OSCR

Protocol using AGO-APP for isolation and sequencing of argonaute-bound miRNAs in specific neuronal subtypes of the mouse brain.

Overview

Authors: Surbhi Kapoor1, Andrea Erni1, Harold Cremer1, Christophe Beclin1
ORCID iDs: Christophe Beclin
  1. Aix-Marseille Université, Centre National pour la Recherche Scientifique (CNRS), Institut de Biologie du Développement de Marseille (IBDM), Marseille, France
Institutions: Centre National de la Recherche Scientifique (France); Aix-Marseille Université (France); Institut de Biologie du Développement Marseille (France)
Journal: STAR protocols, volume 7, issue 3, article 104720
Dates: published online 4 August 2026; in print August 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1016/j.xpro.2026.104720 · PMID 42550685 · PMCID PMC13464210 · OpenAlex W7172443414
Open access: gold, a free copy (OpenAlex)
Status: data only
Categories: genetics / omics (modality), mouse (organism), cellular / molecular (subfield)
Methods: Statistics, Smoothing, state filtering, decompositions, Evoked potentials
Keywords: Genomics, Sequencing, RNAseq, Model Organisms, Molecular Biology, Gene Expression, Neuroscience
Topic: MicroRNA in disease regulation (Cancer Research, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: Agence Nationale de la Recherche (ANR-21-CE13-0003, ANR-17-CE16-0025, MicroRNAct, Miniature); Fondation pour la Recherche Médicale (Programme \u00E9quipe FRM)
Citations: not cited yet (Europe PMC); 14 references in the paper
Research resources: R version 4.4.2 RRID:SCR_001905, Package ggplot2 of R RRID:SCR_014601, Miniseq RRID:SCR_016378, R Package pheatmap RRID:SCR_016418, R Package DESeq2 RRID:SCR_016533, Bioanalyzer 2100 RRID:SCR_019715, R Package GenomicAlignments RRID:SCR_024236

Abstract

Argonaute protein affinity purification by peptides (Ago-APP) is an immunoprecipitation-based approach that isolates argonaute-bound, putatively active microRNAs (miRNAs) via expression of a T6B peptide. Here, we present a protocol for the application of Ago-APP to olfactory bulb interneurons in mice expressing a Cre-dependent T6B transgene. We outline procedures for maintaining these transgenic mice, performing T6B immunoprecipitation from brain tissue, and preparing miRNA sequencing libraries. This workflow can be applied to specific neuronal subtypes and even subcellular compartments as well as extended to other models, including Drosophila.

For complete details on the use and execution of this protocol, please refer to Kapoor et al.1

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper links to its data, not to its authors' code: see the Data section.

The paper's code and data availability statement is in the Data section.

Tracing map

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Data

Datasets cited

Data and code availability

This manuscript does not generate any new dataset or code.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 3, 28 September 2026

  • Authors: added Christophe Beclin (0000-0003-1923-7238); removed Christophe Beclin

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 4 authors, 7 keywords, 2 funders, 14 references, 7 RRIDs.

Cite

This paper

Kapoor, S., Erni, A., Cremer, H., & Beclin, C. (2026). Protocol using AGO-APP for isolation and sequencing of argonaute-bound miRNAs in specific neuronal subtypes of the mouse brain. STAR protocols, 7(3), 104720. https://doi.org/10.1016/j.xpro.2026.104720

BibTeX

@article{kapoor2026protocol,
author = {Kapoor, Surbhi and Erni, Andrea and Cremer, Harold and Beclin, Christophe},
title = {{Protocol using AGO-APP for isolation and sequencing of argonaute-bound miRNAs in specific neuronal subtypes of the mouse brain}},
journal = {STAR protocols},
year = {2026},
month = aug,
volume = {7},
number = {3},
pages = {104720},
publisher = {Elsevier},
issn = {2666-1667},
doi = {10.1016/j.xpro.2026.104720},
url = {https://doi.org/10.1016/j.xpro.2026.104720},
pmid = {42550685},
pmcid = {PMC13464210}
}

RIS

TY - JOUR
AU - Kapoor, Surbhi
AU - Erni, Andrea
AU - Cremer, Harold
AU - Beclin, Christophe
TI - Protocol using AGO-APP for isolation and sequencing of argonaute-bound miRNAs in specific neuronal subtypes of the mouse brain
T2 - STAR protocols
J2 - STAR Protoc
PY - 2026
DA - 2026/08/04
VL - 7
IS - 3
SP - 104720
SN - 2666-1667
PB - Elsevier
DO - 10.1016/j.xpro.2026.104720
UR - https://doi.org/10.1016/j.xpro.2026.104720
LA - en
ER -

CSL-JSON

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