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Protocol for monitoring cytoplasmic Ca<sup>2+</sup> dynamics using Fura Red-AM on a Zeiss Axio Observer microscope.

Overview

Authors: Sabrina Beaumier1, Marc Bartoli1,2
ORCID iDs: Marc Bartoli
  1. Marseille Medical Genetics (MMG), Aix-Marseille Université, INSERM, Bouches-du-Rhône, Marseille 13500, France
  2. CNRS, Marseille, France
Institutions: Inserm (France); Aix-Marseille Université (France); Centre de Génétique Médicale de Marseille (France); Centre National de la Recherche Scientifique (France)
Journal: STAR protocols, volume 7, issue 2, article 104469
Dates: published online 1 April 2026; in print June 2026
Type: Research article · Language: English
License: CC BY-NC-ND
Identifiers: DOI 10.1016/j.xpro.2026.104469 · PMID 41931392 · PMCID PMC13084405 · OpenAlex W7147735147
Open access: gold, a free copy (OpenAlex)
Status: code on request
Categories: histology / microscopy (modality), human (organism), cellular / molecular (subfield)
Methods: Statistics, Evoked potentials, fMRI & imaging, Single-unit activity, calcium imaging
Keywords: Cell culture, Cell-based Assays, Microscopy
MeSH: Calcium*, Cytoplasm*, Animals, HEK293 Cells, Hela Cells, Humans, Microscopy, Fluorescence, ORAI1 Protein (* major topic)
Topic: Advanced Fluorescence Microscopy Techniques (Biophysics, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: AFM
Citations: not cited yet (Europe PMC); 13 references in the paper
Research resources: Hela cells RRID:CVCL_0030, HEK293 cells RRID:CVCL_0045

Abstract

The abstract is not reproduced here: the paper's license (CC BY-NC-ND) does not allow it. Read it in the paper, at the publisher or on Europe PMC.

Code

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Data

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Code and data availability statement

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  • it says that the code is available on request

Read it in the paper: doi.org/10.1016/j.xpro.2026.104469.

Versions

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Version 1, 28 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 2 authors, 3 keywords, 8 MeSH terms, 1 funder, 12 references, 2 RRIDs.

Cite

This paper

Beaumier, S., & Bartoli, M. (2026). Protocol for monitoring cytoplasmic Ca<sup>2+</sup> dynamics using Fura Red-AM on a Zeiss Axio Observer microscope. STAR protocols, 7(2), 104469. https://doi.org/10.1016/j.xpro.2026.104469

BibTeX

@article{beaumier2026protocol,
author = {Beaumier, Sabrina and Bartoli, Marc},
title = {{Protocol for monitoring cytoplasmic Ca\<sup\>2+\</sup\> dynamics using Fura Red-AM on a Zeiss Axio Observer microscope}},
journal = {STAR protocols},
year = {2026},
month = apr,
volume = {7},
number = {2},
pages = {104469},
publisher = {Elsevier},
issn = {2666-1667},
doi = {10.1016/j.xpro.2026.104469},
url = {https://doi.org/10.1016/j.xpro.2026.104469},
pmid = {41931392},
pmcid = {PMC13084405}
}

RIS

TY - JOUR
AU - Beaumier, Sabrina
AU - Bartoli, Marc
TI - Protocol for monitoring cytoplasmic Ca<sup>2+</sup> dynamics using Fura Red-AM on a Zeiss Axio Observer microscope
T2 - STAR protocols
J2 - STAR Protoc
PY - 2026
DA - 2026/04/01
VL - 7
IS - 2
SP - 104469
SN - 2666-1667
PB - Elsevier
DO - 10.1016/j.xpro.2026.104469
UR - https://doi.org/10.1016/j.xpro.2026.104469
LA - en
ER -

CSL-JSON

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"language": "en",
"issued": {
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