OSCR

Mytho/Phaf1 is required to prevent DNA damage and tissue degeneration in Danio rerio.

Overview

Authors: Tommaso Pagliarusco1,2, Anais Franco-Romero1,2, Francesca Terrin3, Filippo Citton1, Ludovica Carducci1, Nicola Facchinello4,5, Camilla Maria Fontana3, Marta Giacomazzo6, Ranieri Verin7, Maria Berica Rasotto3, Lisa Locatello8, Luisa Dalla Valle3, Marco Sandri1,2,9
ORCID iDs: Camilla Maria Fontana, Lisa Locatello, Luisa Dalla Valle, Marco Sandri
  1. Department of Biomedical Sciences, University of Padova, Padova, Italy
  2. Veneto Institute of Molecular Medicine, Padova, Italy
  3. Department of Biology, University of Padova, Padova, Italy
  4. Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy
  5. Neuroscience Institute, Italian Research Council (CNR), Padova, Italy
  6. Department of Comparative Biomedicine and Food Science (BCA), University of Padova, Legnaro, PD Italy
  7. Department of Veterinary Sciences, University of Pisa, Pisa, Italy
  8. Stazione Zoologica Anton Dohrn, Department of Biology and Evolution of Marine Organisms, Fano Marine Center, Fano, Italy
  9. Department of Medicine, McGill University, Montreal, QC Canada
Institutions: University of Padua (Italy); Veneto Institute of Molecular Medicine (Italy); National Research Council (Italy); University of Bologna (Italy); University of Pisa (Italy); Stazione Zoologica Anton Dohrn (Italy); McGill University (Canada)
Journal: Cell death discovery, volume 12, issue 1, article 252
Dates: received 3 November 2025; accepted 26 March 2026; published online 17 April 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1038/s41420-026-03106-x · PMID 41997945 · PMCID PMC13212566 · OpenAlex W7154699556
Open access: gold, a free copy (OpenAlex)
Status: data only
Categories: zebrafish (organism), cellular / molecular (subfield)
Methods: Statistics, Smoothing, state filtering, decompositions, Evoked potentials, Connectivity
Keywords: Macroautophagy, Infertility, Ageing
Topic: FOXO transcription factor regulation (Molecular Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: Fondazione Cassa di Risparmio di Padova e Rovigo
Citations: not cited yet (Europe PMC); 50 references in the paper

Abstract

mytho (Macroautophagy and YouTH Optimizer) is a novel FoxO-dependent gene that has been recently identified to control health- and life-span in Caenorhabditis elegans via autophagy regulation. However, the role of this gene in tissues development and function in vertebrates has not yet been established. To address these issues, we generated a zebrafish mytho KO model and observed that mutants exhibited a higher mortality rate than wild-type (WT) siblings during the first month of life and a lower resistance to oxidative stress. mytho silencing resulted in a decrease in larval locomotor activity and muscle birefringence and caused alteration of adult muscle structure. Autophagy impairment was confirmed in tissues with the highest mytho expression such as brain, muscle and testis. Finally, mutants showed tissue degeneration in pancreas, retina and muscle, morphological alterations in gonads of both sexes and a reduction of reproductive capabilities of males. Importantly, males presented a higher incidence of seminomas, a testicular cancer. The increased susceptibility to cancer is associated with an enhanced DNA fragmentation in sperm cells. In conclusion, this study highlights the key role of Mytho in maintaining proper tissue function and DNA integrity.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper links to its data, not to its authors' code: see the Data section.

Tracing map

A tracing map links a paper to the code its authors published: this paper has none, so it has no map.

Data

Datasets cited

Data availability

The datasets generated and analyzed during the current study are available in NCBI’s Gene Expression Omnibus (Edgar et al., 2002) and are accessible through GEO Series accession number GSE317380 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE317380) (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE317380).

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 29 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 13 authors, 3 keywords, 1 funder, 46 references.

Cite

This paper

Pagliarusco, T., Franco-Romero, A., Terrin, F., Citton, F., Carducci, L., Facchinello, N., Fontana, C. M., Giacomazzo, M., Verin, R., Rasotto, M. B., Locatello, L., Dalla Valle, L., & Sandri, M. (2026). Mytho/Phaf1 is required to prevent DNA damage and tissue degeneration in Danio rerio. Cell death discovery, 12(1), 252. https://doi.org/10.1038/s41420-026-03106-x

BibTeX

@article{pagliarusco2026mytho,
author = {Pagliarusco, Tommaso and Franco-Romero, Anais and Terrin, Francesca and Citton, Filippo and Carducci, Ludovica and Facchinello, Nicola and Fontana, Camilla Maria and Giacomazzo, Marta and Verin, Ranieri and Rasotto, Maria Berica and Locatello, Lisa and Dalla Valle, Luisa and Sandri, Marco},
title = {{Mytho/Phaf1 is required to prevent DNA damage and tissue degeneration in Danio rerio}},
journal = {Cell death discovery},
year = {2026},
month = apr,
volume = {12},
number = {1},
pages = {252},
publisher = {Nature Publishing Group},
issn = {2058-7716},
doi = {10.1038/s41420-026-03106-x},
url = {https://doi.org/10.1038/s41420-026-03106-x},
pmid = {41997945},
pmcid = {PMC13212566}
}

RIS

TY - JOUR
AU - Pagliarusco, Tommaso
AU - Franco-Romero, Anais
AU - Terrin, Francesca
AU - Citton, Filippo
AU - Carducci, Ludovica
AU - Facchinello, Nicola
AU - Fontana, Camilla Maria
AU - Giacomazzo, Marta
AU - Verin, Ranieri
AU - Rasotto, Maria Berica
AU - Locatello, Lisa
AU - Dalla Valle, Luisa
AU - Sandri, Marco
TI - Mytho/Phaf1 is required to prevent DNA damage and tissue degeneration in Danio rerio
T2 - Cell death discovery
J2 - Cell Death Discov
PY - 2026
DA - 2026/04/17
VL - 12
IS - 1
SP - 252
SN - 2058-7716
PB - Nature Publishing Group
DO - 10.1038/s41420-026-03106-x
UR - https://doi.org/10.1038/s41420-026-03106-x
LA - en
ER -

CSL-JSON

{
"id": "10.1038/s41420-026-03106-x",
"type": "article-journal",
"title": "Mytho/Phaf1 is required to prevent DNA damage and tissue degeneration in Danio rerio",
"container-title": "Cell death discovery",
"author": [
{
"family": "Pagliarusco",
"given": "Tommaso"
},
{
"family": "Franco-Romero",
"given": "Anais"
},
{
"family": "Terrin",
"given": "Francesca"
},
{
"family": "Citton",
"given": "Filippo"
},
{
"family": "Carducci",
"given": "Ludovica"
},
{
"family": "Facchinello",
"given": "Nicola"
},
{
"family": "Fontana",
"given": "Camilla Maria"
},
{
"family": "Giacomazzo",
"given": "Marta"
},
{
"family": "Verin",
"given": "Ranieri"
},
{
"family": "Rasotto",
"given": "Maria Berica"
},
{
"family": "Locatello",
"given": "Lisa"
},
{
"family": "Dalla Valle",
"given": "Luisa"
},
{
"family": "Sandri",
"given": "Marco"
}
],
"container-title-short": "Cell Death Discov",
"volume": "12",
"issue": "1",
"page": "252",
"DOI": "10.1038/s41420-026-03106-x",
"PMID": "41997945",
"PMCID": "PMC13212566",
"ISSN": "2058-7716",
"publisher": "Nature Publishing Group",
"URL": "https://doi.org/10.1038/s41420-026-03106-x",
"language": "en",
"issued": {
"date-parts": [
[
2026,
4,
17
]
]
}
}

Similar papers

The papers with a page that share the most with this one: the tools found in their code, their categories, datasets, cited references and authors, the rarest counting most.

[1] doi:10.1186/s12929-026-01249-8
β-Sitosterol β-D-glucoside (BSSG) triggers intestinal inflammation in zebrafish and mouse models prior to neurodegeneration onset.
Journal: Journal of biomedical science
In common: zebrafish, cellular / molecular, 1 reference, author Luisa Dalla Valle
[2] doi:10.7554/elife.109104 [code]
Complementary vertebrate <i>Wac</i> models exhibit phenotypes relevant to DeSanto-Shinawi Syndrome.
Journal: eLife
In common: zebrafish, cellular / molecular, 3 references
[3] doi:10.1186/s13041-026-01300-7
Hippocampal transcriptome profiling in a 22q11.2 deletion syndrome mouse model: comparison with human schizophrenia.
Journal: Molecular brain
In common: cellular / molecular, 3 references
[4] doi:10.1038/s41467-026-70989-w [code]
MIC-Drop-seq: scalable single-cell phenotyping of mutant vertebrate embryos.
Journal: Nature communications
In common: zebrafish, 2 references
[5] doi:10.1016/j.xgen.2026.101278 [code]
Single-cell profiling of DNA methylation in autism spectrum disorder prefrontal cortex reveals distinct regulatory and aging signatures.
Journal: Cell genomics
In common: 4 references
[6] doi:10.1126/sciadv.adt2527
NPAS3-regulated astrocyte mitochondrial bioenergetics is required for cognition.
Journal: Science advances
In common: cellular / molecular, 3 references
[7] doi:10.1096/fj.202504243r
Maternal Choline Supplementation in a Mouse Model of Down Syndrome and Alzheimer's Disease Generates Unique Expression Profile Mosaics Within Three Hippocampal Excitatory Neuronal Populations.
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
In common: 3 references
[8] doi:10.1038/s41467-026-74753-y [code]
A human-specific microRNA controls the timing of excitatory synaptogenesis.
Journal: Nature communications
In common: cellular / molecular, 3 references
[9] doi:10.1038/s41467-026-74153-2 [code]
Regional, functional and transcriptomic decoding of multidimensional brain structure alterations in obsessive-compulsive disorder.
Journal: Nature communications
In common: cellular / molecular, 3 references
[10] doi:10.1038/s41586-026-10679-1 [code]
Cortical development dynamics across autism spectrum disorder mouse models.
Journal: Nature
In common: cellular / molecular, 3 references

Contribute

The authors of this paper can claim it, correct its record and validate its tracing map, and the maintainers of its code (its owner, or a public member of its organization) correct what it says of their repository; anyone signed in can ask for its removal. Every request goes to OSCR's own machine, which answers it; your account page follows them.

Sign in with ORCID to claim this paper as one of its authors, correct its record or validate its tracing map: when the paper's metadata lists your ORCID iD, you are recognized at once. Maintainers of its code: sign in with GitHub, then claim the repository on your account page.

Request its removal

To ask OSCR to remove this record, the copies of its authors' scripts or its tracing map, use the removal request page: signed in, you say who you are, what to remove and why, then review and confirm the request. Published rules decide every request (how).

Discussion, reproductions, activity

Discussion: questions and error reports about this paper and its code, from signed-in readers and its authors. It opens with sign-in.

Reproductions: reports from readers who ran the authors' code: what they reproduced, with which environment, commit and data. It opens with sign-in.

Activity: what happens around this paper: new versions of its record, its map's validation, discussions and reproductions. It opens with sign-in.