Structural and functional brain asymmetry in relation to heterogeneous causes of situs inversus totalis.

Overview

1. Language & Genetics Department, Max Planck Institute for Psycholinguistics,Nijmegen, The Netherlands
2. Donders Institute for Brain, Cognition and Behaviour, Radboud University,Nijmegen, The Netherlands
3. Department of Experimental Clinical and Health Psychology, Ghent University,Ghent, Belgium
4. Department of Medical Neuroscience, Radboud University Medical Center,Nijmegen, The Netherlands

Abstract

Various aspects of brain organization differ between the left and right hemispheres. Clues to the developmental origins of these asymmetries may be gained through associations with situs inversus totalis (SIT), a rare condition in which visceral organs are reversed on the left-right axis. In the largest previous brain imaging analyses of SIT (38 cases, 38 controls from Belgium), typical functional asymmetries such as left-hemisphere language dominance were mostly unaltered, but various aspects of asymmetrical cerebral structure - petalia, bending, and posterior venous anatomy—were often reversed in this condition. SIT can be a monogenic trait that arises from rare genetic variants, usually affecting motile cilia which help to create the embryonic left-right body axis. However, most SIT cases do not have obvious genetic causes and may arise from environmental or random effects during embryogenesis. We sequenced the genomes of 23 SIT cases and 23 controls from the Belgian brain imaging dataset and pooled with prior data from 15 cases and 15 controls. We aimed to discover whether there are altered brain asymmetries in SIT cases with disruptive DNA variants in ciliary genes, or in other types of genes, as compared to genetically unsolved cases. In total, 19 cases had likely causal variants affecting ciliary function, while 19 cases remained genetically unsolved. Functional and structural brain asymmetries were not significantly different in genetically solved versus unsolved SIT cases. Therefore, functional brain asymmetries seem largely independent of known mechanisms of visceral situs formation, while structural brain torque is altered in SIT regardless of the presence or absence of overt genetic causes.

Supplementary Information: The online version contains supplementary material available at 10.1007/s00429-026-03098-5.

Reproduced under the paper's license (CC BY), from the paper cited above.

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Data

Datasets cited

• osf:w48z2, at OSF; found in “Data availability”

Data availability

The genome sequence data cannot be freely available due to privacy/identifiability concerns and the fact that consent was not given for such dissemination. Please contact the corresponding author regarding data access. The brain imaging data are not publicly available due to privacy regulations but are available upon reasonable request. The demographic and image-derived metrics analyzed in the present study are available for download from [https://osf.io/w48z2/?view_only=4d527d7bac13436b9989ecb85ebeebb6](https:/osf.io/w48z2/?view_only=4d527d7bac13436b9989ecb85ebeebb6) and [https://link.springer.com/article/10.1007/s00429-017-1598-5](https:/link.springer.com/article/10.1007/s00429-017-1598-5). Code used in the present study can be accessed here: [https://github.com/MengYunWang/Situs_inversus](https:/github.com/MengYunWang/Situs_inversus).

Reproduced under the paper's license (CC BY), from the paper cited above.

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Version 1, 30 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 6 authors, 7 keywords, 7 MeSH terms, 1 funder, 98 references.

Cite

This paper

Wang, M.-Y., Ng, N. Y. T., Eising, E., Fisher, S. E., Vingerhoets, G., & Francks, C. (2026). Structural and functional brain asymmetry in relation to heterogeneous causes of situs inversus totalis. Brain structure & function, 231(3), 38. https://doi.org/10.1007/s00429-026-03098-5

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