- str_detect(Group, "Neural") ~ "Neural",
- str_detect(Group, "Embryo") ~ "Embryo"
- ),
- ORF_Class = case_when(
- str_detect(Group, "uORF") ~ "uORF",
- str_detect(Group, "dORF") ~ "dORF"
- )
- )
- neural_order <- plot_data %>%
- filter(Dataset == "Neural") %>%
- group_by(Cell_Line) %>%
- summarise(med = median(Med_Reads),
- pct_med = median(Pct_Translated)) %>%
- arrange(desc(med), desc(pct_med)) %>%
- pull(Cell_Line)
- plot_data$Cell_Line <- factor(plot_data$Cell_Line, levels = neural_order)
- # Plot
- p_dotplot <- ggplot(plot_data, aes(x = Cell_Line, y = Group)) +
- geom_rect(data = background_rects,
- aes(ymin = as.numeric(Group) - 0.5,
- ymax = as.numeric(Group) + 0.5),
- xmin = -Inf, xmax = Inf,
- fill = background_rects$fill_color,
- alpha = 0.05,
- inherit.aes = FALSE) +
- geom_point(aes(size = Pct_Translated, fill = Med_Reads+0.001), shape = 21,
- stroke = 0.2,
- color = "black") +
- scale_fill_viridis(
- option = "plasma",
- name = "Median reads\n(CPM)",
- trans = "log10",
- labels = scales::label_number(accuracy = 0.001)
- ) +
- scale_size(range = c(1, 3), name = "Percent\ntranslated") +
- labs(x = NULL, y = NULL) +
- theme_custom +
- theme(
- axis.text.x = element_text(angle = 90, hjust = 1),
- legend.box.margin = margin(t = 50)
- )
- ### Embryo violin plot ###
- plot_partial_correlation <- function(data, fillColor, orftype, xPos, yOff) {
- data <- data %>% filter(!is.na(RNASeq_Exp))
- data$Name = paste0(data$Gene,"_",data$Start)
- data$RNA_CPM = data$RNASeq_Gene/data$RNASeq_Exp * 10^6
- # Run Spearman partial correlation for each ORF
- unique_orfs = unique(data$Name)
- outcorr <- data.frame(Name = unique_orfs, R = NA, p = NA)
- for (orf in unique_orfs) {
- orf_data <- data %>% filter(Name == orf)
- if (sum(orf_data$gene_CPM) == 0) {
- outcorr[outcorr$Name == orf, "R"] <- NA
- outcorr[outcorr$Name == orf, "p"] <- NA
- next
- }
- test <- pcor.test(orf_data$reads_CPM, orf_data$gene_CPM, orf_data$RNA_CPM, method = "spearman")
- outcorr[outcorr$Name == orf, "R"] <- test$estimate
- outcorr[outcorr$Name == orf, "p"] <- test$p.value
- }
- outcorr$padj <- p.adjust(outcorr$p, method = "fdr")
- mean_R <- mean(outcorr$R, na.rm = TRUE)
- # Run Wilcoxon signed-rank test
- rhos <- outcorr$R[!is.na(outcorr$R)]
- wilcox_p <- wilcox.test(rhos, mu = 0, alternative = "two.sided")$p.value
- print(wilcox_p)
- # Generate violin plot
- plot_data = outcorr %>% filter(!is.na(R))
- p = ggplot(plot_data, aes(x = "", y = R)) +
- geom_violin(trim = TRUE, fill = fillColor) +
- geom_hline(yintercept = mean_R, linetype = "dashed", color = "black") +
- annotate("text", x = xPos, y = mean_R + yOff, label = bquote(bar(rho) == .(round(mean_R, 2))), color = "black", size = 2)+
- labs(x = "", y = expression("Spearman " * rho)) +
- geom_hline(yintercept = 0, color = "black", linewidth = 0.5) +
- coord_cartesian(xlim = c(1, 1.3)) +
- theme_custom_x0
- return(p)
- }
- dataE = combined_data[combined_data$Context == "Embryo", ]
- uorfE <- dataE %>% filter(!str_detect(Type, "dORF"))
- dorfE <- dataE %>% filter(str_detect(Type, "dORF"))
- p_uCorr = plot_partial_correlation(uorfE, "turquoise", "uORF", 1.69, 0.037)
- p_dCorr = plot_partial_correlation(dorfE, "steelblue", "dORF", 1.61, 0.025)
- ### Neural specificity analysis + plot ###
- specificity_correlation <- function(data, orftype, fillColor) {
- # cell line average
- avg <- data %>%
- group_by(Cell_Line, Gene, Start, Stop) %>%
- summarise(`ORF Reads` = mean(reads_CPM), .groups = "drop") %>%
- mutate(Name = paste0(Gene, "_", Start))
- avg_wide <- avg %>%
- dplyr::select(Name, Cell_Line, `ORF Reads`) %>%
- pivot_wider(names_from = Cell_Line, values_from = `ORF Reads`, values_fill = 0)
- # Compute Gini coefficient for each ORF across cell lines
- expr_cols <- setdiff(names(avg_wide), "Name")
- avg_wide$Gini <- apply(avg_wide[, expr_cols], 1, function(x) {
- if (sum(x) == 0) return(NA)
- Gini(x, na.rm = TRUE)
- })
- data <- data %>% filter(!is.na(RNASeq_Exp))
- data$Name = paste0(data$Gene,"_",data$Start)
- data$RNA_CPM = data$RNASeq_Gene/data$RNASeq_Exp * 10^6
- # Run Spearman partial correlation for each ORF
- unique_orfs = unique(data$Name)
- outcorr <- data.frame(Name = unique_orfs, R = NA, p = NA)
- for (orf in unique_orfs) {
- orf_data <- data %>% filter(Name == orf)
- if (sum(orf_data$gene_CPM) == 0) {
- outcorr[outcorr$Name == orf, "R"] <- NA
- outcorr[outcorr$Name == orf, "p"] <- NA
- next
- }
- test <- pcor.test(orf_data$reads_CPM, orf_data$gene_CPM, orf_data$RNA_CPM, method = "spearman")
- outcorr[outcorr$Name == orf, "R"] <- test$estimate
- outcorr[outcorr$Name == orf, "p"] <- test$p.value
- }
- outcorr$padj <- p.adjust(outcorr$p, method = "fdr")
- # Run Wilcoxon signed-rank test
- rhos <- outcorr$R[!is.na(outcorr$R)]
- wilcox_p <- wilcox.test(rhos, mu = 0, alternative = "two.sided")$p.value
- # Spearman for Gini vs. ORF-CDS rho
- merged <- left_join(avg_wide[, c("Name", "Gini")], outcorr, by = "Name") %>%
- filter(!is.na(Gini) & !is.na(R))
- included_names <- merged$Name
- all_names <- intersect(avg_wide$Name, outcorr$Name)
- excluded_names <- setdiff(all_names, included_names)
- # Print excluded ORFs - usually due to no CDS reads in all datasets
- if (length(excluded_names) > 0) {
- message(length(excluded_names), " ORFs were excluded from the final correlation due to missing Gini or R.")
- print(excluded_names)
- }
- cor_result <- cor.test(merged$Gini, merged$R, method = "spearman")
- print(cor_result)
- # Plot Gini vs Spearman rho
- merged$Group <- orftype
- p <- ggplot(merged, aes(x = Gini, y = R, color = Group)) +
- geom_point(alpha = 0.9, size = 1) +
- scale_color_manual(values = setNames(fillColor, orftype)) +
- labs(
- x = "Gini Coefficient",
- y = expression("Partial Spearman " * rho)
- ) +
- annotate(
- "text",
- x = 0.05, y = max(merged$R),
- label = bquote(rho == .(round(cor_result$estimate, 2))),
- hjust = 0, size = 2.5
- ) +
- theme_custom_x0 +
- theme(
- legend.position = "none"
- ) +
- geom_hline(yintercept = 0, color = "black", linewidth = 0.5)
- p = ggMarginal(p, type = "density", groupFill = TRUE, margins = "y")
- return(list(plot = p, corr_df = merged, corr_res = cor_result))
- }
- dataN = combined_data[combined_data$Context == "Neural", ]
- uorfN <- dataN %>% filter(!str_detect(Type, "dORF"))
- dorfN <- dataN %>% filter(str_detect(Type, "dORF"))
- outputU = specificity_correlation(uorfN, "uORF", "tomato")
- outputD = specificity_correlation(dorfN, "dORF", "brown")
- ### Arrangement ###
- row2 = plot_grid(p_uCorr, p_dCorr, outputU$plot, outputD$plot, "", labels = c("B", "C", "D", "E", ""), label_size = 10, ncol = 5, rel_widths = c(1,1,1,1,0.05))
- fig4 = plot_grid(p_dotplot, row2, ncol = 1, labels = c("A", ""), label_size = 10, rel_heights = c(1.5, 1))
- ggsave("fig4.pdf", fig4, width = 6.3, height = 4.3, units = "in")
fig4.R at commit c46cd1f, no license · at the source
Overview
- Department of Molecular Biosciences, University of Texas at Austin,Austin, TX USA
- Departments of Neurology and Neuroscience, Center for Learning and Memory, Dell Medical School, University of Texas at Austin,Austin, TX USA
Abstract
The abstract is not reproduced here: the paper's license (CC BY-NC-ND) does not allow it. Read it in the paper, at the publisher or on Europe PMC.
Repository
Its files are read in the Code ↔ Paper reader above, with 7 matches between paragraphs and lines of code.
up114/translons
c46cd1fb214b3e79542e087e764b4ab0af6c2406, 13 February 2026Availability: 1 check, the latest on 27 September 2026: the link answers
- 27 September 2026: the link answers
9 files
- analysis/
addinfo.py , Python, 126 lines - analysis/
ribobaseRNASeq.py , Python, 233 lines - figures/
fig3.R , R, 362 lines, 1 match - figures/
fig4.R , R, 342 lines, 4 matches - figures/
summary_tables.R , R, 37 lines - pipeline/
ORFPipeline.py , Python, 437 lines, 1 match - pipeline/
ORFPipelineSummed.py , Python, 433 lines - pipeline/
ribobaseFTFilter.py , Python, 226 lines, 1 match - README.md, Text, 114 lines
Code availability statement
The paper has a code availability statement. Its license (CC BY-NC-ND) does not allow reproducing it here; in short, from what the harvester recognized in it:
- it points to the authors' code: up114/
translons
Read it in the paper: doi.org/10.1038/s41467-026-75571-y.
Tracing map
Proposed by the machine: these links were found in the paper and verified at the source, without human review. The map will receive a Zenodo DOI once one of the paper's authors has validated it with their ORCID.
What the map holds:
- 1 repository of the authors' code, each at its verified commit, with its license and how the link was found in the paper;
- 8 scripts, each with its path and the digest of its content;
- 7 matches between paragraphs of the paper and lines of the code (method lexical-v1);
- neither the text of the paper nor the code itself.
Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.
Data
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Data availability statement
The paper has a data availability statement. Its license (CC BY-NC-ND) does not allow reproducing it here; in short, from what the harvester recognized in it:
- no repository, dataset or request procedure was recognized in it
Read it in the paper: doi.org/10.1038/s41467-026-75571-y.
Versions
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Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 6 authors, 2 keywords, 12 MeSH terms, 3 funders, 112 references, 1 RRID.
Cite
This paper
Ghatpande, V., Paul, U., Persyn, L., Tian, Y., Howard, M. A., & Cenik, C. (2026). Ribo-ITP enables identification of translons from limited input samples. Nature communications, 17(1), 9264. https://
BibTeX
@article{ghatpande2026ri
author = {Ghatpande, Vighnesh and Paul, Uma and Persyn, Logan and Tian, Yifan and Howard, MacKenzie A. and Cenik, Can},
title = {{Ribo-ITP enables identification of translons from limited input samples}},
journal = {Nature communications},
year = {2026},
month = jul,
volume = {17},
number = {1},
pages = {9264},
publisher = {Nature Publishing Group},
issn = {2041-1723},
doi = {10.1038/
url = {https://
pmid = {42669660},
pmcid = {PMC13526858}
}
RIS
TY - JOUR
AU - Ghatpande, Vighnesh
AU - Paul, Uma
AU - Persyn, Logan
AU - Tian, Yifan
AU - Howard, MacKenzie A.
AU - Cenik, Can
TI - Ribo-ITP enables identification of translons from limited input samples
T2 - Nature communications
J2 - Nat Commun
PY - 2026
DA - 2026/
VL - 17
IS - 1
SP - 9264
SN - 2041-1723
PB - Nature Publishing Group
DO - 10.1038/
UR - https://
LA - en
ER -
CSL-JSON
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"type": "article-journal",
"title": "Ribo-ITP enables identification of translons from limited input samples",
"container-title": "Nature communications",
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"family": "Ghatpande",
"given": "Vighnesh"
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"issued": {
"date-parts": [
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