OSCR

Distinct laminar origins of sensory-evoked high-gamma and low-frequency ECoG signals revealed by optogenetics.

Overview

  1. Department of Neuroscience, University of California, Berkeley, CA 94720
  2. Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720
  3. Biological Systems and Engineering Division, Lawrence Berkeley National Lab, Berkeley, CA 94720
  4. Redwood Center for Theoretical Neuroscience, University of California, Berkeley, CA 94720
  5. Scientific Data Division, Lawrence Berkeley National Lab, Berkeley, CA 94720
Institutions: Lawrence Berkeley National Laboratory (United States); University of California System (United States)
Dates: received 20 June 2025; accepted 19 February 2026; published online 1 April 2026; in print 7 April 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1073/pnas.2516293123 · PMID 41920867 · PMCID PMC13056151 · OpenAlex W7146936943
Open access: hybrid, a free copy (OpenAlex)
Status: data only
Categories: EEG (modality), intracranial EEG (iEEG / ECoG / SEEG) (modality), mouse (organism), systems (subfield)
Methods: Connectivity, Statistics, Preprocessing, Spectral & time-frequency, fMRI & imaging, Single-unit activity, calcium imaging
Keywords: electrocorticography, high-gamma, laminar origin, inverse problem, optogenetics
MeSH: Evoked Potentials, Somatosensory*, Gamma Rhythm*, Optogenetics*, Somatosensory Cortex*, Animals, Electroencephalography, Mice, Pyramidal Cells, Vibrissae (* major topic)
Journal subjects: Biological Sciences, Neuroscience
Topic: Neural dynamics and brain function (Cognitive Neuroscience, Neuroscience), according to OpenAlex
Funding: U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) (NS118648, NS092367); LBNL LDRD (Neural Systems and Machine Learning Lab)
Citations: cited by 1 paper (Europe PMC); 82 references in the paper
Research resources: RRID:Addgene_105677

Abstract

Electrocorticography (ECoG) provides a high-spatiotemporal-resolution measure of cortical activity (cortical surface electrical potentials, CSEPs) in humans and animals. The CSEP high-gamma band (Hγ, 65 to 170 Hz) correlates with neuronal firing rates at the columnar spatial scale and is widely used as a biomarker of local activity. Whether Hγ reports all stages of columnar processing, intermediate processing in L2/3 (close to the ECoG electrode), or the main columnar output in L5, is unknown. We disentangled the laminar origins of Hγ and other ECoG bands by optogenetically suppressing L2/3 or L5 pyramidal cells during micro-ECoG recording in the mouse somatosensory cortex. Whisker deflections evoked transient, topographically localized CSEPs. L5 optogenetic suppression most strongly reduced 65 to 450 Hz (Hγ-uHγ) bands in sensory-evoked ECoG signals, whereas L2/3 suppression most strongly reduced 4 to 27 Hz (θ-β) bands. Thus, different CSEP frequency bands reflect layer-specific activity and are biomarkers of distinct stages of sensory-evoked columnar processing.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper links to its data, not to its authors' code: see the Data section.

The paper's code and data availability statement is in the Data section.

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Data

Datasets cited

Data, Materials, and Software Availability

Electrophysiology recordings are deposited in FigShare https://doi.org/10.6084/m9.figshare.31684585 (82).

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 28 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 3 authors, 5 keywords, 9 MeSH terms, 2 funders, 81 references, 1 RRID.

Cite

This paper

Garderes, P.-M., Feldman, D. E., & Bouchard, K. E. (2026). Distinct laminar origins of sensory-evoked high-gamma and low-frequency ECoG signals revealed by optogenetics. Proceedings of the National Academy of Sciences of the United States of America, 123(14), e2516293123. https://doi.org/10.1073/pnas.2516293123

BibTeX

@article{garderes2026distinct,
author = {Garderes, Pierre-Marie and Feldman, Daniel E. and Bouchard, Kristofer E.},
title = {{Distinct laminar origins of sensory-evoked high-gamma and low-frequency ECoG signals revealed by optogenetics}},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
year = {2026},
month = apr,
volume = {123},
number = {14},
pages = {e2516293123},
publisher = {National Academy of Sciences},
issn = {0027-8424},
doi = {10.1073/pnas.2516293123},
url = {https://doi.org/10.1073/pnas.2516293123},
pmid = {41920867},
pmcid = {PMC13056151}
}

RIS

TY - JOUR
AU - Garderes, Pierre-Marie
AU - Feldman, Daniel E.
AU - Bouchard, Kristofer E.
TI - Distinct laminar origins of sensory-evoked high-gamma and low-frequency ECoG signals revealed by optogenetics
T2 - Proceedings of the National Academy of Sciences of the United States of America
J2 - Proc Natl Acad Sci U S A
PY - 2026
DA - 2026/04/01
VL - 123
IS - 14
SP - e2516293123
SN - 0027-8424
PB - National Academy of Sciences
DO - 10.1073/pnas.2516293123
UR - https://doi.org/10.1073/pnas.2516293123
LA - en
ER -

CSL-JSON

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"author": [
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"given": "Pierre-Marie"
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