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Three-dimensional voltage imaging in live larval zebrafish brains using fully genetically encoded voltage indicator.

Overview

Authors: Eun-Seo Cho1, Minho Eom1, Shihao Zhou2,3,4, Gyuri Kim1, Soi Kim1, Cheol-Hee Kim5, Kiryl D Piatkevich2,3,4, Young-Gyu Yoon1,6,7
ORCID iDs: Eun-Seo Cho
  1. School of Electrical Engineering, KAIST, Daejeon, Republic of Korea
  2. School of Life Sciences, Westlake University, Hangzhou, Zhejiang China
  3. Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang China
  4. Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, Zhejiang China
  5. Department of Biology, Chungnam National University, Daejeon, Republic of Korea
  6. KAIST Institute for Human Augmentation Convergence, Daejeon, Republic of Korea
  7. Department of Semiconductor System Engineering, KAIST, Daejeon, Republic of Korea
Institutions: Korea Advanced Institute of Science and Technology (South Korea); Westlake University (China); Chungnam National University (South Korea)
Journal: Scientific reports, volume 16, issue 1, article 27491
Dates: received 21 March 2026; accepted 11 June 2026; published online 16 June 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1038/s41598-026-58107-8 · PMID 42303690 · PMCID PMC13534597 · OpenAlex W7164897683
Open access: gold, a free copy (OpenAlex)
Status: data only
Categories: optical imaging (calcium, voltage, 2-photon) (modality), zebrafish (organism)
Methods: Preprocessing, fMRI & imaging, Single-unit activity, calcium imaging, Smoothing, state filtering, decompositions, Physiology & signal measures
Keywords: Biological techniques, Biotechnology, Engineering, Neuroscience
MeSH: Brain*, Imaging, Three-Dimensional*, Zebrafish*, Animals, Animals, Genetically Modified, Larva, Membrane Potentials, Neurons (* major topic)
Topic: Planarian Biology and Electrostimulation (Molecular Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: National Research Foundation of Korea (RS-2026-25475246); KAIST C2(Creative & Challenging) Project; National Natural Science Foundation of China (32571589); Human Frontier Science Program (RGP003/2024); Ministry of Science and ICT, South Korea (RS-2024-00401676); KAIST Quantum+X Convergence R&D Project
Citations: not cited yet (Europe PMC); 50 references in the paper

Abstract

Voltage imaging has emerged as a powerful tool for recording membrane potential changes in living cells, offering a direct measurement of rapid neuronal events with high temporal precision. Since the brain is a three-dimensional circuit, it is essential to record signals across a volume. However, achieving effective three-dimensional voltage imaging over large neuronal populations remains challenging due to the need for high imaging speed, high signal-to-noise ratio, and extensive volume coverage. In this study, we demonstrate in vivo three-dimensional voltage imaging in larval zebrafish using oblique plane microscopy and QFDBD-QUAS-driven expression of the genetically encoded voltage indicator Ace-mNeon2-Kv2.1, achieving volumetric imaging rates of up to 200 volumes per second (VPS). This approach enables dye-free voltage imaging, simplifying experimental workflows and improving the reproducibility of in vivo voltage imaging experiments for investigating neuronal circuit dynamics in the living zebrafish animal model.

Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1038/s41598-026-58107-8.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

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Tracing map

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Data

Datasets cited

Data availability

The datasets generated and/or analysed during the current study are available in the Zenodo repository (https://zenodo.org/records/19235146). The plasmid used for the generation of the ElectraOFF zebrafish line and its full sequence are available from the WeKwikGene repository (https://wekwikgene.wllsb.edu.cn/plasmids/0000566). The transgenic zebrafish lines generated in this study will be made available upon reasonable request to the corresponding authors.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 8 authors, 4 keywords, 8 MeSH terms, 6 funders, 47 references.

Cite

This paper

Cho, E.-S., Eom, M., Zhou, S., Kim, G., Kim, S., Kim, C.-H., Piatkevich, K. D., & Yoon, Y.-G. (2026). Three-dimensional voltage imaging in live larval zebrafish brains using fully genetically encoded voltage indicator. Scientific reports, 16(1), 27491. https://doi.org/10.1038/s41598-026-58107-8

BibTeX

@article{cho2026three,
author = {Cho, Eun-Seo and Eom, Minho and Zhou, Shihao and Kim, Gyuri and Kim, Soi and Kim, Cheol-Hee and Piatkevich, Kiryl D and Yoon, Young-Gyu},
title = {{Three-dimensional voltage imaging in live larval zebrafish brains using fully genetically encoded voltage indicator}},
journal = {Scientific reports},
year = {2026},
month = jun,
volume = {16},
number = {1},
pages = {27491},
publisher = {Nature Publishing Group},
issn = {2045-2322},
doi = {10.1038/s41598-026-58107-8},
url = {https://doi.org/10.1038/s41598-026-58107-8},
pmid = {42303690},
pmcid = {PMC13534597}
}

RIS

TY - JOUR
AU - Cho, Eun-Seo
AU - Eom, Minho
AU - Zhou, Shihao
AU - Kim, Gyuri
AU - Kim, Soi
AU - Kim, Cheol-Hee
AU - Piatkevich, Kiryl D
AU - Yoon, Young-Gyu
TI - Three-dimensional voltage imaging in live larval zebrafish brains using fully genetically encoded voltage indicator
T2 - Scientific reports
J2 - Sci Rep
PY - 2026
DA - 2026/06/16
VL - 16
IS - 1
SP - 27491
SN - 2045-2322
PB - Nature Publishing Group
DO - 10.1038/s41598-026-58107-8
UR - https://doi.org/10.1038/s41598-026-58107-8
LA - en
ER -

CSL-JSON

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