Exemestane increases the number and improves the morphology of cultured pre-antral follicles from transmasculine ovarian cortical tissue.
Overview
- Department of Anatomy and Embryology, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands
- The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, 2333 ZC Leiden, the Netherlands
- Amsterdam UMC Location Vrije University Amsterdam, Department of Obstetrics and Gynecology, 1081 HV Amsterdam, the Netherlands
- Amsterdam UMC, Centre of Expertise on Gender Dysphoria, 1081 HV Amsterdam, the Netherlands
- Amsterdam Reproduction and Development Research Institute, 1081 HV Amsterdam, the Netherlands
- Sequencing Analysis Support Core, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands
- Center for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, the Netherlands
- Department of Gynecology, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands
- Ghent-Fertility and Stem Cell Team (G-FAST), Department of Reproductive Medicine, Ghent University Hospital, 9000 Ghent, Belgium
Abstract
To enhance fertility preservation options, it would be beneficial to develop culture platforms starting from cryopreserved-thawed ovarian cortical tissue. Importantly, ovarian cortical tissue from cisgender females (cOVA) and transmasculine individuals (tOVA) respond differently to culture and may require specific conditions to support optimal folliculogenesis. We explored the role of CYP19A1-inhibitor exemestane on the emergence of pre-antral follicles during culture to equalize the use of cOVA and tOVA. We compared follicular growth, morphology, and distribution between cOVA and tOVA using immunofluorescence and hormonal quantification. The inhibitor reduced the estrogen levels in both OVA types, while enhancing follicular morphology of tOVA. Moreover, treatment with exemestane decreased STAR expression in tOVA regulating progesterone locally. The addition of exemestane during this initial culture period may prove beneficial to obtain pre-antral follicles from tOVA more comparable to cOVA. These findings represent a step toward optimizing culture conditions to support early folliculogenesis.
Reproduced under the paper's license (CC BY), from the paper cited above.
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• Data reported in this paper will be shared by the lead contact upon request. • This paper does not report original code. • Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.
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Versions
The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.
Version 3, 28 September 2026
- Authors: added Hui Cheng (0000-0002-3606-8221); Susana M. Chuva de Sousa Lopes (0000-0003-3866-2803); removed Hui Cheng; Susana M. Chuva de Sousa Lopes
Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 14 authors, 10 keywords, 3 funders, 42 references, 14 RRIDs.
Cite
This paper
Cheng, H., Wei, F., Stolk, T. H., Huirne, J. A., Vugt, W. v., Cats, D., Mei, H., Brewster, K. A., Sánchez-López, E., Giera, M., Pilgram, G. S., van Der Westerlaken, L. A., van Mello, N. M., & Chuva de Sousa Lopes, S. M. (2026). Exemestane increases the number and improves the morphology of cultured pre-antral follicles from transmasculine ovarian cortical tissue. iScience, 29(8), 116992. https://
BibTeX
@article{cheng2026exemes
author = {Cheng, Hui and Wei, Fu and Stolk, Tessa H.R. and Huirne, Judith A. and Vugt, Wouter van and Cats, Davy and Mei, Hailiang and Brewster, Kevin A.J. and Sánchez-López, Elena and Giera, Martin and Pilgram, Gonneke S.K. and van Der Westerlaken, Lucette A.J. and van Mello, Norah M. and Chuva de Sousa Lopes, Susana M.},
title = {{Exemestane increases the number and improves the morphology of cultured pre-antral follicles from transmasculine ovarian cortical tissue}},
journal = {iScience},
year = {2026},
month = aug,
volume = {29},
number = {8},
pages = {116992},
publisher = {Elsevier},
issn = {2589-0042},
doi = {10.1016/
url = {https://
pmid = {42620711},
pmcid = {PMC13486770}
}
RIS
TY - JOUR
AU - Cheng, Hui
AU - Wei, Fu
AU - Stolk, Tessa H.R.
AU - Huirne, Judith A.
AU - Vugt, Wouter van
AU - Cats, Davy
AU - Mei, Hailiang
AU - Brewster, Kevin A.J.
AU - Sánchez-López, Elena
AU - Giera, Martin
AU - Pilgram, Gonneke S.K.
AU - van Der Westerlaken, Lucette A.J.
AU - van Mello, Norah M.
AU - Chuva de Sousa Lopes, Susana M.
TI - Exemestane increases the number and improves the morphology of cultured pre-antral follicles from transmasculine ovarian cortical tissue
T2 - iScience
J2 - iScience
PY - 2026
DA - 2026/
VL - 29
IS - 8
SP - 116992
SN - 2589-0042
PB - Elsevier
DO - 10.1016/
UR - https://
LA - en
ER -
CSL-JSON
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"author": [
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