OSCR

Enhancer RNA-mediated transcriptional regulation of TDP-43 during early neural lineage specification.

Overview

Authors: Yeonju Jang1, Hyohi Lee1, Myunggeun Oh1, Jiin Moon1, Seung-Jin Kim2, Jaeil Han3, Jong-Lyul Park4, Seung-Kyoon Kim1
ORCID iDs: Hyohi Lee, Seung-Kyoon Kim
  1. Department of Convergent Bioscience and Informatics, and Graduate School of Biological Sciences, Chungnam National University, Daejeon, Republic of Korea
  2. Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon, Republic of Korea
  3. Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon, Republic of Korea
  4. Personalized Genomic Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, Republic of Korea
Institutions: Chungnam National University (South Korea); Kangwon National University (South Korea); Korea Research Institute of Bioscience and Biotechnology (South Korea)
Journal: Animal cells and systems, volume 30, issue 1, pages 320-341
Dates: published online 26 March 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1080/19768354.2026.2643998 · PMID 41908332 · PMCID PMC13023014 · OpenAlex W7140938552
Open access: gold, a free copy (OpenAlex)
Status: data only (how the code is found and verified)
Methods: Statistics, fMRI & imaging
Keywords: TDP-43, enhancer RNAs (eRNAs), embryonic stem cells (ESCs), neural lineage specification, epigenetics
Topic: Neurogenesis and neuroplasticity mechanisms (Developmental Neuroscience, Neuroscience), according to OpenAlex
Funding: Bio&Medical Technology Development Program (RS-2025-02305916); Basic Science Research Program (RS-2023-00209842); Singapore National Research Foundation; National Research Council of Science and Technology (CRC22014-100)
Citations: not cited yet (Europe PMC); 78 references in the paper

Abstract

TAR DNA-binding protein 43 (TDP-43) is a DNA- and RNA-binding protein that regulates gene expression by modulating transcription and RNA processing. It plays pivotal roles in neuronal development and function, and its mislocalization and aggregation are major pathological features of several neurodegenerative diseases. However, the regulatory mechanisms that control Tdp-43 expression and activity during the transition from embryonic stem cells (ESCs) to neural progenitor cells (NPCs) remain poorly understood. Through integrative epigenomic and transcriptomic analyses, we identified multiple intergenic and intragenic enhancers within and around the Tdp-43 locus that generate enhancer RNAs (eRNAs). These eRNAs exhibit dynamic, region-specific expression changes and modulate Tdp-43 transcription in a stage- and context-dependent manner. Specifically, a subset of eRNAs was highly expressed in ESCs and downregulated upon differentiation, while others were selectively retained or induced in NPCs, paralleling changes in enhancer usage and histone modification states. Targeted knockdown of these eRNAs decreased Tdp-43 expression and was accompanied by changes in the expression of pluripotency- and lineage-associated markers, without implying direct control over full differentiation trajectories. These findings uncover a previously unrecognized aspect of Tdp-43 transcriptional regulation and highlight the significance of enhancer dynamics in the epigenetic regulation of TDP-43 expression during early lineage specification.

Reproduced under the paper's license (CC BY), from the paper cited above.

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Data

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Version 1, 30 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 8 authors, 5 keywords, 4 funders, 78 references.

Cite

This paper

Jang, Y., Lee, H., Oh, M., Moon, J., Kim, S.-J., Han, J., Park, J.-L., & Kim, S.-K. (2026). Enhancer RNA-mediated transcriptional regulation of TDP-43 during early neural lineage specification. Animal cells and systems, 30(1), 320-341. https://doi.org/10.1080/19768354.2026.2643998

BibTeX

@article{jang2026enhancer,
author = {Jang, Yeonju and Lee, Hyohi and Oh, Myunggeun and Moon, Jiin and Kim, Seung-Jin and Han, Jaeil and Park, Jong-Lyul and Kim, Seung-Kyoon},
title = {{Enhancer RNA-mediated transcriptional regulation of TDP-43 during early neural lineage specification}},
journal = {Animal cells and systems},
year = {2026},
month = mar,
volume = {30},
number = {1},
pages = {320--341},
publisher = {Taylor \& Francis},
issn = {1976-8354},
doi = {10.1080/19768354.2026.2643998},
url = {https://doi.org/10.1080/19768354.2026.2643998},
pmid = {41908332},
pmcid = {PMC13023014}
}

RIS

TY - JOUR
AU - Jang, Yeonju
AU - Lee, Hyohi
AU - Oh, Myunggeun
AU - Moon, Jiin
AU - Kim, Seung-Jin
AU - Han, Jaeil
AU - Park, Jong-Lyul
AU - Kim, Seung-Kyoon
TI - Enhancer RNA-mediated transcriptional regulation of TDP-43 during early neural lineage specification
T2 - Animal cells and systems
J2 - Anim Cells Syst (Seoul)
PY - 2026
DA - 2026/03/26
VL - 30
IS - 1
SP - 320
EP - 341
SN - 1976-8354
PB - Taylor & Francis
DO - 10.1080/19768354.2026.2643998
UR - https://doi.org/10.1080/19768354.2026.2643998
LA - en
ER -

CSL-JSON

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