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Oxytocin neurons signal state-dependent transitions from rest to thermogenesis and behavioral arousal.

Code ↔ Paper

7 matches between paragraphs of the paper and lines of its authors' code, computed by the harvester (lexical-v1). Click a colored paragraph or line to see its counterpart.

The 7 matches
  1. [1] § Materials and methods › Computer vision analysis › SGBS for thermographic temperature extraction ↔ src/utils/utils.py, lines 204 to 301 · score 0.79 · predicted box, bounding box, predicted mask, temperature statistics, class, min · Report a mismatch
  2. [2] § Materials and methods › Fiber photometry recordings › Calcium data preprocessing ↔ Code/NP_processed_homecage_v8_lactation.R, lines 1196 to 1248 · score 0.79 · baseline subtracted, half max, pre peak, peak normalized, trace, window · Report a mismatch
  3. [3] § Materials and methods › Computer vision analysis › SGBS for thermographic temperature extraction ↔ src/data/Data_alignment.py, lines 368 to 446 · score 0.69 · ground truth, bounding box, skeleton, mapped, min, training · Report a mismatch
  4. [4] § Materials and methods › Computer vision analysis › SGBS for thermographic temperature extraction ↔ src/models/SG_MaskRCNN_train.py, lines 85 to 118 · score 0.68 · CNN model, custom Mask, network, trained, channels, skeleton · Report a mismatch
  5. [5] § Results › PVNOT peaks signal increases in BAT thermogenesis and changes in regional surface temperature ↔ src/models/SG_MaskRCNN_train.py, lines 85 to 118 · score 0.63 · attention layer, skeleton guided, network, CNN, trained, keypoints · Report a mismatch
  6. [6] § Results › PVNOT Ca2+ peaks track rest and arousal behavior states in social and non-social conditions ↔ Code/NP_processed_homecage_v8.R, lines 1967 to 2045 · score 0.57 · bout duration, bout length, active huddling, ambient, nesting, solo · Report a mismatch
  7. [7] § Materials and methods › Statistics › Effect of optogenetic stimulation on thermal profile ↔ Code/Opto_processed_v3.R, lines 624 to 673 · score 0.54 · LightOn, core Tb, mouse ID, bins, stimulus, 60 s · Report a mismatch

Paper

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The authors' code

Python · 240 lines · 10 KB · MIT · 2 matches

  1. import os
  2. import torch
  3. import torch.nn as nn
  4. import torchvision
  5. import json
  6. from torchvision.models import resnet50, ResNet50_Weights
  7. from torchvision.models.detection import MaskRCNN
  8. from torchvision.models.detection.rpn import AnchorGenerator
  9. from src.data.Data_alignment import prepare_dataloader
  10. from src.utils.utils import keypoints_to_heatmaps, visualize_prediction_images, filter_duplicate_classes
  11. import csv
  12. os.environ["KMP_DUPLICATE_LIB_OK"] = "TRUE" # Ignore
  13. # Define directories
  14. csv_directory = r'F:\DLC_thermography-JLanden-2024-07-09\videos' #Keypoints from DLC
  15. image_directory = r'F:\DLC_thermography-JLanden-2024-07-09\labeled-data' # Images
  16. segments_dirs = r'F:\Segmentation\training_segments' # Training segments from Darwin
  17. save_to_dir = r'F:\Segmentation\compare_02-13-25_BS8_check' #Where output is saved
  18. # Parameters
  19. num_epochs = 200
  20. validate_every_n_epochs = 200
  21. batch_size = 8 # Adjust as needed
  22. val_split = 0.2 # 20% of the data for validation
  23. #mask_threshold = 0.5 # 0.5 is normal
  24. mask_display_threshold = 0.9
  25. save_weights = False
  26. save_losses = True
  27. image_size = (348, 464) # H, W
  28. num_keypoints = 19
  29. num_classes = 4 # Set this according to your dataset (# classes + background)
  30. class_names = ['background', 'implanted_mouse', 'implanted_bat', 'implanted_rump' #,
  31. # , 'extra_mouse', 'extra_bat', 'extra_rump'
  32. ]
  33. loss_history = [] # Store loss values for CSV
  34. colors = [(1, 0, 0), (.1, .6, .1), (0, 0, 1)] #R, G, B: Must match number of class names
  35. # Attention Mechanism
  36. class AttentionLayer(nn.Module):
  37. def __init__(self, img_channels, keypoint_channels, attention_channels):
  38. super(AttentionLayer, self).__init__()
  39. # Convolution layers for image and keypoints (input)
  40. self.img_conv1 = nn.Conv2d(img_channels, attention_channels, kernel_size=1)
  41. self.img_conv2 = nn.Conv2d(attention_channels, attention_channels, kernel_size=3, padding=1)
  42. self.img_conv3 = nn.Conv2d(attention_channels, attention_channels, kernel_size=3, padding=1)
  43. self.keypoint_conv1 = nn.Conv2d(keypoint_channels, attention_channels, kernel_size=1)
  44. self.keypoint_conv2 = nn.Conv2d(attention_channels, attention_channels, kernel_size=3, padding=1)
  45. self.keypoint_conv3 = nn.Conv2d(attention_channels, attention_channels, kernel_size=3, padding=1)
  46. # Activation function
  47. self.relu = nn.ReLU()
  48. # Convolution for the attention mechanism (combine image and keypoint features)
  49. self.attention_conv1 = nn.Conv2d(attention_channels, attention_channels, kernel_size=3, padding=1)
  50. self.attention_conv2 = nn.Conv2d(attention_channels, attention_channels, kernel_size=3, padding=1)
  51. self.sigmoid = nn.Sigmoid()
  52. def forward(self, images, keypoints):
  53. # Process the image through multiple convolutions
  54. img_out = self.relu(self.img_conv1(images))
  55. img_out = self.relu(self.img_conv2(img_out))
  56. img_out = self.relu(self.img_conv3(img_out))
  57. # Process the keypoints through multiple convolutions
  58. keypoint_out = self.relu(self.keypoint_conv1(keypoints))
  59. keypoint_out = self.relu(self.keypoint_conv2(keypoint_out))
  60. keypoint_out = self.relu(self.keypoint_conv3(keypoint_out))
  61. # Combine image and keypoint features using element-wise multiplication
  62. combined = img_out * keypoint_out
  63. # Apply further attention convolutions
  64. attention_map = self.relu(self.attention_conv1(combined))
  65. attention_map = self.sigmoid(self.attention_conv2(attention_map))
  66. # Apply attention to image features
  67. enhanced_img_features = images * attention_map
  68. return enhanced_img_features
  69. # Custom Mask R-CNN Model with Attention
  70. class SkeletonGuidedMaskRCNN(nn.Module):
  71. def __init__(self, num_classes):
  72. super(SkeletonGuidedMaskRCNN, self).__init__()
  73. # Attention layer first to overlay images with keypoints
  74. self.attention = AttentionLayer(img_channels=3, keypoint_channels=num_keypoints, attention_channels=3)
  75. # Load a pre-trained Mask R-CNN backbone
  76. backbone = resnet50(weights=ResNet50_Weights.IMAGENET1K_V2)
  77. backbone = nn.Sequential(*list(backbone.children())[:-2]) # Remove last fully connected layer
  78. self.backbone = backbone
  79. self.backbone.out_channels = 2048
  80. # Create an Anchor Generator for the FPN (feature pyramid network)
  81. anchor_generator = AnchorGenerator(sizes=((32, 64, 128, 256, 512),), aspect_ratios=((0.5, 1.0, 2.0),) * 5)
  82. # Define RoI Align and FPN
  83. roi_pooler = torchvision.ops.MultiScaleRoIAlign(featmap_names=['0'], output_size=4, sampling_ratio=2)
  84. # Define Mask R-CNN model with the modified backbone
  85. self.model = MaskRCNN(self.backbone, num_classes=num_classes, rpn_anchor_generator=anchor_generator,
  86. box_roi_pool=roi_pooler, mask_roi_pool=roi_pooler)
  87. def forward(self, images, keypoints, targets=None, return_predictions=False):
  88. # Apply attention to images using keypoints
  89. enhanced_img_features = self.attention(images, keypoints)
  90. # Use enhanced image features in the Mask R-CNN model
  91. if self.training and not return_predictions:
  92. return self.model(enhanced_img_features, targets)
  93. else: # Validate
  94. return self.model(enhanced_img_features)
  95. # Training script
  96. # Initialize the custom loss
  97. # loss_fn = CustomLoss()
  98. ########################
  99. def save_predictions_to_json(predictions, save_to_dir, videoID, frameID):
  100. os.makedirs(f"{save_to_dir}/predictions/{videoID}", exist_ok=True)
  101. json_path = os.path.join(save_to_dir, f"predictions", videoID, f"predictions_{frameID}.txt")
  102. serializable_predictions = []
  103. for pred in predictions:
  104. serializable_predictions.append({
  105. 'labels': pred['labels'].cpu().tolist(), # Convert tensor to list
  106. 'scores': pred['scores'].cpu().tolist(), # Convert tensor to list
  107. 'boxes': pred['boxes'].cpu().tolist(), # Convert tensor to list
  108. 'masks': pred['masks'].cpu().numpy().tolist() # Convert tensor to nested lists
  109. })
  110. # Write the predictions to a file in JSON format
  111. with open(json_path, 'w') as f:
  112. json.dump(serializable_predictions, f, indent=4)
  113. #####################
  114. def train_model(train_loader, val_loader, num_classes, num_epochs=num_epochs):
  115. device = torch.device("cuda") if torch.cuda.is_available() else torch.device("cpu")
  116. model = SkeletonGuidedMaskRCNN(num_classes=num_classes).to(device)
  117. optimizer = torch.optim.Adam(model.parameters(), lr=1e-4)
  118. for epoch in range(num_epochs):
  119. model.train()
  120. total_loss = 0
  121. for batch_idx, (images, segments, skeletons) in enumerate(train_loader):
  122. images = [image.to(device) for image in images]
  123. H, W = images[0].shape[1], images[0].shape[2]
  124. # Convert images to tensors
  125. images = torch.stack([image.to(device) for image in images]).to(device)
  126. keypoints = skeletons.to(device)
  127. kp_heatmaps = keypoints_to_heatmaps(keypoints, H, W)
  128. segments = [{k: (v.to(device) if isinstance(v, torch.Tensor) else v) for k, v in segment.items()} for
  129. segment in segments]
  130. optimizer.zero_grad()
  131. outputs = model(images, kp_heatmaps, segments)
  132. # Use the losses from the model's output
  133. rcnn_losses = sum(loss for loss in outputs.values())
  134. total_loss = rcnn_losses
  135. total_loss.backward()
  136. optimizer.step()
  137. total_loss += total_loss.item()
  138. avg_loss = total_loss / len(train_loader)
  139. print(f"Epoch [{epoch + 1}/{num_epochs}], Loss: {avg_loss}")
  140. # Store loss history for CSV
  141. loss_history.append([epoch + 1, avg_loss.item()])
  142. if (epoch + 1) % validate_every_n_epochs == 0:
  143. model.eval()
  144. predictions_list = []
  145. for batch_idx, (images, segments, skeletons) in enumerate(val_loader):
  146. images = [image.to(device) for image in images]
  147. H, W = images[0].shape[1], images[0].shape[2]
  148. images = torch.stack([image.to(device) for image in images]).to(device)
  149. keypoints = skeletons.to(device)
  150. kp_heatmaps = keypoints_to_heatmaps(keypoints, H, W)
  151. segments = [{k: (v.to(device) if isinstance(v, torch.Tensor) else v) for k, v in segment.items()} for
  152. segment in segments]
  153. with torch.no_grad(): # Disable gradient calculation
  154. # Get predictions instead of losses
  155. predictions = model(images, kp_heatmaps)
  156. predictions = filter_duplicate_classes(predictions)
  157. predictions_list.append(predictions)
  158. # Optionally visualize here
  159. visualize_prediction_images(
  160. images=images,
  161. predictions=predictions,
  162. epoch=epoch + 1,
  163. phase="validate",
  164. batch_idx=batch_idx,
  165. save_img_dir=save_to_dir,
  166. class_names=class_names,
  167. colors=colors,
  168. keypoints=keypoints,
  169. threshold=mask_display_threshold
  170. )
  171. else:
  172. continue
  173. # Save model weights
  174. if save_weights is True:
  175. model_save_path = os.path.join(save_to_dir, f'sg-mrcnn_epochs_{num_epochs}.pth')
  176. torch.save(model.state_dict(), model_save_path)
  177. print(f"Model weights saved to {model_save_path}")
  178. if save_losses is True:
  179. loss_csv_path = os.path.join(save_to_dir, f"main_loss_{num_epochs}.csv")
  180. # Save loss history to CSV
  181. with open(loss_csv_path, mode="w", newline="") as file:
  182. writer = csv.writer(file)
  183. writer.writerow(["Epoch", "Loss"]) # Write header
  184. writer.writerows(loss_history) # Write epoch, loss pairs
  185. return model
  186. if __name__ == "__main__":
  187. # Assuming train_loader and val_loader are already created with your data
  188. train_loader, val_loader = prepare_dataloader(csv_directory, segments_dirs, image_directory,
  189. batch_size, val_split) # Implement this function
  190. trained_model = train_model(train_loader, val_loader, num_classes)

SG_MaskRCNN_train.py at commit 06d57d8, under MIT · at the source

Overview

Authors: Morgane Vandendoren1, Jason G Landen1, Joseph F Rogers1, Samantha Killmer1, Baizar Alamiri1, Celeste Pohlman1, Glenn Tattersall2, Nicole L Bedford1, Adam Nelson1
  1. Department of Zoology and Physiology, University of Wyoming, Laramie, United States
  2. Department of Biological Sciences, Brock University, St. Catherines, Canada
Institutions: Creighton University (United States); University of Wyoming (United States); Brock University (Canada)
Journal: eLife, volume 14, article RP108212
Dates: published online 30 October 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.7554/elife.108212 · PMID 42960829 · PMCID PMC13722414 · OpenAlex W4414785549
Open access: gold, a free copy (OpenAlex)
Status: code verified (how the code is found and verified)
Categories: mouse (organism)
Methods: Spectral & time-frequency, Machine learning, Statistics, Preprocessing, Evoked potentials, fMRI & imaging, Single-unit activity, calcium imaging, Smoothing, state filtering, decompositions, Connectivity
Keywords: Mouse
MeSH: Arousal*, Behavior, Animal*, Neurons*, Oxytocin*, Paraventricular Hypothalamic Nucleus*, Rest*, Thermogenesis*, Animals, Body Temperature Regulation, Male, Mice (* major topic)
Topic: Neuroendocrine regulation and behavior (Endocrine and Autonomic Systems, Neuroscience), according to OpenAlex
Funding: NIH HHS (5P20GM121310-07); National Science Foundation (2437733)
Citations: not cited yet (Europe PMC); 102 references in the paper

Abstract

Core body temperature (Tb) is defended within narrow limits through thermoregulatory behaviors like huddling, nesting, and physical activity, as well as autonomic responses like brown fat thermogenesis. While Tb displays regulated fluctuations across different behavioral states and rest/arousal cycles, the neural control of these transitions remains poorly understood. Here, we investigate the relationship between oxytocin neurons of the paraventricular hypothalamus (PVNOT) and behavioral and autonomic thermoeffector pathways across physiological states in mice. First, we show that PVNOT neurons are activated during social thermoregulation. We then demonstrate that in vivo PVNOT calcium dynamics align with transitions from rest to thermogenesis and behavioral arousal. Counter to our initial hypothesis, these dynamics were observed in both social and non-social contexts. Using a computer vision model to track thermoeffector pathways, we demonstrate that precisely timed stimulation of PVNOT neurons during low-Tb resting states increases thermogenesis followed by behavioral arousal. We therefore suggest a model in which PVNOT neurons facilitate homeostatic state-dependent transitions in thermo-behavioral states.

Reproduced under the paper's license (CC BY), from the paper cited above.

Repositories

Its files are read in the Code ↔ Paper reader above, with 7 matches between paragraphs and lines of code.

Zenodo 2652896

License: other-open
State: the link answers, verified on 31 October 2026
Evidence: files inventoried
Size: 1 file
Software Heritage: not checked
Found in: the references
Not found: README, license file, CITATION.cff, environment file, tests, continuous integration, documentation
Availability: 1 check, the latest on 31 October 2026: the link answers (HTTP 200)
  • 31 October 2026: the link answers (HTTP 200)
3 files
At the source:

j-landen/seq_process

License: MIT
State: the link answers, verified on 31 October 2026
Evidence: files inventoried
Commit: 90225182607b2faa19f9dacb0252430df0e7d598, 28 October 2026
Languages: Python (1)
Size: 3 files, 1 script
Software Heritage: archived
Found in: the text, “Processing FLIR.seq files”
Holds: README, license file
Not found: CITATION.cff, environment file, tests, continuous integration, documentation
Tools: imageio (1 file), NumPy (1 file), OpenCV (1 file), PyTorch (1 file)
Availability: 1 check, the latest on 31 October 2026: the link answers
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3 files

j-landen/SGBS

License: MIT
State: the link answers, verified on 31 October 2026
Evidence: files inventoried
Commit: 06d57d84fa17d1db45ecec70b686c2c0761716f9, 28 October 2026
Languages: Python (6)
Size: 33 files, 6 scripts
Software Heritage: not archived
Found in: the text, “SGBS for thermographic temperature extraction”
Holds: README, license file, environment (requirements.txt)
Not found: CITATION.cff, tests, continuous integration, documentation
Tools: PyTorch (4 files), Matplotlib (3 files), NumPy (3 files), pandas (2 files), Pillow (2 files), DeepLabCut (1 file), imageio (1 file), scikit-image (1 file)
Availability: 1 check, the latest on 31 October 2026: the link answers
  • 31 October 2026: the link answers
8 files

OSF x9kcj

License: none: the authors keep all their rights
State: the link answers, verified on 31 October 2026
Evidence: files inventoried
Languages: R (3)
Size: 136 files, 3 scripts
Software Heritage: not checked
Found in: “Data availability”
Holds: README
Not found: license file, CITATION.cff, environment file, tests, continuous integration, documentation
Tools: broom (3 files), data.table (3 files), emmeans (3 files), lme4 (3 files), lmerTest (3 files), Plotly (3 files), tidyverse (3 files), cowplot (2 files), ggplot2 (2 files), ggpubr (2 files), multcomp (1 file), pROC (1 file)
Availability: 1 check, the latest on 31 October 2026: the link answers (HTTP 200)
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At the source: osf.io/x9kcj

gtatters/thermimagej

License: MIT
State: the link answers, verified on 31 October 2026
Evidence: files inventoried
Commit: cb82c240d409700460cdbb89df104aa104df18d8, 27 June 2026
Languages: Perl (4)
Size: 41 files, 4 scripts
Software Heritage: not archived
Found in: the Zenodo archive record
Holds: README, license file
Not found: CITATION.cff, environment file, tests, continuous integration, documentation
Availability: 1 check, the latest on 31 October 2026: the link answers
  • 31 October 2026: the link answers
6 files

The paper's code and data availability statement is in the Data section.

Tracing map

Proposed by the machine: these links were found in the paper and verified at the source, without human review. The map will receive a Zenodo DOI once one of the paper's authors has validated it with their ORCID.

What the map holds:

  • 5 repositories of the authors' code, each at its verified commit, with its license and how the link was found in the paper;
  • 15 scripts, each with its path and the digest of its content;
  • 7 matches between paragraphs of the paper and lines of the code (method lexical-v1);
  • neither the text of the paper nor the code itself.

Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.

Data

No dataset and no data link were found in the paper.

Data availability

Data and code underlying this study are publicly available via the Open Science Framework (OSF) at https://osf.io/x9kcj. The repository includes the experimental datasets associated with the main fiber photometry experiments, lactation study, optogenetic experiments, and body temperature-linked photometry experiments, together with metadata files describing session- and animal-level information. Code used for data processing and analysis is also available at this repository.

The following dataset was generated:

Nelson A. 2025. Oxytocin Neurons Signal State-Dependent Transitions From Rest To Thermogenesis And Behavioral Arousal. Open Science Framework. x9kcj

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 31 October 2026: the first record

Recorded: type, language, journal, volume, pages, dates, 9 authors, 1 keyword, 11 MeSH terms, 2 funders, 98 references, 7 RRIDs.

Cite

This paper

Vandendoren, M., Landen, J. G., Rogers, J. F., Killmer, S., Alamiri, B., Pohlman, C., Tattersall, G., Bedford, N. L., & Nelson, A. (2026). Oxytocin neurons signal state-dependent transitions from rest to thermogenesis and behavioral arousal. eLife, 14, RP108212. https://doi.org/10.7554/elife.108212

BibTeX

@article{vandendoren2026oxytocin,
author = {Vandendoren, Morgane and Landen, Jason G and Rogers, Joseph F and Killmer, Samantha and Alamiri, Baizar and Pohlman, Celeste and Tattersall, Glenn and Bedford, Nicole L and Nelson, Adam},
title = {{Oxytocin neurons signal state-dependent transitions from rest to thermogenesis and behavioral arousal}},
journal = {eLife},
year = {2026},
month = oct,
volume = {14},
pages = {RP108212},
publisher = {eLife Sciences Publications, Ltd},
issn = {2050-084X},
doi = {10.7554/elife.108212},
url = {https://doi.org/10.7554/elife.108212},
pmid = {42960829},
pmcid = {PMC13722414}
}

RIS

TY - JOUR
AU - Vandendoren, Morgane
AU - Landen, Jason G
AU - Rogers, Joseph F
AU - Killmer, Samantha
AU - Alamiri, Baizar
AU - Pohlman, Celeste
AU - Tattersall, Glenn
AU - Bedford, Nicole L
AU - Nelson, Adam
TI - Oxytocin neurons signal state-dependent transitions from rest to thermogenesis and behavioral arousal
T2 - eLife
J2 - eLife
PY - 2026
DA - 2026/10/30
VL - 14
SP - RP108212
SN - 2050-084X
PB - eLife Sciences Publications, Ltd
DO - 10.7554/elife.108212
UR - https://doi.org/10.7554/elife.108212
LA - en
ER -

CSL-JSON

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"type": "article-journal",
"title": "Oxytocin neurons signal state-dependent transitions from rest to thermogenesis and behavioral arousal",
"container-title": "eLife",
"author": [
{
"family": "Vandendoren",
"given": "Morgane"
},
{
"family": "Landen",
"given": "Jason G"
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{
"family": "Rogers",
"given": "Joseph F"
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{
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{
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{
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"given": "Celeste"
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{
"family": "Tattersall",
"given": "Glenn"
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"given": "Nicole L"
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"given": "Adam"
}
],
"container-title-short": "eLife",
"volume": "14",
"page": "RP108212",
"DOI": "10.7554/elife.108212",
"PMID": "42960829",
"PMCID": "PMC13722414",
"ISSN": "2050-084X",
"publisher": "eLife Sciences Publications, Ltd",
"URL": "https://doi.org/10.7554/elife.108212",
"language": "en",
"issued": {
"date-parts": [
[
2026,
10,
30
]
]
}
}

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[9] doi:10.1016/j.xcrm.2026.102682 [code]
TET CpG sequence-context-specific DNA demethylation shapes progression of IDH-mutant gliomas.
Journal: Cell reports. Medicine
In common: multcomp, pROC, broom, 8 other tools
[10] doi:10.1016/j.celrep.2023.113305 [code]
High-calorie diets uncouple hypothalamic oxytocin neurons from a gut-to-brain satiation pathway via κ-opioid signaling.
Journal: Cell reports
In common: ggplot2, tidyverse, pandas, 2 other tools, mouse, 6 references

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