Mechanical constraints regulate embryonic stem cell mitosis in the developing brain.
Paper
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The authors' code
Markdown · 9 lines · 722 B · MIT
- # MitoticCellVolume_EB1cometAnalysis
- Those codes were tested in Fiji 1.54p on Windows and Mac.
- These codes were developed as part of the following article: *Brain biomechanics governs mitotic fidelity of embryonic neural progenitors* by V. Marthiens et al., available here: https://www.biorxiv.org/content/10.1101/2025.07.31.667957v1
- ## Licence
- This project is subject to a specific license from the Institut Curie. Please refer to the file Logiciel MitoticCellVolume_EB1cometAnalysis.licence for more details.
- *******************************************
- Ce projet est soumis à une licence spécifique à l'Institut Curie. Consultez le fichier Logiciel MitoticCellVolume_EB1cometAnalysis.licence pour plus de détails.
README.md at commit b704129, under MIT · at the source
Overview
- Biology of Centrosomes and Genetic Instability team, Institut Curie, Centre National de la Recherche Scientifique (CNRS) Unité Mixte de Recherche 144, Université Paris Sciences et Lettres (PSL Research University), Paris, France
- Cell Migration and Invasion team, Institut Curie, CNRS-UMR144, PSL Research University, Paris, France
- Molecular, Cellular and Development Biology Unit (MCD) – Centre for Integrative Biology (CBI), University of Toulouse, CNRS, Toulouse, France
- Cell and Tissue Imaging Facility (PICT-IBiSA), Institut Curie, CNRS-UMR 144, PSL Research University, Paris, France
- Institut Curie, CNRS-UMR 168, Université Paris Sciences et Lettres, Paris, France
- Aging and Aneuploidy Laboratory, i3S - Instituto de Investigação e Inovação em Saúde, IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal
- Université Paris Cité, CNRS-UMR 8236, Paris, France
Abstract
Research on mitosis has predominantly examined cell-autonomous mechanisms, yet its regulation and consequences within the broader physiological context remain largely unexplored. It remains, therefore, unknown whether and how spindle assembly and chromosome segregation are influenced by tissue properties. We have investigated this question in the mammalian embryonic brain, using high-resolution microscopy combined with pharmacological perturbations and minimal cell systems to break down the contribution of tissue environment to mitotic fidelity. We found that cell density imposes biomechanical constraints upon the apical radial glial (aRG) cell population during their highly proliferative period, which enhances spindle pole microtubule polymerization rates and potentiates chromosome mis-segregation. Mechanistically, we show that cortical tension relying on branched actin organization at the cell cortex conveys mechanical stress exerted by cell density. We identified the microtubule depolymerase MCAK/
Reproduced under the paper's license (CC BY), from the paper cited above.
Repository
Its files are read in the Code ↔ Paper reader above.
Anne-SophieMACE/MitoticCellVolume_EB1cometAnalysis
b70412994435b80a427dc04291f03eb6ef3eec5c, 23 March 2026Availability: 1 check, the latest on 27 September 2026: the link answers
- 27 September 2026: the link answers
1 file
- README.md, Text, 9 lines
The paper's code and data availability statement is in the Data section.
Tracing map
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Data
Data links
- ebi.ac.uk/
biostudies/ , EMBL-EBI; found in the text, “Author contributions”sourcedata
Data availability
The computer codes produced in this study are available in the following databases: Modelling computer scripts : GitHub https://
The source data of this paper are collected in the following database record: biostudies:S-SCDT-10_103
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 15 authors, 4 keywords, 11 MeSH terms, 4 funders, 85 references, 27 RRIDs.
Cite
This paper
Marthiens, V., Jawish, L., Malosse, M., Basto, C., Krndija, D., Macé, A.-S., Laigna, E., Terrizzano, A., Peyret, C., Pan, T., Zajac, O., Perrin, L., Logarinho, E., Villard, C., & Basto, R. (2026). Mechanical constraints regulate embryonic stem cell mitosis in the developing brain. The EMBO journal, 45(16), 5622-5656. https://
BibTeX
@article{marthiens2026me
author = {Marthiens, Véronique and Jawish, Lin and Malosse, Margaux and Basto, Clara and Krndija, Denis and Macé, Anne-Sophie and Laigna, Elmar and Terrizzano, Antonia and Peyret, Corentin and Pan, Teng and Zajac, Olivier and Perrin, Louisiane and Logarinho, Elsa and Villard, Catherine and Basto, Renata},
title = {{Mechanical constraints regulate embryonic stem cell mitosis in the developing brain}},
journal = {The EMBO journal},
year = {2026},
month = jul,
volume = {45},
number = {16},
pages = {5622--5656},
publisher = {Nature Publishing Group},
issn = {0261-4189},
doi = {10.1038/
url = {https://
pmid = {42432253},
pmcid = {PMC13482099}
}
RIS
TY - JOUR
AU - Marthiens, Véronique
AU - Jawish, Lin
AU - Malosse, Margaux
AU - Basto, Clara
AU - Krndija, Denis
AU - Macé, Anne-Sophie
AU - Laigna, Elmar
AU - Terrizzano, Antonia
AU - Peyret, Corentin
AU - Pan, Teng
AU - Zajac, Olivier
AU - Perrin, Louisiane
AU - Logarinho, Elsa
AU - Villard, Catherine
AU - Basto, Renata
TI - Mechanical constraints regulate embryonic stem cell mitosis in the developing brain
T2 - The EMBO journal
J2 - EMBO J
PY - 2026
DA - 2026/
VL - 45
IS - 16
SP - 5622
EP - 5656
SN - 0261-4189
PB - Nature Publishing Group
DO - 10.1038/
UR - https://
LA - en
ER -
CSL-JSON
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