Methodological trends in biological assessment of children's daily stress: A scoping review.
Overview
- Department of Human Development & Family Studies, Duksung Women’s University, Seoul, Republic of Korea
- Department of Family and Child Welfare, Wonkwang University, Iksan, Republic of Korea
Abstract
Introduction: Childhood stress can influence biological stress-response systems and may have long-term implications for development. Although biological measures are increasingly used to assess stress in children, research on biological assessments of daily stress among typically developing children has not yet been comprehensively synthesized across stress types and disciplines.
Objective: This scoping review synthesizes research from the past 20 years on biological assessments of daily stress in typically developing children, distinguishing between chronic and acute stress and integrating evidence across medicine, psychology, and education.
Methods: Following established scoping review frameworks, we searched PubMed, PsycArticles, and EBSCO Education Source for English-language observational and experimental studies from 2005 to 2025. Studies assessing daily stress using biological indicators in typically developing children were included. Review articles, case studies, pilot studies, and intervention studies were excluded. Data were charted on publication year, country, child age, stress type, data collection methods, biological indicators, and assessment context.
Results: Fifty-seven studies met the inclusion criteria. Overall, the number of studies increased between 2005 and 2019, followed by a modest decline between 2020 and 2025. Acute stress studies were more prevalent than chronic stress studies. Chronic stress research most frequently involved preschool-aged children, whereas acute stress studies most frequently targeted upper elementary-aged children. Endocrine measures—particularly cortisol—were most frequently used, followed by autonomic nervous system measures, whereas brain- and immune-related markers remained limited. The use of repeated daily cortisol sampling increased markedly between 2015 and 2019, followed by a modest decline in 2020–2025, while remaining above levels observed in earlier periods.
Conclusion: This review maps methodological trends in biological assessments of daily stress in typically developing children and identifies research gaps across stress types, age groups, biological indicators, and cortisol assessment practices. These findings can inform more comprehensive and methodologically robust approaches to understanding children’s daily stress.
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Version 3, 28 September 2026
- Funding: added Ministry of Education: NRF-2024S1A5A8028328; National Research Foundation; National Research Foundation of Korea
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Recorded: type, language, journal, volume, issue, pages, dates, 2 authors, 5 MeSH terms, 69 references.
Cite
This paper
Kang, S., & Kim, E. (2026). Methodological trends in biological assessment of children's daily stress: A scoping review. PloS one, 21(8), e0355460. https://
BibTeX
@article{kang2026methodo
author = {Kang, Sujeong and Kim, Eunkyung},
title = {{Methodological trends in biological assessment of children's daily stress: A scoping review}},
journal = {PloS one},
year = {2026},
month = aug,
volume = {21},
number = {8},
pages = {e0355460},
publisher = {PLOS},
issn = {1932-6203},
doi = {10.1371/
url = {https://
pmid = {42574374},
pmcid = {PMC13456278}
}
RIS
TY - JOUR
AU - Kang, Sujeong
AU - Kim, Eunkyung
TI - Methodological trends in biological assessment of children's daily stress: A scoping review
T2 - PloS one
J2 - PLoS One
PY - 2026
DA - 2026/
VL - 21
IS - 8
SP - e0355460
SN - 1932-6203
PB - PLOS
DO - 10.1371/
UR - https://
LA - en
ER -
CSL-JSON
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