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Role of dopamine signaling in male courtship suppression induced by confinement stress in <i>Drosophila</i>.

Overview

Authors: Tomohito Sato1, Rana Toyama1, Toshihiro Kitamoto2,3, Takaomi Sakai1
ORCID iDs: Toshihiro Kitamoto, Takaomi Sakai
  1. Department of Biological Sciences, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachioji, Tokyo 192-0397, Japan
  2. Department of Anesthesia and Neuroscience & Pharmacology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
  3. Interdisciplinary Graduate Programs in Genetics and Neuroscience, University of Iowa, Iowa City, IA 52242, USA
Institutions: Tokyo Metropolitan University (Japan); University of Iowa (United States)
Journal: iScience, volume 29, issue 6, article 115906
Dates: received 24 November 2025; accepted 22 April 2026; published online 27 April 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1016/j.isci.2026.115906 · PMID 42181280 · PMCID PMC13196396 · OpenAlex W7155899248
Open access: gold, a free copy (OpenAlex)
Status: code on request
Categories: drosophila (organism), cellular / molecular (subfield)
Methods: Spectral & time-frequency, Statistics, Evoked potentials
Keywords: Biological sciences, Neuroscience, Behavioral neuroscience, Molecular neuroscience
Topic: Neurobiology and Insect Physiology Research (Cellular and Molecular Neuroscience, Neuroscience), according to OpenAlex
Funding: Japan Society for the Promotion of Science (21H02528, 21H00434); Ministry of Education, Culture, Sports, Science and Technology
Citations: not cited yet (Europe PMC); 63 references in the paper
Research resources: RRID:AB_2534072, mouse anti-TH antibody RRID:AB_572268, D. melanogaster: UAS-Dicer2 RRID:BDSC_24650, D. melanogaster: R58E02-GAL4 RRID:BDSC_41347, D. melanogaster: R13F02-GAL4 RRID:BDSC_48571, D. melanogaster: UAS-Dop1R2 RNAi RRID:BDSC_51423, D. melanogaster: nSyb-GAL4 RRID:BDSC_51635, D. melanogaster: UAS-TH RNAi #2 RRID:BDSC_65875, D. melanogaster: MB504B-GAL4 RRID:BDSC_68329, D. melanogaster: UAS-TH RNAi #1 RRID:BDSC_76069, D. melanogaster: Dop1R1KOGal4 RRID:BDSC_84714, D. melanogaster: Dop1R2KOGal4 RRID:BDSC_84715, D. melanogaster: Dop2RKOGal4 RRID:BDSC_84716, D. melanogaster: DopEcRKOGal4 RRID:BDSC_84717, D. melanogaster: TH-GAL4 RRID:BDSC_8848, D. melanogaster: UAS-Dop1R1 RNAi RRID:BDSC_93708, D. melanogaster: UAS-Dop2R RNAi RRID:BDSC_93711, D. melanogaster: NP5945-GAL4 RRID:DGGR_105062, MATLAB (2019a) RRID:SCR_001622, IBM SPSS Statistics (version 26 and 28) RRID:SCR_016479, Excel Statistics (version 4.08) RRID:SCR_017294

Abstract

Stress disturbs physiological and psychological homeostasis across species. In mammals, stress reduces male courtship motivation, but the underlying neuronal mechanisms remain poorly understood. Here, we establish a Drosophila model in which confinement to a small space without complete immobilization—termed small-space (SS) stress—suppresses male courtship behavior. Because stress modulates dopamine signaling in both vertebrates and invertebrates, we examined its role in SS-stress-induced courtship suppression. Pharmacological inhibition and genetic manipulations revealed that dopamine synthesis, release, and reception are required to maintain—but not initiate—the SS-stress-induced suppression of male courtship. Furthermore, dopamine release to and reception within the mushroom body—a brain region involved in higher-order sensory processing—were essential for sustaining courtship inhibition after stress. This SS stress paradigm provides a robust framework for elucidating dopamine-mediated mechanisms that support persistent behavioral changes after stress and contribute to a deeper understanding of the neurobiological basis of stress-related sexual dysfunction.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

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The paper's code and data availability statement is in the Data section.

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Data

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All data reported in this paper will be shared by the lead contact upon request.

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Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

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Version 1, 30 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 4 authors, 4 keywords, 2 funders, 63 references, 21 RRIDs.

Cite

This paper

Sato, T., Toyama, R., Kitamoto, T., & Sakai, T. (2026). Role of dopamine signaling in male courtship suppression induced by confinement stress in <i>Drosophila</i>. iScience, 29(6), 115906. https://doi.org/10.1016/j.isci.2026.115906

BibTeX

@article{sato2026role,
author = {Sato, Tomohito and Toyama, Rana and Kitamoto, Toshihiro and Sakai, Takaomi},
title = {{Role of dopamine signaling in male courtship suppression induced by confinement stress in \<i\>Drosophila\</i\>}},
journal = {iScience},
year = {2026},
month = apr,
volume = {29},
number = {6},
pages = {115906},
publisher = {Elsevier},
issn = {2589-0042},
doi = {10.1016/j.isci.2026.115906},
url = {https://doi.org/10.1016/j.isci.2026.115906},
pmid = {42181280},
pmcid = {PMC13196396}
}

RIS

TY - JOUR
AU - Sato, Tomohito
AU - Toyama, Rana
AU - Kitamoto, Toshihiro
AU - Sakai, Takaomi
TI - Role of dopamine signaling in male courtship suppression induced by confinement stress in <i>Drosophila</i>
T2 - iScience
J2 - iScience
PY - 2026
DA - 2026/04/27
VL - 29
IS - 6
SP - 115906
SN - 2589-0042
PB - Elsevier
DO - 10.1016/j.isci.2026.115906
UR - https://doi.org/10.1016/j.isci.2026.115906
LA - en
ER -

CSL-JSON

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