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Simple 3D-printed stirred bioreactor enhances retinal organoid production via improved oxygenation.

Overview

Authors: Kyle H Schwab1,2, Philsang Hwang3, Ki Yoon Nam1, Zachary Batz1, Suja Hiriyanna3, Florian Régent1, Nicole Y Morgan4, Peter I Lelkes2, Tiansen Li1,3
ORCID iDs: Tiansen Li
  1. Neurobiology, Neurodegeneration & Repair Laboratory, National Eye Institute, National Institutes of Health, Bethesda, MD, USA
  2. Department of Bioengineering, Temple University, Philadelphia, PA, USA
  3. Ocular Gene Therapy Core, National Eye Institute, National Institutes of Health, Bethesda, MD, USA
  4. Biomedical Engineering and Physical Science Shared Resource, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA
Journal: Cell reports methods, volume 6, issue 8, article 101500
Dates: received 14 August 2025; accepted 18 May 2026; published online 15 June 2026; in print August 2026
Type: Research article · Language: English
License: CC BY-NC-ND
Identifiers: DOI 10.1016/j.crmeth.2026.101500 · PMID 42296971 · PMCID PMC13494550 · OpenAlex W4411487536
Open access: gold, a free copy (OpenAlex)
Status: code on request
Categories: human (organism), cellular / molecular (subfield)
Methods: Spectral & time-frequency, Statistics, Smoothing, state filtering, decompositions, fMRI & imaging
Keywords: human-induced pluripotent stem cells, retinal organoid, 3D-printing, bioreactor, oxygenation, hypoxia, mathematical modeling
MeSH: Bioreactors*, Organoids*, Oxygen*, Printing, Three-Dimensional*, Retina*, Cell Differentiation, Humans, Induced Pluripotent Stem Cells (* major topic)
Topic: Retinal Development and Disorders (Molecular Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: National Eye Institute; National Institutes of Health
Citations: cited by 1 paper (Europe PMC); 46 references in the paper
Research resources: Rabbit polyclonal anti-Rhodopsin RRID:AB_2269503, Rabbit polyclonal anti-ZO-1 RRID:AB_2533456, DAPI RRID:AB_2629482, Sheep polyclonal anti-CHX10 RRID:AB_302279, Rabbit polyclonal anti-Laminin RRID:AB_477163, GraphPad Prism 10 RRID:SCR_002798, ImageJ/FIJI RRID:SCR_003070, Adobe Illustrator RRID:SCR_010279, COMSOL Multiphysics RRID:SCR_014767, Biorender RRID:SCR_018361, Fusion RRID:SCR_025670

Abstract

The abstract is not reproduced here: the paper's license (CC BY-NC-ND) does not allow it. Read it in the paper, at the publisher or on Europe PMC.

Code

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The paper's code and data availability statement is in the Data section.

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Data

Datasets cited

Availability statements

The paper has a data availability statement and a code and data availability statement. Its license (CC BY-NC-ND) does not allow reproducing them here; in short, from what the harvester recognized in them:

  • they point to a dataset: NCBI GEO GSE330121
  • they say that the data are available on request
  • they say that the code is available on request

Read them in the paper: doi.org/10.1016/j.crmeth.2026.101500.

Versions

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Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 9 authors, 7 keywords, 8 MeSH terms, 2 funders, 46 references, 11 RRIDs.

Cite

This paper

Schwab, K. H., Hwang, P., Nam, K. Y., Batz, Z., Hiriyanna, S., Régent, F., Morgan, N. Y., Lelkes, P. I., & Li, T. (2026). Simple 3D-printed stirred bioreactor enhances retinal organoid production via improved oxygenation. Cell reports methods, 6(8), 101500. https://doi.org/10.1016/j.crmeth.2026.101500

BibTeX

@article{schwab2026simple,
author = {Schwab, Kyle H and Hwang, Philsang and Nam, Ki Yoon and Batz, Zachary and Hiriyanna, Suja and Régent, Florian and Morgan, Nicole Y and Lelkes, Peter I and Li, Tiansen},
title = {{Simple 3D-printed stirred bioreactor enhances retinal organoid production via improved oxygenation}},
journal = {Cell reports methods},
year = {2026},
month = jun,
volume = {6},
number = {8},
pages = {101500},
publisher = {Elsevier},
issn = {2667-2375},
doi = {10.1016/j.crmeth.2026.101500},
url = {https://doi.org/10.1016/j.crmeth.2026.101500},
pmid = {42296971},
pmcid = {PMC13494550}
}

RIS

TY - JOUR
AU - Schwab, Kyle H
AU - Hwang, Philsang
AU - Nam, Ki Yoon
AU - Batz, Zachary
AU - Hiriyanna, Suja
AU - Régent, Florian
AU - Morgan, Nicole Y
AU - Lelkes, Peter I
AU - Li, Tiansen
TI - Simple 3D-printed stirred bioreactor enhances retinal organoid production via improved oxygenation
T2 - Cell reports methods
J2 - Cell Rep Methods
PY - 2026
DA - 2026/06/15
VL - 6
IS - 8
SP - 101500
SN - 2667-2375
PB - Elsevier
DO - 10.1016/j.crmeth.2026.101500
UR - https://doi.org/10.1016/j.crmeth.2026.101500
LA - en
ER -

CSL-JSON

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