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Circuit organization and transcriptomic heterogeneity of sympathetic circuits innervating cranial structures.

Overview

Authors: Gokhan Senturk1, Shawn Driscoll1, Peter J Osseward II1, Miriam Gullo1, Karen Lettieri1, Samuel L Pfaff1,2
ORCID iDs: Samuel L Pfaff
  1. Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines, La Jolla, CA 92037, USA
  2. Lead contact
Institutions: Salk Institute for Biological Studies (United States)
Journal: Cell reports, volume 45, issue 6, article 117446
Dates: published online 3 June 2026; in print 23 June 2026
Type: Research article · Language: English
License: CC BY-NC
Identifiers: DOI 10.1016/j.celrep.2026.117446 · PMID 42241285 · PMCID PMC13372007 · OpenAlex W7163343616
Open access: gold, a free copy (OpenAlex)
Status: code verified
Categories: genetics / omics (modality), mouse (organism), systems (subfield)
Methods: Connectivity, Statistics, Smoothing, state filtering, decompositions, Machine learning
Keywords: Sympathetic nervous system, Superior cervical ganglion, Scg, Cp: Neuroscience, Cell Type Characterization, Circuit Projection Pattern, Developmental Patterns Of Transcriptomic Types
MeSH: Skull*, Superior Cervical Ganglion*, Sympathetic Nervous System*, Transcriptome*, Animals, Mice, Neurons (* major topic)
Topic: Neuroscience of respiration and sleep (Endocrine and Autonomic Systems, Neuroscience), according to OpenAlex
Funding: National Institute of Neurological Disorders and Stroke (R01 NS135055, R01 NS123160); NINDS NIH HHS (R01 NS135055, R01 NS123160, R01 NS140568, U19 NS112959); Sol Goldman Charitable Trust (P30 014195)
Citations: not cited yet (Europe PMC); 54 references in the paper
Research resources: sheep anti-TH RRID:AB_10002491, goat anti-CTB RRID:AB_10013220, RRID:AB_162543, RRID:AB_2535792, RRID:AB_2535794, RRID:AB_2535865, rabbit anti-TH RRID:AB_390204, rat anti-CD31 RRID:AB_393571, rabbit anti-NPY RRID:AB_518504

Abstract

The superior cervical ganglion (SCG) innervates multiple effector organs within cranial tissues and elicits responses including pupil dilation, piloerection, vasoconstriction, and inhibition of salivation. Coordinated activation of these targets is associated with the display of different emotions; however, the underlying circuit organization and cellular heterogeneity of SCG neurons remain unclear. Here, we combined neuronal tracing with single-cell and spatial transcriptomics to characterize the SCG circuitry and heterogeneity. We found that each SCG neuron innervates a single effector organ. SCG subtypes defined by their projection (P) targets form two major compartments within the ganglia, but individual P-types were intermingled. Mature SCG transcriptomic (T) types emerge postnatally and exhibit rostra-caudal biases. While some T-types were enriched in SCG populations with specific axon projections, they did not show a strict one-to-one correspondence with P-types. These results suggest that individual sympathetic cranial effectors mediating facial emotions are controlled combinatorially by multiple transcriptomic SCG types.

Reproduced under the paper's license (CC BY-NC), from the paper cited above.

Code

No file of the authors' code could be read here: it is described below, and read at its source.

ncbi.nlm.nih.gov/geo/query

License: none: the authors keep all their rights
State: the link answers, verified on 27 September 2026
Evidence: the link answers
Software Heritage: not checked
Found in: the text, “Footnotes”
Not found: README, license file, CITATION.cff, environment file, tests, continuous integration, documentation
Availability: 1 check, the latest on 27 September 2026: the link answers (HTTP 200)
  • 27 September 2026: the link answers (HTTP 200)
At the source: ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325797

Tracing map

Proposed by the machine: these links were found in the paper and verified at the source, without human review. The map will receive a Zenodo DOI once one of the paper's authors has validated it with their ORCID.

What the map holds:

  • 1 repository of the authors' code, each at its verified commit, with its license and how the link was found in the paper;
  • 0 scripts, each with its path and the digest of its content;
  • no match between paragraphs and code yet;
  • neither the text of the paper nor the code itself.

Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.

Data

No dataset and no data link were found in the paper.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 2, 28 September 2026

  • Publisher: — → Cell Press
  • Authors: added Samuel L Pfaff (0000-0002-2142-166X); removed Samuel L Pfaff

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 6 authors, 7 keywords, 7 MeSH terms, 3 funders, 52 references, 9 RRIDs.

Cite

This paper

Senturk, G., Driscoll, S., Osseward, P. J., Gullo, M., Lettieri, K., & Pfaff, S. L. (2026). Circuit organization and transcriptomic heterogeneity of sympathetic circuits innervating cranial structures. Cell reports, 45(6), 117446. https://doi.org/10.1016/j.celrep.2026.117446

BibTeX

@article{senturk2026circuit,
author = {Senturk, Gokhan and Driscoll, Shawn and Osseward, Peter J and Gullo, Miriam and Lettieri, Karen and Pfaff, Samuel L},
title = {{Circuit organization and transcriptomic heterogeneity of sympathetic circuits innervating cranial structures}},
journal = {Cell reports},
year = {2026},
month = jun,
volume = {45},
number = {6},
pages = {117446},
publisher = {Cell Press},
issn = {2211-1247},
doi = {10.1016/j.celrep.2026.117446},
url = {https://doi.org/10.1016/j.celrep.2026.117446},
pmid = {42241285},
pmcid = {PMC13372007}
}

RIS

TY - JOUR
AU - Senturk, Gokhan
AU - Driscoll, Shawn
AU - Osseward, Peter J
AU - Gullo, Miriam
AU - Lettieri, Karen
AU - Pfaff, Samuel L
TI - Circuit organization and transcriptomic heterogeneity of sympathetic circuits innervating cranial structures
T2 - Cell reports
J2 - Cell Rep
PY - 2026
DA - 2026/06/03
VL - 45
IS - 6
SP - 117446
SN - 2211-1247
PB - Cell Press
DO - 10.1016/j.celrep.2026.117446
UR - https://doi.org/10.1016/j.celrep.2026.117446
LA - en
ER -

CSL-JSON

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