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Ptprz1b phosphatase binds Prickle2 to promote its membrane localization.

Overview

Authors: Yao Le1, Sarka Novotna2, Lorena Agostini Maia2, Nicholas S. Tolwinski3, Christoph Winkler1, Jakub Harnos2
ORCID iDs: Jakub Harnos
  1. Department of Biological Sciences and Centre for Bioimaging Sciences, National University of Singapore, Singapore 117543, Singapore
  2. Department of Experimental Biology, Faculty of Science, Masaryk University, 62500 Brno, Czechia
  3. Programme in Cancer and Stem Cell Biology, Duke-NUS Medical School, Singapore 169857, Singapore
Institutions: National University of Singapore (Singapore); Masaryk University (Czechia); Duke-NUS Medical School (Singapore)
Journal: iScience, volume 29, issue 8, article 116707
Dates: received 13 November 2025; accepted 21 June 2026; published online 13 July 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1016/j.isci.2026.116707 · PMID 42519051 · PMCID PMC13382465 · OpenAlex W7168128784
Open access: gold, a free copy (OpenAlex)
Status: code on request
Categories: zebrafish (organism), cellular / molecular (subfield)
Methods: Statistics, Evoked potentials, Connectivity, fMRI & imaging
Keywords: Planar cell polarity signaling, Vangl/Prickle, protein tyrosine phosphatase receptor, membrane localization
Topic: Cellular transport and secretion (Cell Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: Grant Agency of Masaryk University (MUNI/JS/1952/2025); Czech Ministry of Education, Youth and Sports (CZ.02.01.01/00/22_010/0003229); Singapore Ministry of Education (MOE2016-T3-1-005, MOE-T2EP30221-0008)
Citations: not cited yet (Europe PMC); 77 references in the paper
Research resources: Flag M2 Affinity Gel RRID:AB_10063035, GFP RRID:AB_1286217, Alexa Fluor 568 Anti-Rabbit RRID:AB_143157, Cox IV RRID:AB_2085424, Vangl1 E-3 RRID:AB_2212969, ß-actin RRID:AB_2223172, E-cadherin RRID:AB_2291471, HSP60 RRID:AB_2295614, Cy3 AffiniPure Anti-Rabbit RRID:AB_2307443, Anti-Rabbit IgG Peroxidase antibody RRID:AB_257896, Anti-Mouse IgG Peroxidase antibody RRID:AB_258167, Flag M2 RRID:AB_262044, Alexa Fluor 488 Anti-Mouse RRID:AB_2762823, HA RRID:AB_307019, GFP B-2 RRID:AB_3713277, ß-catenin RRID:AB_397554, myc RRID:AB_439680, Flag RRID:AB_439687, c-myc 9E10 RRID:AB_62726

Abstract

The Wnt/planar cell polarity (PCP) pathway plays a critical role in the development and homeostasis of multicellular organisms. Molecularly, it is organized into two core protein complexes, Vangl/Prickle and Dishevelled/Frizzled. Here, we identify the receptor-type tyrosine phosphatase Ptprz1b as a regulator of Prickle membrane retention. Ptprz1b binds Prickle2 through multiple regions and depends on Vangl through formation of a membrane-competent Prickle2 pool rather than direct Ptprz1b-Vangl binding. Loss of ptprz1b impairs Prickle2 membrane localization in zebrafish embryos and increases its turnover at the plasma membrane, while membrane Vangl2 levels remain unchanged. A catalytic trapping mutant of Ptprz1b shows increased Prickle2 binding but reduced membrane retention, supporting an activity-dependent stabilization mechanism. Ptprz1b deficiency leads to defects in PCP-dependent morphogenetic processes, including impaired convergent extension in zebrafish and neural tube closure defects in Xenopus. Together, these findings identify Ptprz1b as a regulator of membrane-associated Prickle2 required for PCP-dependent morphogenesis in vivo.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper says that its authors' code is available on request: it was not published with the paper, so there is nothing to verify.

The paper's code and data availability statement is in the Data section.

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Data

No dataset and no data link were found in the paper.

Data and code availability

• All data supporting the findings of this study are included in the article and its supplemental information. Original microscopy images, immunoblot data, and quantitative datasets are available from the lead contact upon reasonable request. • This study did not generate custom code. • No large-scale datasets were generated in this study. • Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 6 authors, 4 keywords, 3 funders, 77 references, 19 RRIDs.

Cite

This paper

Le, Y., Novotna, S., Maia, L. A., Tolwinski, N. S., Winkler, C., & Harnos, J. (2026). Ptprz1b phosphatase binds Prickle2 to promote its membrane localization. iScience, 29(8), 116707. https://doi.org/10.1016/j.isci.2026.116707

BibTeX

@article{le2026ptprz1b,
author = {Le, Yao and Novotna, Sarka and Maia, Lorena Agostini and Tolwinski, Nicholas S. and Winkler, Christoph and Harnos, Jakub},
title = {{Ptprz1b phosphatase binds Prickle2 to promote its membrane localization}},
journal = {iScience},
year = {2026},
month = jul,
volume = {29},
number = {8},
pages = {116707},
publisher = {Elsevier},
issn = {2589-0042},
doi = {10.1016/j.isci.2026.116707},
url = {https://doi.org/10.1016/j.isci.2026.116707},
pmid = {42519051},
pmcid = {PMC13382465}
}

RIS

TY - JOUR
AU - Le, Yao
AU - Novotna, Sarka
AU - Maia, Lorena Agostini
AU - Tolwinski, Nicholas S.
AU - Winkler, Christoph
AU - Harnos, Jakub
TI - Ptprz1b phosphatase binds Prickle2 to promote its membrane localization
T2 - iScience
J2 - iScience
PY - 2026
DA - 2026/07/13
VL - 29
IS - 8
SP - 116707
SN - 2589-0042
PB - Elsevier
DO - 10.1016/j.isci.2026.116707
UR - https://doi.org/10.1016/j.isci.2026.116707
LA - en
ER -

CSL-JSON

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