Neuropeptidergic circuit modulation of developmental sleep in <i>Drosophila</i>.
Paper
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The authors' code
R · 12 lines · 499 B · MIT
- library(ggplot2)
- library(dplyr)
- library(reshape2)
- # params
- thresh = 543 # dPixel>thresh -> "1" (moved) ## Example value; calibrate for each dataset if acquisition conditions differ
- bout.thresh = 12 # in frame ## Example value; calibrate for each dataset if acquisition conditions differ
- nframe = 56517
- nhour = 18
- srate = nframe / nhour
- jet.colors = colorRampPalette(c("darkblue", "blue", "deepskyblue3", "mediumseagreen", "orange", "yellow", "lemonchiffon"))
- ncore = detectCores()[1] - 1
20211129_init.R, under MIT · at the source
Overview
- Department of Biological Sciences, The University of Tokyo, Tokyo, Japan
- International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo, Tokyo, Japan
Abstract
Sleep–wakefulness regulation dynamically evolves along development in a wide range of organisms. While the mechanism regulating sleep in adults is relatively well understood, little is known about its counterpart in early developmental stages. Here, we report a neuropeptidergic circuitry that modulates sleep in developing Drosophila larvae. Through an unbiased screen, we identified the neuropeptide Hugin and its receptor PK2-R1 as critical regulators of larval sleep. Our genetic and behavioral data suggest that HugPC neurons secrete Hugin peptides to activate insulin-producing cells (IPCs), which express a Hugin receptor PK2-R1. IPCs, in turn, release insulin-like peptides (Dilps) to regulate sleep. We further show that the Hugin/
Reproduced under the paper's license (CC BY), from the paper cited above.
Repositories
Its files are read in the Code ↔ Paper reader above.
Zenodo 18996367
Availability: 1 check, the latest on 29 September 2026: the link answers (HTTP 200)
- 29 September 2026: the link answers (HTTP 200)
7 files
- 20211129_init.R — R, 12 lines
- 20211201_data_processing
.R — R, 41 lines - 20211201_functions_data_
processing.R — R, 118 lines - batch_24well_binary-thre
sh_AppliedPC_8.py — Python, 25 lines - videoanalyzer_single_loo
p_ROI_F_binary-thresh_Ap — Python, 163 linespliedPC_8.py - LICENSE — License, 21 lines
- README.md — Text, 28 lines
chikayo2020-2024/drosophila-larval-sleep-code
ca435dfd1d34eebe27177f09147a65040ec3f0fe, 13 March 2026Availability: 1 check, the latest on 29 September 2026: the link answers
- 29 September 2026: the link answers
7 files
- 20211129_init.R — R, 12 lines
- 20211201_data_processing
.R — R, 41 lines - 20211201_functions_data_
processing.R — R, 118 lines - batch_24well_binary-thre
sh_AppliedPC_8.py — Python, 25 lines - videoanalyzer_single_loo
p_ROI_F_binary-thresh_Ap — Python, 163 linespliedPC_8.py - LICENSE — License, 21 lines
- README.md — Text, 28 lines
The paper's code and data availability statement is in the Data section.
Tracing map
Proposed by the machine: these links were found in the paper and verified at the source, without human review. The map will receive a Zenodo DOI once one of the paper's authors has validated it with their ORCID.
What the map holds:
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- 10 scripts, each with its path and the digest of its content;
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Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.
Data
No dataset and no data link were found in the paper.
Data availability
All data necessary to reproduce the figures and statistical analyses are provided as Source data 1 with the manuscript. The source code used in this study is available at https://
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 1, 29 September 2026: the first record
Recorded: type, language, journal, volume, pages, dates, 5 authors, 1 keyword, 9 MeSH terms, 2 funders, 52 references, 38 RRIDs.
Cite
This paper
Hemmi, C., Ishii, K., Motoyoshi, M., Tsuji, M., & Emoto, K. (2026). Neuropeptidergic circuit modulation of developmental sleep in &
BibTeX
@article{hemmi2026neurop
author = {Hemmi, Chikayo and Ishii, Kenichi and Motoyoshi, Mana and Tsuji, Masato and Emoto, Kazuo},
title = {{Neuropeptidergic circuit modulation of developmental sleep in \&
journal = {eLife},
year = {2026},
month = apr,
volume = {14},
pages = {RP105710},
publisher = {eLife Sciences Publications, Ltd},
issn = {2050-084X},
doi = {10.7554/
url = {https://
pmid = {41949906},
pmcid = {PMC13061416}
}
RIS
TY - JOUR
AU - Hemmi, Chikayo
AU - Ishii, Kenichi
AU - Motoyoshi, Mana
AU - Tsuji, Masato
AU - Emoto, Kazuo
TI - Neuropeptidergic circuit modulation of developmental sleep in &
T2 - eLife
J2 - eLife
PY - 2026
DA - 2026/
VL - 14
SP - RP105710
SN - 2050-084X
PB - eLife Sciences Publications, Ltd
DO - 10.7554/
UR - https://
LA - en
ER -
CSL-JSON
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"family": "Tsuji",
"given": "Masato"
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{
"family": "Emoto",
"given": "Kazuo"
}
],
"container-title-short":
"volume": "14",
"page": "RP105710",
"DOI": "10.7554/
"PMID": "41949906",
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"ISSN": "2050-084X",
"publisher": "eLife Sciences Publications, Ltd",
"URL": "https://
"language": "en",
"issued": {
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}
}
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