Protocol using AGO-APP for isolation and sequencing of argonaute-bound miRNAs in specific neuronal subtypes of the mouse brain.
Overview
Abstract
Argonaute protein affinity purification by peptides (Ago-APP) is an immunoprecipitation-base
For complete details on the use and execution of this protocol, please refer to Kapoor et al.1
Reproduced under the paper's license (CC BY), from the paper cited above.
Code
The paper links to its data and to no code of its authors: see the Data section.
The paper's code and data availability statement is in the Data section.
Tracing map
A tracing map links a paper to the code its authors published: this paper has none, so it has no map.
Data
Datasets cited
- arrayexpress:E-MTAB-1512
4 , at ArrayExpress; found in the resources table
Data and code availability
This manuscript does not generate any new dataset or code.
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.
Version 3, 28 September 2026
- Authors: added Christophe Beclin (0000-0003-1923-7238); removed Christophe Beclin
Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 4 authors, 7 keywords, 2 funders, 14 references, 7 RRIDs.
Cite
This paper
Kapoor, S., Erni, A., Cremer, H., & Beclin, C. (2026). Protocol using AGO-APP for isolation and sequencing of argonaute-bound miRNAs in specific neuronal subtypes of the mouse brain. STAR protocols, 7(3), 104720. https://
BibTeX
@article{kapoor2026proto
author = {Kapoor, Surbhi and Erni, Andrea and Cremer, Harold and Beclin, Christophe},
title = {{Protocol using AGO-APP for isolation and sequencing of argonaute-bound miRNAs in specific neuronal subtypes of the mouse brain}},
journal = {STAR protocols},
year = {2026},
month = aug,
volume = {7},
number = {3},
pages = {104720},
publisher = {Elsevier},
issn = {2666-1667},
doi = {10.1016/
url = {https://
pmid = {42550685},
pmcid = {PMC13464210}
}
RIS
TY - JOUR
AU - Kapoor, Surbhi
AU - Erni, Andrea
AU - Cremer, Harold
AU - Beclin, Christophe
TI - Protocol using AGO-APP for isolation and sequencing of argonaute-bound miRNAs in specific neuronal subtypes of the mouse brain
T2 - STAR protocols
J2 - STAR Protoc
PY - 2026
DA - 2026/
VL - 7
IS - 3
SP - 104720
SN - 2666-1667
PB - Elsevier
DO - 10.1016/
UR - https://
LA - en
ER -
CSL-JSON
{
"id": "10.1016/
"type": "article-journal",
"title": "Protocol using AGO-APP for isolation and sequencing of argonaute-bound miRNAs in specific neuronal subtypes of the mouse brain",
"container-title": "STAR protocols",
"author": [
{
"family": "Kapoor",
"given": "Surbhi"
},
{
"family": "Erni",
"given": "Andrea"
},
{
"family": "Cremer",
"given": "Harold"
},
{
"family": "Beclin",
"given": "Christophe"
}
],
"container-title-short":
"volume": "7",
"issue": "3",
"page": "104720",
"DOI": "10.1016/
"PMID": "42550685",
"PMCID": "PMC13464210",
"ISSN": "2666-1667",
"publisher": "Elsevier",
"URL": "https://
"language": "en",
"issued": {
"date-parts": [
[
2026,
8,
4
]
]
}
}
Similar papers
The papers with a page that share the most with this one: the tools found in their code, their categories, datasets, cited references and authors, the rarest counting most.
- [1] doi:10.1016/j.crmeth.2025.101267
- In vivo AGO-APP for cell-type- and compartment-specific miRNA profiling in the mouse brain.Journal: Cell reports methodsIn common: ArrayExpress E-MTAB-15124, genetics / omics, mouse, cellular / molecular, 11 references, author Christophe Beclin
- [2] doi:10.1113/jp288019
- MicroRNA-138-5p suppresses excitatory synaptic strength at the cerebellar input layer.Journal: The Journal of physiologyIn common: mouse, cellular / molecular, 3 references
- [3] doi:10.1371/journal.pgen.1011680
- In vivo AGO-APP identifies a module of microRNAs cooperatively preserving neural progenitors.Journal: PLoS geneticsIn common: 3 references
- [4] doi:10.1016/j.crmeth.2024.100836 [code]
- Genome-wide and cell-type-selective profiling of in vivo small noncoding RNA:target RNA interactions by chimeric RNA sequencing.Journal: Cell reports methodsIn common: genetics / omics, mouse, cellular / molecular, 2 references
- [5] doi:10.1038/s41467-023-44465-8
- Death Induced by Survival gene Elimination (DISE) correlates with neurotoxicity in Alzheimer's disease and aging.Journal: Nature communicationsIn common: mouse, cellular / molecular, 2 references
- [6] doi:10.3389/fnmol.2023.1230942
- Brain cell-specific origin of circulating microRNA biomarkers in experimental temporal lobe epilepsy.Journal: Frontiers in molecular neuroscienceIn common: mouse, 2 references
- [7] doi:10.1016/j.stemcr.2025.102606 [code]
- Computationally resolved neuroprogenitor cell biomarkers associate with human disorders.Journal: Stem cell reportsIn common: genetics / omics, mouse, cellular / molecular, 1 reference
- [8] doi:10.1038/s41586-026-10118-1 [code]
- Single-cell and isoform-specific translational profiling of the mouse brain.Journal: NatureIn common: genetics / omics, mouse, cellular / molecular, 1 reference
- [9] doi:10.1002/jnr.70008 [code]
- An Open-Source Tool for Investigation of Differential RNA Expression Between Spinal Cord Cells of Male and Female Mice.Journal: Journal of neuroscience researchIn common: genetics / omics, mouse, cellular / molecular, 1 reference
- [10] doi:10.1038/s41467-024-47028-7 [code]
- Native-state proteomics of Parvalbumin interneurons identifies unique molecular signatures and vulnerabilities to early Alzheimer's pathology.Journal: Nature communicationsIn common: genetics / omics, mouse, cellular / molecular, 1 reference
Contribute
The authors of this paper can claim it, correct its record and validate its tracing map, and the maintainers of its code (its owner, or a public member of its organization) correct what it says of their repository; anyone signed in can ask for its removal. Every request goes to OSCR's own machine, which answers it; your account page follows them.
Sign in with ORCID to claim this paper as one of its authors, correct its record or validate its tracing map: when the paper's metadata lists your ORCID iD, you are recognized at once. Maintainers of its code: sign in with GitHub, then claim the repository on your account page.
Claim this paper
Correct its record
Say what each link of this record is, remove the ones that are not the paper's, add the ones that are missing. The correction becomes a new version of the record, in its Versions section.
Request its removal
To ask OSCR to remove this record, the copies of its authors' scripts or its tracing map, use the removal request page: signed in, you say who you are, what to remove and why, then review and confirm the request. Published rules decide every request (how).
Discussion, reproductions, activity
Discussion: code errors and mismatches between the code and the paper about this paper and its code, from readers and its authors, beside the paper and its tracing map.
Reproductions: reports from readers who ran the authors' code and did not get the paper's result, what they ran, with which environment, commit and data.
Activity: what happens around this paper: new versions of its record, its map's validation, discussions and reproductions. It opens with sign-in.