Ankyrin-B and ankyrin-G are required for synaptic integrity in the mouse outer retina.
Overview
- Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
- Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, WI 53705, USA
- Department of Ophthalmology and Stein Eye Institute, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA
- Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA
Abstract
Retinal circuit assembly relies on the precise timing and positioning of key molecules between neuronal partners to mediate proper synapse formation. In the outer retina, horizontal cells (HCs) are important interneurons that make the first contacts with photoreceptors and begin to segregate visual information into two distinct pathways by selectively forming synapses to the different types of photoreceptors. Dendrites of HCs synapse exclusively to cone photoreceptors, whereas the axon terminal synapses to rod photoreceptors. Failure to properly form these early connections disrupts the downstream connectivity of other postsynaptic neurons and leads to abnormal visual function. Although these early events are critical for proper synapse development, little is known about how this process is coordinated in the outer retina. In the present study, we performed single-cell RNA sequencing and uncovered members of the cytoskeletal scaffolding family of ankyrins to be differentially expressed in HCs during development. Specifically, we found ankyrin-B to be highly expressed in HCs at early time points and ankyrin-G to be expressed at later stages. Genetic deletion of both ankyrin-B and ankyrin-G disrupts outer retinal synaptic integrity and leads to impaired in vivo retinal responses. In summary, our findings uncovered a new requirement for ankyrin-B and ankyrin-G in maintaining synaptic connectivity in the outer retina essential for normal visual function.
Reproduced under the paper's license (CC BY-NC), from the paper cited above.
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Data
Datasets cited
- geo:GSE336188 — at NCBI GEO; found in “Data Availability”
- zenodo:20707795 — at Zenodo; found in “Data Availability”
Data Availability
The scRNA-Seq data generated in this study have been deposited to the Gene Expression Omnibus (GEO) under accession ID GSE336188 (https://
Reproduced under the paper's license (CC BY-NC), from the paper cited above.
Versions
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Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 14 authors, 2 funders, 57 references.
Cite
This paper
Perez, R. M., Campbell, J., Cavallini, M., Todora, C., Becerril, D., Goswami-Sewell, D., Venkatraman, R., Gomez, C. C., Bagnetto, C., Lee, A., Mattos, M. F., Peng, Y.-R., Hoon, M., & Zuniga-Sanchez, E. (2026). Ankyrin-B and ankyrin-G are required for synaptic integrity in the mouse outer retina. PNAS nexus, 5(7), pgag239. https://
BibTeX
@article{perez2026ankyri
author = {Perez, Ross M and Campbell, Jay and Cavallini, Martina and Todora, Collin and Becerril, Daniela and Goswami-Sewell, Debalina and Venkatraman, Rajashree and Gomez, Cesiah C and Bagnetto, Caitlin and Lee, Audrey and Mattos, Marlon F and Peng, Yi-Rong and Hoon, Mrinalini and Zuniga-Sanchez, Elizabeth},
title = {{Ankyrin-B and ankyrin-G are required for synaptic integrity in the mouse outer retina}},
journal = {PNAS nexus},
year = {2026},
month = jul,
volume = {5},
number = {7},
pages = {pgag239},
publisher = {Oxford University Press},
issn = {2752-6542},
doi = {10.1093/
url = {https://
pmid = {42491609},
pmcid = {PMC13378166}
}
RIS
TY - JOUR
AU - Perez, Ross M
AU - Campbell, Jay
AU - Cavallini, Martina
AU - Todora, Collin
AU - Becerril, Daniela
AU - Goswami-Sewell, Debalina
AU - Venkatraman, Rajashree
AU - Gomez, Cesiah C
AU - Bagnetto, Caitlin
AU - Lee, Audrey
AU - Mattos, Marlon F
AU - Peng, Yi-Rong
AU - Hoon, Mrinalini
AU - Zuniga-Sanchez, Elizabeth
TI - Ankyrin-B and ankyrin-G are required for synaptic integrity in the mouse outer retina
T2 - PNAS nexus
J2 - PNAS Nexus
PY - 2026
DA - 2026/
VL - 5
IS - 7
SP - pgag239
SN - 2752-6542
PB - Oxford University Press
DO - 10.1093/
UR - https://
LA - en
ER -
CSL-JSON
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