Spiking and neuromodulation during active experience shape visuomotor integration in V1 layer 2/3 neurons

Overview

1. Institute for Neuroscience and Cardiovascular Research, University of Edinburgh, 1 George Square, EH8 9JZ, Edinburgh, United Kingdom
2. Simons Initiative for the Developing Brain

Abstract

Computational models propose that prediction errors drive plasticity to improve cancellation between sensory and predictive inputs - a process known as prediction error minimization. In mouse V1, layer 2/3 neurons exhibit spiking responses consistent with prediction errors between visual flow and locomotion-based predictions, but evidence that such spiking drives plasticity consistent with prediction error minimization is limited. To address this, we performed in vivo whole cell recordings in mice running in virtual reality while manipulating spike rates via closed-loop locomotion-coupled current injection. Spiking during visuomotor experience altered locomotion-related inputs in a firing rate-dependent manner. The direction of change depended on each neuron’s visual responsiveness, increasing the opposition between locomotion-related and visual inputs. Analyzing a published two-photon calcium imaging dataset, we demonstrate that a similar activity-dependent reorganization of visuomotor inputs is evident at the population level, but only when locus coeruleus axons were optogenetically activated during visuomotor experience. Together, our results provide evidence for refinements of prediction error computation in layer 2/3 that are driven by spiking and facilitated by neuromodulation across active experience.

Reproduced under the paper's license (CC BY-NC), from the paper cited above.

Code

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Data

Datasets cited

• zenodo:8006971, at Zenodo; found in “Data and code availability”

Data and code availability

The whole cell recording dataset and analytical code will be made publicly available alongside peer-reviewed publication. The two-photon calcium imaging dataset analyzed here was published previously (Jordan and Keller, 2023) and is publicly available (10.5281/zenodo.8006971 (http://dx.doi.org/10.5281/zenodo.8006971)).

Reproduced under the paper's license (CC BY-NC), from the paper cited above.

Versions

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Version 1, 30 September 2026: the first record

Recorded: type, journal, dates, 3 authors, 1 funder, 38 references.

Cite

This paper

Ye, S. Y., Banqueri, M., & Jordan, R. (2026). Spiking and neuromodulation during active experience shape visuomotor integration in V1 layer 2/3 neurons. bioRxiv (preprint). https://doi.org/10.64898/2026.03.03.705098

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