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Protocol for Seahorse 3D Mito Stress assay in patient-derived atypical teratoid rhabdoid tumor CHLA-05-ATRT single neurospheres.

Overview

Authors: Stefania Tocci1,2, Jessica W. Tsai1,2,3,4
ORCID iDs: Jessica W. Tsai
  1. Children’s Hospital Los Angeles, Cancer and Blood Disease Institute, Los Angeles, CA, USA
  2. Children’s Hospital Los Angeles, Saban Research Institute, Los Angeles, CA, USA
  3. Keck School of Medicine of University of Southern California, Department of Pediatrics, Los Angeles, CA, USA
  4. USC Norris Comprehensive Cancer Center, Epigenetic Regulation in Cancer Program, Los Angeles, CA, USA
Journal: STAR protocols, volume 7, issue 2, article 104515
Dates: published online 24 April 2026; in print June 2026
Type: Research article · Language: English
License: CC BY-NC-ND
Identifiers: DOI 10.1016/j.xpro.2026.104515 · PMID 42033730 · PMCID PMC13127629 · OpenAlex W7155562136
Open access: gold, a free copy (OpenAlex)
Status: code on request
Categories: human (organism), other condition (population)
Methods: Evoked potentials
Keywords: Cell culture, Cell-based Assays, Metabolism
MeSH: Brain Neoplasms*, Cell Culture Techniques*, Rhabdoid Tumor*, Teratoma*, Cell Line, Tumor, Humans, Spheroids, Cellular, Tumor Cells, Cultured (* major topic)
Topic: Chromatin Remodeling and Cancer (Molecular Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: National Cancer Institute (1K08CA279908-01A1); Rally Foundation; Baldrick’s Foundation; Alex’s Lemonade Stand Foundation; Wright Foundation; Musella Foundation; Albert and Bettie Sacchi Foundation; McKenna Claire Foundation; Elsa U. Pardee Foundation; Margaret E. Early Medical Research Trust; Cure Starts Now Foundation; Brooke Healey Foundation; Melina Michelle Edenfield Foundation; Cure Starts Now Australia; Cure Starts Now Canada; Reflections Of Grace Foundation; Yuvaan Tiwari Foundation; Cure Brain Cancer Foundation; Aubreigh’s Army Foundation; Aidan’s Avengers; DIPG; Love4Lucas Foundation; Anna’s Bake Sale Foundation; Ayla Foundation; Isabella and Marcus Foundation; Love; Chloe Foundation; Lauren’s Fight for Cure; Robert Connor Dawes Foundation; Ryan’s Hope; Gold Hope Project; Abby’s Corner Foundation; DMG; Cancer and Blood Disease Institute; CBDI; Children’s Hospital Los Angeles; CHLA; Saban Research Institute at CHLA; USC Norris Comprehensive Cancer Center
Citations: cited by 1 paper (Europe PMC); 13 references in the paper

Abstract

The abstract is not reproduced here: the paper's license (CC BY-NC-ND) does not allow it. Read it in the paper, at the publisher or on Europe PMC.

Code

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Data

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Code and data availability statement

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  • it says that the code is available on request

Read it in the paper: doi.org/10.1016/j.xpro.2026.104515.

Versions

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Version 1, 30 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 2 authors, 3 keywords, 8 MeSH terms, 38 funders, 13 references.

Cite

This paper

Tocci, S., & Tsai, J. W. (2026). Protocol for Seahorse 3D Mito Stress assay in patient-derived atypical teratoid rhabdoid tumor CHLA-05-ATRT single neurospheres. STAR protocols, 7(2), 104515. https://doi.org/10.1016/j.xpro.2026.104515

BibTeX

@article{tocci2026protocol,
author = {Tocci, Stefania and Tsai, Jessica W.},
title = {{Protocol for Seahorse 3D Mito Stress assay in patient-derived atypical teratoid rhabdoid tumor CHLA-05-ATRT single neurospheres}},
journal = {STAR protocols},
year = {2026},
month = apr,
volume = {7},
number = {2},
pages = {104515},
publisher = {Elsevier},
issn = {2666-1667},
doi = {10.1016/j.xpro.2026.104515},
url = {https://doi.org/10.1016/j.xpro.2026.104515},
pmid = {42033730},
pmcid = {PMC13127629}
}

RIS

TY - JOUR
AU - Tocci, Stefania
AU - Tsai, Jessica W.
TI - Protocol for Seahorse 3D Mito Stress assay in patient-derived atypical teratoid rhabdoid tumor CHLA-05-ATRT single neurospheres
T2 - STAR protocols
J2 - STAR Protoc
PY - 2026
DA - 2026/04/24
VL - 7
IS - 2
SP - 104515
SN - 2666-1667
PB - Elsevier
DO - 10.1016/j.xpro.2026.104515
UR - https://doi.org/10.1016/j.xpro.2026.104515
LA - en
ER -

CSL-JSON

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"container-title": "STAR protocols",
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"container-title-short": "STAR Protoc",
"volume": "7",
"issue": "2",
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"ISSN": "2666-1667",
"publisher": "Elsevier",
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"language": "en",
"issued": {
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}

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