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Moderate electrical muscle stimulation during voluntary movement does not disrupt the sense of agency or ownership.

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  1. # Project Title: Moderate Electrical Muscle Stimulation During Voluntary Movement Does Not Disrupt the Sense of Agency or Ownership
  2. We investigated the sense of agency, sense of ownership, and electroencephalogram (EEG) in situations where participants moved their wrists voluntarily, or where electrical muscle stimulation (EMS) was applied either during voluntary movement or at rest. The EMS intensity was varied from the motor threshold to the maximum level at which participants did not experience pain or discomfort.
  3. <!-- TABLE OF CONTENTS -->
  4. ## Table of Contents
  5. - [Project Title](#project-title)
  6. - [Table of Contents](#table-of-contents)
  7. - [About the Project](#about-the-project)
  8. - [License](#license)
  9. - [Attribution and academic use](#attribution-and-academic-use)
  10. - [File structure and overview](#file-structure-and-overview)
  11. <!-- ABOUT THE PROJECT -->
  12. ## About the Project
  13. **Date**: November, 2025
  14. **Researcher(s)**:
  15. - Fumina Mori ([email hidden])
  16. - Dai Yanagihara ([email hidden])
  17. ### License
  18. The code in this project is released under CC BY-ND.
  19. ### Attribution and academic use
  20. For academic use, use presistent data from DOI. This is a persistent copy of the data. Version number refer to the date. Please cite:
  21. Mori, F. (2025). Moderate Electrical Muscle Stimulation During Voluntary Movement Does Not Disrupt the Sense of Agency or Ownership (Version v2025.11.4) [Data set]. Zenodo. (https://doi.org/10.5281/zenodo.17519975)
  22. <!-- FILE STRUCTURE AND OVERVIEW -->
  23. # File structure and overview
  24. ## File tree
  25. ├── 01/ to 18/ (Common structure)
  26. │ └── 001/
  27. │       ├── con1_timer.mat
  28. │       ├── con21_timer.mat
  29. │       ├── myTimer_task.mat
  30. │   ├── 002/, 003/, 004/
  31. │       ├── con1_timer.mat
  32. │       ├── con22_timer.mat
  33. │       ├── con31_timer.mat
  34. │       └── myTimer_task.mat
  35. │   ├── GonioData.mat
  36. │   ├── PS_dB.mat
  37. │   ├── run_001.mat ... run_004.mat
  38. ├── myScores.mat
  39. ├── participants_list.csv
  40. └── program/
  41. ├── 1_preprocess
  42. ├── 2_survey
  43. ├── 3_EEG
  44. ├── 4_movement
  45. ├── dataset
  46. └── use_ID_ch.mat
  47. ## overview
  48. ### file 01 to 18
  49. Each file contains data for each participant.
  50. #### file 001 to 004
  51. Each file contains the timing of the cue in each run.
  52. - con1.mat: timing of the resting condition
  53. - con21.mat: timing of the V condition, where participants moved their wrist voluntarily. EMS was not applied.
  54. - con22.mat: timing of the V+WS/V+MS/V+SS condition, where participants moved their wrist voluntarily, and EMS was applied at the same time.
  55. - con31.mat: timing of the WS/MS/SS condition, where EMS was applied during resting.
  56. All .mat files are created by classify\_trigger.m. For details, please refer to the relevant section.
  57. Abbreviations: V, voluntary movement; WS, weak stimulation; MS, medium stimulation; SS, strong stimulation; EMS, electrical muscle stimulation.
  58. #### GonioData.mat
  59. This file contains the value of the goniometer.
  60. - $degTarget$ is the maximum angle of the palmar flexion. The calculation method is explained later.
  61. - $F$ is the 10-s data of the goniometer and a two-row matrix.
  62. - $nFrq$ is the sampling rate.
  63. How to calculate $degTarget$
  64. - The data in the first row reflects wrist abduction and adduction, while the data in the second row reflects wrist flexion and extension. The data in the second row is used in the research. The row value of the goniometer was transformed into the angle by the following formula:
  65. $a = (1.18-0.01)/90$,
  66. $b = 0.01$,
  67. $degTarget = -(\bar{y}-b)/a$,
  68. where $a$ and $b$ are the goniometer-specific values written on the goniometer's package, and $\bar{y}$ is the mean value in the second-row of F.
  69. #### PS\_dB.mat
  70. This file contains the information of EEG power created by calculate\_PSD.m. For details, please refer to the relevant section.
  71. #### run001.mat ... run004.mat
  72. This file contains the value of the EEG.
  73. - $F$ is a matrix. The first to 11th rows are EEG data. Each row corresponds to an EEG channel (F3, Fz, F4, T3, C3, Cz, C4, T4, P3, Pz and P4). The 12th row is electro-oculography data. The 13th corresponds to wrist abduction and adduction. The 14th corresponds to wrist flexion and extension.
  74. - $myTimer\_cue$ contains the information of the timing of task and trial start. To determine which task or trial's start time that timing corresponds to, refer to the same row in $typeCue$.
  75. - $myTimer\_move$ contains the information of the timing when the participant moved their wrist voluntarily in the V+WS/V+MS/V+SS condition.
  76. - $nFrq$ is the sampling rate.
  77. - $typeCue$ contains the information of task or trial type.
  78. - 1: the start time of the resting condition
  79. - 2: the start time of the V or V+WS/V+MS/V+SS condition.
  80. - 21: the start time of each trial in the V condition.
  81. - 22: the start time of each trial in the V+WS/V+MS/V+SS condition.
  82. - 3: the start time of the WS/MS/SS condition.
  83. - 31: the start time of each trial in the WS/MS/SS condition.
  84. ### program
  85. #### 1_preprocess
  86. In analysis, we used the program in the following order:
  87. filter_EEG.m
  88. classify_trigger.m
  89. calculate_PSD.m
  90. select_ID.m
  91. make_dataset_PSD.m
  92. make_dataset_survayScore.m
  93. check_omitEpochNumber.m and check_omitEpochNumber_betweenConditioin.m is additional program to check the number of omitted epochs in detail.
  94. ##### calculate_PSD.m
  95. This program calculates the EEG power spectral density in dB units for each participant and saves it to PS_dB.mat.
  96. Note:
  97. This program utilizes trial start timing information; therefore, execute classify_trigger.m first. If you wish to analyze filtered data, execute filter_EEG.m beforehand.
  98. --Saved Variables--
  99. [eeg_PS_TimeFreqTrialMean]
  100. Each field contains the time-, frequency-band-, trial-averaged power spectral density for each condition. "con*" means the condition (con1: resting, con21: voluntary movement without EMS (V condition), con22: voluntary movement with EMS (V+WS/MS/SS condition), con31: EMS only (WS/MS/SS condition)). The matrices within each field correspond to EEG electrodes (rows 1-11) and the respective alpha (column 1) and beta (column 2) band.
  101. [eeg\_PS\_relative]
  102. The difference between the time-, frequency-band-, trial-averaged power spectral density for the seven conditions (V, V+WS/MS/SS, WS/MS/SS) and that for the resting state. The meaning of the field names and the structure of the matrices in each field are the same as for eeg_PS_TimeFreqTrialMean.
  103. ##### check_omitEpochNumber.m
  104. This program counts the number of omitted epoch. To count the epoch with body movement observed in the video, set omit_noise = 1. To count the epoch where EMS was not presented during voluntary movement, set omit_epoch_withoutEMS = 0. The number of omitted epoch is summarized in table "T".
  105. ##### check_omitEpochNumber_betweenConditioin.m
  106. This program compare the difference of tnumber of omitted epoch between conditions. Use this program after classify_trigger.m. Table variable "results_Friedman" stores the results of the Friedman test. Table variable "results_multcompare" stores the results of the multiple comparisons.
  107. ##### classify_trigger.m
  108. This program creates con\*\_timer.mat and myTimer\_task.mat.
  109. [con\*\_timer.mat]
  110. "con\*" means the condition (con1: resting, con21: voluntary movement without EMS (V condition), con22: voluntary movement with EMS (V+WS/MS/SS condition), con31: EMS only (WS/MS/SS condition)). This file contains the information of the start time for analysis per trial in each condition. The file is used when manually importing the trigger timing (start time for analysis per trial) after importing data into Brainstorm.
  111. [myTimer_task.mat]
  112. This file has the fields named "con\*". Each field contains the information of the start time for analysis per trial in each condition. The file is used in following analysis.
  113. [removal of the period with body movement]
  114. For each participant and each run, after confirming the period containing body movement, the information for that period was described as follows.
  115. t_noise.run\* = [ts_1, te_1; ...
  116. ts_2, te_2; ...
  117. ts_3, te_3];
  118. ts_n and te_n are the start and end times of the period containing the n-th body movement.
  119. If you want to omit the noisy period from the analysis, set the omit\_noise variable to 1.
  120. The presence of body movements was confirmed by reviewing the EEG data and video. The video is not publicly available due to concerns regarding the privacy of the participants.
  121. ##### filter_EEG.m
  122. This program applies a bandpass filter to the raw data from all participants across all runs in a single batch. It uses the bandpass function included in Brainstorm (https://neuroimage.usc.edu/brainstorm/Introduction), which must be installed beforehand.
  123. ##### make_dataset_PSD.m
  124. This program creates a dataset for analysis in .txt format, organized by frequency and channel. The text file contains a matrix where rows correspond to the selected participants and columns correspond to the task conditions (1: V, 2: V+WS, 3: WS, 4: V+MS, 5: MS, 6: V+SS, 7: SS).
  125. Note: This program utilizes the information of participants and channels to be evaluated, therefore execute select_ID_and_channel.m beforehand.
  126. ##### make_dataset_survayScore.m
  127. This program creates a dataset for analysis in .txt format, organized by the survey constructs. The text file contains a matrix where rows correspond to all participants and columns correspond to the task conditions (1: V, 2: V+WS, 3: WS, 4: V+MS, 5: MS, 6: V+SS, 7: SS).
  128. ##### select_ID_and_channel.m
  129. This program selects the participants and channels to be evaluated in the subsequent analysis. First, it excludes participants from each channel and frequency band whose power during voluntary movement exceeds that of their resting condition. Next, it omits participants who exhibited an outlier in one or more of the seven conditions. Finally, a Wilcoxon signed-rank test is performed to select channels where power decreased during voluntary movement compared to resting conditions.
  130. Note: This program utilizes the PSD data; therefore, execute calculate_PSD.m beforehand.
  131. The information about participants to be evaluated for each channel and for each band is saved as 'use_ID_ch.mat'. The .mat file contains 'ID_use'. 'ID_use' contains the frequency band field. This is an 18 × 11 matrix, where the row corresponds to the participant ID and the column to the EEG channel (F3, Fz, F4, T3, C3, Cz, C4, T4, P3, Pz, P4).'1' indicates that the corresponding participant be used in the subsequent analysis, while '0' indicates the opposite.
  132. #### 2_survey
  133. In analysis, we used the program in the following order:
  134. check_negativeCorrelation_ofScore.m
  135. check_differenceBetweenConditions
  136. check_correlation_surveyItems.m
  137. check_interaction_SoAandSoO.m
  138. scores_forInverseCorrelation.mat will be load in check_negativeCorrelation_ofScore.m.
  139. ##### check_correlation_surveyItems.m
  140. This program examines the correlations among the four items in the questionnaire. It calculates the correlation coefficient using a Linear Mixed Model. The statistical results are stored in table variable ResultSummary.
  141. ##### check_differenceBetweenConditions
  142. This program compare the difference of score between conditions. Use this program after make_dataset_surveyScore.m. Table variable "results_Friedman" stores the results of the Friedman test. Table variable "results_multcompare" stores the results of the multiple comparisons.
  143. ##### check_interaction_SoAandSoO.m
  144. This program performs the linear mixed-effects analysis to examine the differences in the decline of SoA and SoO scores. The statistical results are stored in table variable "statsTable".
  145. ##### check_negativeCorrelation_ofScore.m
  146. This program confirms that Q1 and Q2, and Q3 and Q4, exhibit negative correlation.
  147. It reads scores for each question from scores_forInverseCorrelation.mat, with 18 (ID) × 7 (condition) rows. It calculates the correlation coefficient using a Linear Mixed Model.
  148. The statistical results are stored in table variable ResultSummary.
  149. ##### scores_forInverseCorrelation.mat
  150. This file is load in check_negativeCorrelation_ofScore.m. The file contains variable named Condition, ID, q1, q2, q3, and q4. q1, q2, q3, and q4 corresponds to the score of Q1 to Q4 in the survey. Each variable contains 126(18(ID) × 7 (condition)) scores. To determine which ID and condition each row's value corresponds to, refer to the same row in the ID and Condition variables.
  151. #### 3_EEG
  152. In analysis, we used the program in the following order:
  153. correct_FDR_for_wilcoxon.m
  154. check_differenceFromResting.m
  155. correct_FDR_for_friedman.m
  156. check_PSD_betweenConditions.m
  157. ##### correct_FDR_for_wilcoxon.m
  158. This program performs a Wilcoxon signed-rank test and apply FDR to examine the differences in PSD between the resting state and each condition. It calculates FDR corrected q. To calculate z-score, effect size (r), and 95%CI, run "check_differenceFromResting.m". Q_matrix is a n_ch x n_condition matrix and contains corrected q.
  159. ##### check_differenceFromResting.m
  160. This program performs a Wilcoxon signed-rank test to examine the differences in PSD between the resting state and each condition. Table variable "resultsTable" stores the results of the test.
  161. ##### correct_FDR_for_friedman.m
  162. This program performs a Friedman test and apply FDR correction. It calculates FDR corrected q value (Q). To obtain chi2 value and Kendall's W, and to perform post hoc analysis, run "check_PSD_betweenConditions.m"
  163. ##### check_PSD_betweenConditions
  164. This program compare the difference of PSD between conditions. Use this program after make_dataset_PSD.m. Table variable "results_Friedman" stores the results of the Friedman test. Table variable "results_multcompare" stores the results of the multiple comparisons.
  165. #### 4_movement
  166. In analysis, we used the program in the following order:
  167. check_gonioData.m
  168. check_correlation_AngleCurrent.m
  169. compare_maxAngle.m
  170. check_cue2EMS_lag.m
  171. ##### check_correlation_AngleCurrent.m
  172. This program examines the correlation between the intensity of EMS and the angle of the wrist during movement induced by EMS using Linear Mixed Model.
  173. ##### check_cue2EMS_lag.m
  174. This prgoram calculate the average and standard deviation of the time lag between the cue and EMS presentation in V+WS, V+MS and V+SS condition in each participant. The results are stored in 18 (ID) × 3 (condition) matrix in result_lag.txt. Each row corresponds to a participant. The first column is the V+WS condition, the second column is the V+MS condition, and the third column is the V+SS condition. Each element represents the average value (standard deviation) of the time lag between the cue and EMS presentation.
  175. ##### check_gonioData.m
  176. This program performs averaging of the wrist angle from 100 ms before the cue to 2000 ms after the cue for each condition. The baseline period is the 100 ms before the cue.
  177. The averaged waveform (gonio_all) and maximum angle (gonio_max) are saved in gonio_data.mat. gonio_all is a 7(condition)×2101(number of samples)×18(ID) matrix. gonio_max is a 7(condition)×18(ID) matrix.
  178. Figure 1 shows the wrist angle changes under the V condition. Figure 2 shows the wrist angle changes under the V+WS, V+MS, and V+SS conditions. Figure 3 shows the wrist angle changes under the WS, MS, and SS conditions.
  179. ##### compare_maxAngle.m
  180. This program performs a Wilcoxon signed-rank test to examine the differences in PSD between V vs. V+WS, V vs. V+MS or V vs. V+ SS condition. Use this program after check_gonioData.m Table variable "resultsTable" stores the results of the test.
  181. ##### Current_Angle_List.mat
  182. Variable tbl contains the current values for weak, medium, and strong EMS, along with the maximum angle of wrist movement induced by each EMS level during rest. The data are listed in order of participants' ID and EMS intensity (weakest to strongest).
  183. ##### gonio_data.mat
  184. output of check_gonioData.m. The averaged waveform (gonio_all) and maximum angle (gonio_max) are saved in gonio_data.mat. gonio_all is a 7(condition)×2101(number of samples)×18(ID) matrix. gonio_max is a 7(condition)×18(ID) matrix.
  185. ##### result_lag.txt
  186. output of check_cue2EMS_lag. Each row corresponds to a participant. The first column is the V+WS condition, the second column is the V+MS condition, and the third column is the V+SS condition. Each element represents the average value (standard deviation) of the time lag between the cue and EMS presentation.
  187. #### dataset
  188. Out put of 'make_SPSSdataset_PSD.m' and 'make_SPSSdataset_survayScore.m.' are stored.
  189. #### use_ID.mat
  190. Out put of 'select_ID.m' in 1_preprocess
  191. ### participants_list.csv
  192. This file contains the information of the participants' ID, age, sex and timing of the noisy period with body movement.
  193. To detect the noisy period, we used the video, however, the video is not public for the protection of personal information.
  194. ### myScores.mat
  195. This file has a variable with the same name ($myScores$).
  196. $myScores$ has four fields named "SoA", "SoO", "Fatigue" and "Emotion."
  197. Each field has a matrix. Rows correspond to all participants, and columns correspond to the task conditions (1: V, 2: V+WS, 3: WS, 4: V+MS, 5: MS, 6: V+SS, 7: SS).
  198. Abbreviations: SoA, sense of agency; SoO, sense of ownership, V, voluntary movement; WS, weak stimulation; MS, medium stimulation; SS, strong stimulation.

README.md, under CC-BY-4.0 · at the source

Overview

Authors: Fumina Mori1, Dai Yanagihara1,2
  1. Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Building 9, 3-8-1 Komaba, Meguro-ku, Tokyo, 153-8902 Japan
  2. RIKEN Center for Brain Science, 2-1 Hirosawa, Wako City, Saitama 351-0198 Japan
Journal: Scientific reports, volume 16, issue 1, article 27488
Dates: received 6 November 2025; accepted 9 June 2026; published online 16 June 2026
Type: Research article · Language: English
License: CC BY-NC-ND
Identifiers: DOI 10.1038/s41598-026-57714-9 · PMID 42304073 · PMCID PMC13534502 · OpenAlex W4417233355
Open access: gold, a free copy (OpenAlex)
Status: code verified
Categories: EEG (modality), human (organism), developmental (subfield)
Methods: Spectral & time-frequency, Statistics, Smoothing, state filtering, decompositions, Preprocessing, Physiology & signal measures
Keywords: Electrical muscle stimulation, Sense of agency, Sense of ownership, Electroencephalography, Wrist, Muscles, Neuroscience, Physiology
MeSH: Electric Stimulation*, Muscle, Skeletal*, Sense of Agency*, Adolescent, Adult, Brain, Electroencephalography, Female, Humans, Male, Movement, Young Adult (* major topic)
Topic: Free Will and Agency (Cognitive Neuroscience, Neuroscience), according to OpenAlex
Funding: New Energy and Industrial Technology Development Organization (JPJ012495)
Citations: not cited yet (Europe PMC); 42 references in the paper

Abstract

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Zenodo 18678186

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Found in: “Data availability”
Holds: README
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This paper

Mori, F., & Yanagihara, D. (2026). Moderate electrical muscle stimulation during voluntary movement does not disrupt the sense of agency or ownership. Scientific reports, 16(1), 27488. https://doi.org/10.1038/s41598-026-57714-9

BibTeX

@article{mori2026moderate,
author = {Mori, Fumina and Yanagihara, Dai},
title = {{Moderate electrical muscle stimulation during voluntary movement does not disrupt the sense of agency or ownership}},
journal = {Scientific reports},
year = {2026},
month = jun,
volume = {16},
number = {1},
pages = {27488},
publisher = {Nature Publishing Group},
issn = {2045-2322},
doi = {10.1038/s41598-026-57714-9},
url = {https://doi.org/10.1038/s41598-026-57714-9},
pmid = {42304073},
pmcid = {PMC13534502}
}

RIS

TY - JOUR
AU - Mori, Fumina
AU - Yanagihara, Dai
TI - Moderate electrical muscle stimulation during voluntary movement does not disrupt the sense of agency or ownership
T2 - Scientific reports
J2 - Sci Rep
PY - 2026
DA - 2026/06/16
VL - 16
IS - 1
SP - 27488
SN - 2045-2322
PB - Nature Publishing Group
DO - 10.1038/s41598-026-57714-9
UR - https://doi.org/10.1038/s41598-026-57714-9
LA - en
ER -

CSL-JSON

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