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Thyroid hormones maintain parvalbumin neuron functions in the mouse neocortex.

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Paper

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The authors' code

Markdown · 1 line · 109 B · no license

  1. Java code for Fiji to identify 8oxo labelling in Tom+ cells from brain slices with Cellpose-based detection

README.md at commit 800843f, no license · at the source

Overview

Authors: Juan Ren1,2, Suzy Markossian1, Romain Guyot1, Denise Aubert1, Fabrice Riet3, Jacques Brocard4, Alice Descamps5, Jiemin Wong2, Dongdong Li6, Bruno Cauli6, Thierry Gallopin5, Frédéric Flamant1, Sabine Richard1
  1. Ecole Normale Supérieure de Lyon, INRAE, CNRS, Institut de Génomique Fonctionnelle de Lyon, 69364 Lyon, France
  2. Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China
  3. CNRS, INSERM, CELPHEDIA, PHENOMIN, Institut Clinique de la Souris (ICS), Université de Strasbourg, 1 rue Laurent Fries, 67404 Illkirch Graffenstaden, France
  4. SFR Biosciences, UAR3444/CNRS, US8/Inserm, ENS de Lyon, UCBL, Lyon, France
  5. Brain Plasticity Unit, CNRS, ESPCI-Paris, PSL Research University, Paris, France
  6. Sorbonne Université, CNRS, INSERM, Neuro-SU, Institut de Biologie Paris-Seine, 7 quai Saint Bernard, 75005 Paris, France
Journal: iScience, volume 29, issue 6, article 115970
Dates: received 9 July 2025; accepted 24 March 2026; published online 26 May 2026
Type: Research article · Language: English
License: CC BY-NC
Identifiers: DOI 10.1016/j.isci.2026.115970 · PMID 42256299 · PMCID PMC13233810 · OpenAlex W4400861055
Open access: gold, a free copy (OpenAlex)
Status: empty repository
Categories: mouse (organism), developmental (subfield)
Methods: Statistics, Machine learning, Evoked potentials, Physiology & signal measures
Keywords: Health sciences
Topic: Genetics and Neurodevelopmental Disorders (Genetics, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: Université Claude Bernard Lyon 1; CNRS (UAR3444); Institut National de la Santé et de la Recherche Médicale (Inserm); ENS de Lyon; ANIRA; PBES; Chinese scholarship Council (# 202106140005); Horizon 2020 (825753); Agence Nationale de la Recherche (French National Research Agency) (ANR-15-CE14-0011-01); Thyromut2 program; Joint Research Institute for Science and Society (JORISS 2022)
Citations: not cited yet (Europe PMC); 62 references in the paper
Research resources: Donkey anti-mouse DyLight 488 RRID:AB_2556746, Donkey anti-mouse DyLight 650 RRID:AB_2556749, Mouse anti-PV, PARV19 RRID:AB_477329, RRID:AB_867461

Abstract

Parvalbumin-expressing GABAergic interneurons are crucial for maintaining excitation/inhibition balance in the neocortex. The postnatal maturation of parvalbumin neurons is highly sensitive to thyroid hormone deficiency. The present study addressed the role of thyroid hormones in mature parvalbumin neurons. Using genetically modified mouse models, we selectively disrupted thyroid hormone signaling in parvalbumin neurons after the onset of parvalbumin expression. Genomic, histological, electrophysiological, sleep, and behavioral analyses were conducted. Mutant mice showed reduced expression of perineuronal net components, decreased neuron excitability, altered gamma oscillations, behavioral hyperactivity, increased wakefulness, delayed sleep onset, and greater susceptibility to seizures. These results demonstrate that thyroid hormones are essential not only for the maturation but also for the maintenance of cortical parvalbumin neuron function after the onset of parvalbumin expression. Disruption of thyroid hormone signaling, during development or adulthood, may thus contribute to neurological and psychiatric disorders involving impaired cortical oscillations.

Reproduced under the paper's license (CC BY-NC), from the paper cited above.

Repository

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jbrocardplatim/Macro_8oxo

License: none: the authors keep all their rights
State: the link answers, verified on 28 September 2026
Evidence: files inventoried
Commit: 800843f92c234eb668e5148b6ca2a735407a5aa2, 28 April 2025
Size: 2 files, 0 scripts
Software Heritage: not archived
Found in: “Data and code availability”
Holds: README
Not found: license file, CITATION.cff, environment file, tests, continuous integration, documentation
Availability: 1 check, the latest on 28 September 2026: the link answers
  • 28 September 2026: the link answers
1 file

The paper's code and data availability statement is in the Data section.

Tracing map

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Data

Data links

Data and code availability

• RNA-seq data are hosted on the GEO database: Gene Expression Omnibus: GSE304025 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi), and the accession number is also provided in the key resources table. • The code for the original macro used to analyze images for the 8-oxo-DG labeling experiment is hosted on the GitHub platform: https://github.com/jbrocardplatim/Macro_8oxo (https://github.com/), and the specific access link is also provided in the key resources table.

Reproduced under the paper's license (CC BY-NC), from the paper cited above.

Versions

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Version 2, 28 September 2026

  • Authors: added Sabine Richard (0000-0003-4639-7034); removed Sabine Richard

Version 1, 28 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 13 authors, 1 keyword, 11 funders, 62 references, 4 RRIDs.

Cite

This paper

Ren, J., Markossian, S., Guyot, R., Aubert, D., Riet, F., Brocard, J., Descamps, A., Wong, J., Li, D., Cauli, B., Gallopin, T., Flamant, F., & Richard, S. (2026). Thyroid hormones maintain parvalbumin neuron functions in the mouse neocortex. iScience, 29(6), 115970. https://doi.org/10.1016/j.isci.2026.115970

BibTeX

@article{ren2026thyroid,
author = {Ren, Juan and Markossian, Suzy and Guyot, Romain and Aubert, Denise and Riet, Fabrice and Brocard, Jacques and Descamps, Alice and Wong, Jiemin and Li, Dongdong and Cauli, Bruno and Gallopin, Thierry and Flamant, Frédéric and Richard, Sabine},
title = {{Thyroid hormones maintain parvalbumin neuron functions in the mouse neocortex}},
journal = {iScience},
year = {2026},
month = may,
volume = {29},
number = {6},
pages = {115970},
publisher = {Elsevier},
issn = {2589-0042},
doi = {10.1016/j.isci.2026.115970},
url = {https://doi.org/10.1016/j.isci.2026.115970},
pmid = {42256299},
pmcid = {PMC13233810}
}

RIS

TY - JOUR
AU - Ren, Juan
AU - Markossian, Suzy
AU - Guyot, Romain
AU - Aubert, Denise
AU - Riet, Fabrice
AU - Brocard, Jacques
AU - Descamps, Alice
AU - Wong, Jiemin
AU - Li, Dongdong
AU - Cauli, Bruno
AU - Gallopin, Thierry
AU - Flamant, Frédéric
AU - Richard, Sabine
TI - Thyroid hormones maintain parvalbumin neuron functions in the mouse neocortex
T2 - iScience
J2 - iScience
PY - 2026
DA - 2026/05/26
VL - 29
IS - 6
SP - 115970
SN - 2589-0042
PB - Elsevier
DO - 10.1016/j.isci.2026.115970
UR - https://doi.org/10.1016/j.isci.2026.115970
LA - en
ER -

CSL-JSON

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