Development of a human iPSC-derived corticospinal tract-on-a-chip.
Overview
- Department of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA
- Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA
Abstract
Degeneration of the corticospinal tract is a feature in several neurodegenerative disorders and leads to disability. However, modeling corticospinal neuron (CSN) pathology and corticospinal connectivity is challenging, as there are interspecies differences in these networks. We developed a human induced pluripotent stem cell (hiPSC)-based microfluidic platform for modeling human CSN and spinal motor neuron (SpMN) connectivity. The incorporation of regionally specific astrocyte subtypes (cortical and spinal) in addition to CSNs and SpMNs allows for the modeling of neural cell interactions. Multielectrode array electrophysiology reveals the temporal maturation of the network. Retrograde labeling demonstrates synaptic connectivity between CSNs and SpMN. Optogenetic strategies to selectively activate excitatory cortical neurons (CNs) attenuated by glutamate receptor antagonism confirm the functional relevance of the model. Incorporating morphological, electrophysiological, and physiological measures of corticospinal connectivity, this platform is a versatile model for neurodegenerative disease research and the future development of targeted CSN therapies.
Reproduced under the paper's license (CC BY), from the paper cited above.
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Versions
The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.
Version 2, 28 September 2026
- Authors: added Nicholas John Maragakis (0000-0002-7311-9614); removed Nicholas John Maragakis
Version 1, 28 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 14 authors, 4 keywords, 6 MeSH terms, 5 funders, 75 references.
Cite
This paper
Charalampopoulou, A., Taga, A., Rust, K., Luciani, E., Marshall, K., Montgomery, E., Mansinghka, A., Singh, R., Zhao, Y., O’Keefe, C., Wang, T.-H., Venkatesan, A., Habela, C. W., & Maragakis, N. J. (2026). Development of a human iPSC-derived corticospinal tract-on-a-chip. Cell reports methods, 6(7), 101457. https://
BibTeX
@article{charalampopoulo
author = {Charalampopoulou, Andriana and Taga, Arens and Rust, Khalil and Luciani, Evelyn and Marshall, Katherine and Montgomery, Elliot and Mansinghka, Anuradha and Singh, Richa and Zhao, Yang and O’Keefe, Christine and Wang, Tza-Huei and Venkatesan, Arun and Habela, Christa Whelan and Maragakis, Nicholas John},
title = {{Development of a human iPSC-derived corticospinal tract-on-a-chip}},
journal = {Cell reports methods},
year = {2026},
month = may,
volume = {6},
number = {7},
pages = {101457},
publisher = {Elsevier},
issn = {2667-2375},
doi = {10.1016/
url = {https://
pmid = {42161268},
pmcid = {PMC13390070}
}
RIS
TY - JOUR
AU - Charalampopoulou, Andriana
AU - Taga, Arens
AU - Rust, Khalil
AU - Luciani, Evelyn
AU - Marshall, Katherine
AU - Montgomery, Elliot
AU - Mansinghka, Anuradha
AU - Singh, Richa
AU - Zhao, Yang
AU - O’Keefe, Christine
AU - Wang, Tza-Huei
AU - Venkatesan, Arun
AU - Habela, Christa Whelan
AU - Maragakis, Nicholas John
TI - Development of a human iPSC-derived corticospinal tract-on-a-chip
T2 - Cell reports methods
J2 - Cell Rep Methods
PY - 2026
DA - 2026/
VL - 6
IS - 7
SP - 101457
SN - 2667-2375
PB - Elsevier
DO - 10.1016/
UR - https://
LA - en
ER -
CSL-JSON
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