OSCR

Optimized chemogenetic ablation and regeneration of enteric nervous system neurons in zebrafish.

Overview

Authors: Mujahid Ali Shah1, Katherine K Moran1,2, Helen Rueckert1,3, Abigail V Sharrock4, David F Ackerley4, Jeff S Mumm5, Julia Ganz1
ORCID iDs: Katherine K Moran, David F Ackerley, Julia Ganz
  1. Department of Integrative Biology, Michigan State University, East Lansing, MI, USA
  2. Feinberg School of Medicine, Northwestern University, Chicago, IL, USA
  3. Department of Cell Biology, Developmental and Stem Cell Biology Program, Duke University School of Medicine, Durham, NC, USA
  4. School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand
  5. Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD, USA
Institutions: Michigan State University (United States); Duke University (United States); Victoria University of Wellington (New Zealand); Johns Hopkins University (United States); Johns Hopkins Hospital (United States)
Journal: Stem cell reports, volume 21, issue 7, article 102966
Dates: received 22 December 2025; accepted 19 May 2026; published online 14 July 2026; in print July 2026
Type: Research article · Language: English
License: CC BY-NC-ND
Identifiers: DOI 10.1016/j.stemcr.2026.102966 · PMID 42447850 · PMCID PMC13385441 · OpenAlex W7168294131
Open access: gold, a free copy (OpenAlex)
Status: code on request
Categories: zebrafish (organism), systems (subfield)
Methods: Statistics, Evoked potentials
Keywords: enteric neurons, chemogenetic ablation, functional recovery, gut motility, ENS diseases, immune response
MeSH: Enteric Nervous System*, Nerve Regeneration*, Neurons*, Regeneration*, Zebrafish*, Animals, Animals, Genetically Modified, Chemogenetics (* major topic)
Topic: Congenital gastrointestinal and neural anomalies (Surgery, Medicine), according to OpenAlex
Funding: NIH HHS (R01 OD020376); NIH (R21NS123629); National Science Foundation; National Science Foundation Division of Integrative Organismal Systems (2143267); Marsden Fund (24-VUW-106); American Neurogastroenterology and Motility Society; NINDS NIH HHS (R21 NS123629)
Citations: not cited yet (Europe PMC); 71 references in the paper
Research resources: RRID:AB_11167454, RRID:AB_221448, Mouse anti-GFP monoclonal antibody (3E6) RRID:AB_221568, Rabbit anti-GFP polyclonal antibody RRID:AB_221569, RRID:AB_2886970, Rabbit anti-5-HT (Serotonin) antibody RRID:AB_572263

Abstract

The abstract is not reproduced here: the paper's license (CC BY-NC-ND) does not allow it. Read it in the paper, at the publisher or on Europe PMC.

Code

The paper says that its authors' code is available on request: it was not published with the paper, so there is nothing to verify.

The paper's code and data availability statement is in the Data section.

Tracing map

A tracing map links a paper to the code its authors published: this paper has none (its code is available on request), so it has no map.

Data

No dataset and no data link were found in the paper.

Code and data availability statement

The paper has a code and data availability statement. Its license (CC BY-NC-ND) does not allow reproducing it here; in short, from what the harvester recognized in it:

  • it says that the data are available on request
  • it says that the code is available on request

Read it in the paper: doi.org/10.1016/j.stemcr.2026.102966.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 7 authors, 6 keywords, 8 MeSH terms, 7 funders, 70 references, 6 RRIDs.

Cite

This paper

Shah, M. A., Moran, K. K., Rueckert, H., Sharrock, A. V., Ackerley, D. F., Mumm, J. S., & Ganz, J. (2026). Optimized chemogenetic ablation and regeneration of enteric nervous system neurons in zebrafish. Stem cell reports, 21(7), 102966. https://doi.org/10.1016/j.stemcr.2026.102966

BibTeX

@article{shah2026optimized,
author = {Shah, Mujahid Ali and Moran, Katherine K and Rueckert, Helen and Sharrock, Abigail V and Ackerley, David F and Mumm, Jeff S and Ganz, Julia},
title = {{Optimized chemogenetic ablation and regeneration of enteric nervous system neurons in zebrafish}},
journal = {Stem cell reports},
year = {2026},
month = jul,
volume = {21},
number = {7},
pages = {102966},
publisher = {Elsevier},
issn = {2213-6711},
doi = {10.1016/j.stemcr.2026.102966},
url = {https://doi.org/10.1016/j.stemcr.2026.102966},
pmid = {42447850},
pmcid = {PMC13385441}
}

RIS

TY - JOUR
AU - Shah, Mujahid Ali
AU - Moran, Katherine K
AU - Rueckert, Helen
AU - Sharrock, Abigail V
AU - Ackerley, David F
AU - Mumm, Jeff S
AU - Ganz, Julia
TI - Optimized chemogenetic ablation and regeneration of enteric nervous system neurons in zebrafish
T2 - Stem cell reports
J2 - Stem Cell Reports
PY - 2026
DA - 2026/07/01
VL - 21
IS - 7
SP - 102966
SN - 2213-6711
PB - Elsevier
DO - 10.1016/j.stemcr.2026.102966
UR - https://doi.org/10.1016/j.stemcr.2026.102966
LA - en
ER -

CSL-JSON

{
"id": "10.1016/j.stemcr.2026.102966",
"type": "article-journal",
"title": "Optimized chemogenetic ablation and regeneration of enteric nervous system neurons in zebrafish",
"container-title": "Stem cell reports",
"author": [
{
"family": "Shah",
"given": "Mujahid Ali"
},
{
"family": "Moran",
"given": "Katherine K"
},
{
"family": "Rueckert",
"given": "Helen"
},
{
"family": "Sharrock",
"given": "Abigail V"
},
{
"family": "Ackerley",
"given": "David F"
},
{
"family": "Mumm",
"given": "Jeff S"
},
{
"family": "Ganz",
"given": "Julia"
}
],
"container-title-short": "Stem Cell Reports",
"volume": "21",
"issue": "7",
"page": "102966",
"DOI": "10.1016/j.stemcr.2026.102966",
"PMID": "42447850",
"PMCID": "PMC13385441",
"ISSN": "2213-6711",
"publisher": "Elsevier",
"URL": "https://doi.org/10.1016/j.stemcr.2026.102966",
"language": "en",
"issued": {
"date-parts": [
[
2026,
7,
1
]
]
}
}

Similar papers

The papers with a page that share the most with this one: the tools found in their code, their categories, datasets, cited references and authors, the rarest counting most.

[1] doi:10.1242/dmm.052747 [code]
Cell death analysis of inducible, titratable neurodegenerative disease models in zebrafish and human stem cell-derived retinal organoids.
Journal: Disease models & mechanisms
In common: zebrafish, 5 references, author David F. Ackerley
[2] doi:10.1096/fj.202504890rr
Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish.
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
In common: zebrafish, 4 references
[3] doi:10.1016/j.isci.2026.116206 [code]
Gut distension evokes rapid neural dynamics in vagal and hindbrain populations of larval zebrafish.
Journal: iScience
In common: zebrafish, systems, 2 references
[4] doi:10.1002/glia.70158
A Dynamic Change of Microglial States Occurs During the Transition From Photoreceptor Degeneration to Regeneration in Zebrafish pde6c Mutants.
Journal: Glia
In common: zebrafish, 2 references
[5] doi:10.1021/ehp.6c00394 [code]
Multibehavioral Phenotyping in Early-Life-Stage Zebrafish for Identifying Disruptors of Nonassociative Learning.
Journal: Environmental health perspectives
In common: zebrafish, 2 references
[6] doi:10.1073/pnas.2511442123 [code]
Time-resolved morphological and transcriptomic characterization of early enteric neuron subtype emergence in chick.
Journal: Proceedings of the National Academy of Sciences of the United States of America
In common: 2 references
[7] doi:10.1073/pnas.2614077123 [code]
Resolving cell lineages and gene functions in the developing mouse gastrointestinal tract using in utero transduction.
Journal: Proceedings of the National Academy of Sciences of the United States of America
In common: 2 references
[8] doi:10.1172/jci196113
Western diet induces iron-dependent enteric neurodegeneration via ferroptosis.
Journal: The Journal of clinical investigation
In common: 2 references
[9] doi:10.1126/sciadv.aee5076 [code]
A 3D amphioxus brain atlas illuminates the blueprint of the ancestral chordate brain and highlights unique vertebrate innovations.
Journal: Science advances
In common: 2 references
[10] doi:10.1038/s42003-026-10957-8 [code]
Brain defence by the extracellular matrix protein Cochlin.
Journal: Communications biology
In common: zebrafish, 1 reference

Contribute

The authors of this paper can claim it, correct its record and validate its tracing map, and the maintainers of its code (its owner, or a public member of its organization) correct what it says of their repository; anyone signed in can ask for its removal. Every request goes to OSCR's own machine, which answers it; your account page follows them.

Sign in with ORCID to claim this paper as one of its authors, correct its record or validate its tracing map: when the paper's metadata lists your ORCID iD, you are recognized at once. Maintainers of its code: sign in with GitHub, then claim the repository on your account page.

Request its removal

To ask OSCR to remove this record, the copies of its authors' scripts or its tracing map, use the removal request page: signed in, you say who you are, what to remove and why, then review and confirm the request. Published rules decide every request (how).

Discussion, reproductions, activity

Discussion: questions and error reports about this paper and its code, from signed-in readers and its authors. It opens with sign-in.

Reproductions: reports from readers who ran the authors' code: what they reproduced, with which environment, commit and data. It opens with sign-in.

Activity: what happens around this paper: new versions of its record, its map's validation, discussions and reproductions. It opens with sign-in.