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<i>C. elegans</i> somatostatin/allatostatin C signaling regulates sleep, metabolism, survival, and memory via a sleep-active neuron.

Overview

Authors: Byoungjun Park1, Lama Mohsen1, Inka Busack1, Laura Uhlig1, Lorenzo Rossi1, Gill Pollmeier1, Ellen Geens2, Majdulin Nabil Istiban2, Sajal Mandal1, Reshma Dominic Savio1, Isabel Beets2, Attila Stetak3, Henrik Bringmann1,4
ORCID iDs: Byoungjun Park, Inka Busack, Laura Uhlig, Lorenzo Rossi, Gill Pollmeier, Ellen Geens, Majdulin Nabil Istiban, Sajal Mandal, Reshma Dominic Savio, Isabel Beets, Henrik Bringmann
  1. Biotechnology Center and Medical Faculty, Technische Universität Dresden, Am Tatzberg 47/49, 01307 Dresden, Germany
  2. Department of Biology, KU Leuven, Naamsestraat 59, 3000 Leuven, Belgium
  3. Division Cluster Molecular and Cognitive Neurosciences, Universität Basel, Birmannsgasse 8, 4055 Basel, Switzerland
  4. Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany
Institutions: Biotechnologisches Zentrum (Germany); Technische Universität Dresden (Germany); KU Leuven (Belgium); University of Basel (Switzerland)
Journal: Science advances, volume 12, issue 16, article eadv8387
Dates: received 9 January 2025; accepted 17 March 2026; published online 15 April 2026; in print April 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1126/sciadv.adv8387 · PMID 41984956 · PMCID PMC13082328 · OpenAlex W7154452030
Open access: gold, a free copy (OpenAlex)
Status: code verified
Categories: C. elegans (organism)
Methods: Spectral & time-frequency, Evoked potentials, fMRI & imaging, Single-unit activity, calcium imaging, Smoothing, state filtering, decompositions
MeSH: Caenorhabditis elegans*, Caenorhabditis elegans Proteins*, Memory*, Neurons*, Neuropeptides*, Signal Transduction*, Sleep*, Somatostatin*, Animals, Longevity (* major topic)
Topic: Genetics, Aging, and Longevity in Model Organisms (Aging, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: European Research Council (950328)
Citations: cited by 1 paper (Europe PMC); 80 references in the paper

Abstract

Somatostatin/allatostatin C signaling regulates sleep, metabolism, memory, and longevity, but the underlying neuronal mechanisms remain unclear. We investigated the Caenorhabditis elegans somatostatin/allatostatin C ortholog NLP-99 and its receptor NPR-16. We found that the wake-active AIY neurons release NLP-99 to activate NPR-16. NPR-16 is Gi/o coupled and acts cell nonautonomously to activate the sleep-active RIS neuron while acting autonomously in RIS to inhibit calcium activation and the release of the sleep-inducing FLP-11 neuropeptides. During larval arrest, strong NPR-16 expression in RIS causes NLP-99/NPR-16 signaling to inhibit sleep, reducing lipid storage and survival. In well-fed adults, NPR-16 expression is stronger outside of RIS, and NLP-99/NPR-16 signaling is required for RIS activation and sleep while also inhibiting longevity via RIS. Both NLP-99/NPR-16 and RIS/FLP-11 are required for memory consolidation. These results show that NLP-99/NPR-16 regulates physiological processes via control of RIS. A similar somatostatin-mediated regulation of sleep neurons may underlie physiological regulation in other species.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

No file of the authors' code could be read here: it is described below, and read at its source.

datadryad.org/share/ouen3lvphpyfsny9mpc3f1g9gsnbemn0fpmk0reg06y

License: none: the authors keep all their rights
State: the link answers, verified on 29 September 2026
Evidence: the link answers
Software Heritage: not checked
Found in: “Data, code, and materials availability:”
Not found: README, license file, CITATION.cff, environment file, tests, continuous integration, documentation
Availability: 1 check, the latest on 29 September 2026: the link answers (HTTP 200)
  • 29 September 2026: the link answers (HTTP 200)
At the source: datadryad.org/share/Ouen3lVphPyFSnY9mpc3f1G9GSNbemn0fpmk0rEG06Y

The paper's code and data availability statement is in the Data section.

Tracing map

Proposed by the machine: these links were found in the paper and verified at the source, without human review. The map will receive a Zenodo DOI once one of the paper's authors has validated it with their ORCID.

What the map holds:

  • 1 repository of the authors' code, each at its verified commit, with its license and how the link was found in the paper;
  • 0 scripts, each with its path and the digest of its content;
  • no match between paragraphs and code yet;
  • neither the text of the paper nor the code itself.

Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.

Data

No dataset and no data link were found in the paper.

Data, code, and materials availability

Key C. elegans strains used in this study are available from the Caenorhabditis Genetics Center. Additional C. elegans strains and other reagents generated for this study are available from the corresponding author upon reasonable request (). All data and code needed to evaluate and reproduce the results in the paper are present in the paper and/or the Supplementary Materials. Original data and software code are available at Dryad: http://datadryad.org/share/Ouen3lVphPyFSnY9mpc3f1G9GSNbemn0fpmk0rEG06Y.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 29 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 13 authors, 10 MeSH terms, 1 funder, 80 references.

Cite

This paper

Park, B., Mohsen, L., Busack, I., Uhlig, L., Rossi, L., Pollmeier, G., Geens, E., Istiban, M. N., Mandal, S., Savio, R. D., Beets, I., Stetak, A., & Bringmann, H. (2026). <i>C. elegans</i> somatostatin/allatostatin C signaling regulates sleep, metabolism, survival, and memory via a sleep-active neuron. Science advances, 12(16), eadv8387. https://doi.org/10.1126/sciadv.adv8387

BibTeX

@article{park2026lt,
author = {Park, Byoungjun and Mohsen, Lama and Busack, Inka and Uhlig, Laura and Rossi, Lorenzo and Pollmeier, Gill and Geens, Ellen and Istiban, Majdulin Nabil and Mandal, Sajal and Savio, Reshma Dominic and Beets, Isabel and Stetak, Attila and Bringmann, Henrik},
title = {{\<i\>C. elegans\</i\> somatostatin/allatostatin C signaling regulates sleep, metabolism, survival, and memory via a sleep-active neuron}},
journal = {Science advances},
year = {2026},
month = apr,
volume = {12},
number = {16},
pages = {eadv8387},
publisher = {American Association for the Advancement of Science},
issn = {2375-2548},
doi = {10.1126/sciadv.adv8387},
url = {https://doi.org/10.1126/sciadv.adv8387},
pmid = {41984956},
pmcid = {PMC13082328}
}

RIS

TY - JOUR
AU - Park, Byoungjun
AU - Mohsen, Lama
AU - Busack, Inka
AU - Uhlig, Laura
AU - Rossi, Lorenzo
AU - Pollmeier, Gill
AU - Geens, Ellen
AU - Istiban, Majdulin Nabil
AU - Mandal, Sajal
AU - Savio, Reshma Dominic
AU - Beets, Isabel
AU - Stetak, Attila
AU - Bringmann, Henrik
TI - <i>C. elegans</i> somatostatin/allatostatin C signaling regulates sleep, metabolism, survival, and memory via a sleep-active neuron
T2 - Science advances
J2 - Sci Adv
PY - 2026
DA - 2026/04/15
VL - 12
IS - 16
SP - eadv8387
SN - 2375-2548
PB - American Association for the Advancement of Science
DO - 10.1126/sciadv.adv8387
UR - https://doi.org/10.1126/sciadv.adv8387
LA - en
ER -

CSL-JSON

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