OSCR

Ankyrin-B and ankyrin-G are required for synaptic integrity in the mouse outer retina.

Overview

Authors: Ross M Perez1, Jay Campbell2, Martina Cavallini3, Collin Todora1, Daniela Becerril1, Debalina Goswami-Sewell1, Rajashree Venkatraman1, Cesiah C Gomez1, Caitlin Bagnetto1, Audrey Lee1, Marlon F Mattos1, Yi-Rong Peng3, Mrinalini Hoon2, Elizabeth Zuniga-Sanchez1,4
ORCID iDs: Jay Campbell, Martina Cavallini, Debalina Goswami-Sewell, Caitlin Bagnetto, Yi-Rong Peng, Mrinalini Hoon, Elizabeth Zuniga-Sanchez
  1. Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
  2. Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, WI 53705, USA
  3. Department of Ophthalmology and Stein Eye Institute, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA
  4. Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA
Institutions: Baylor College of Medicine (United States); University of Wisconsin–Madison (United States); University of California, Los Angeles (United States)
Journal: PNAS nexus, volume 5, issue 7, article pgag239
Dates: received 26 May 2026; accepted 29 June 2026; published online 9 July 2026
Type: Research article · Language: English
License: CC BY-NC
Identifiers: DOI 10.1093/pnasnexus/pgag239 · PMID 42491609 · PMCID PMC13378166 · OpenAlex W7167793589
Open access: gold, a free copy (OpenAlex)
Status: data only (how the code is found and verified)
Categories: mouse (organism), cellular / molecular (subfield)
Topic: Retinal Development and Disorders (Ophthalmology, Medicine), according to OpenAlex
Funding: NEI NIH HHS (R01 EY033037, F31 EY035931, R01 EY035324, T32 EY007026, P30 EY000331, P30 EY002520); NIGMS NIH HHS (T32 GM008042)
Citations: not cited yet (Europe PMC); 57 references in the paper

Abstract

Retinal circuit assembly relies on the precise timing and positioning of key molecules between neuronal partners to mediate proper synapse formation. In the outer retina, horizontal cells (HCs) are important interneurons that make the first contacts with photoreceptors and begin to segregate visual information into two distinct pathways by selectively forming synapses to the different types of photoreceptors. Dendrites of HCs synapse exclusively to cone photoreceptors, whereas the axon terminal synapses to rod photoreceptors. Failure to properly form these early connections disrupts the downstream connectivity of other postsynaptic neurons and leads to abnormal visual function. Although these early events are critical for proper synapse development, little is known about how this process is coordinated in the outer retina. In the present study, we performed single-cell RNA sequencing and uncovered members of the cytoskeletal scaffolding family of ankyrins to be differentially expressed in HCs during development. Specifically, we found ankyrin-B to be highly expressed in HCs at early time points and ankyrin-G to be expressed at later stages. Genetic deletion of both ankyrin-B and ankyrin-G disrupts outer retinal synaptic integrity and leads to impaired in vivo retinal responses. In summary, our findings uncovered a new requirement for ankyrin-B and ankyrin-G in maintaining synaptic connectivity in the outer retina essential for normal visual function.

Reproduced under the paper's license (CC BY-NC), from the paper cited above.

Code

The paper links to its data and to no code of its authors: see the Data section.

Tracing map

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Data

Datasets cited

Data Availability

The scRNA-Seq data generated in this study have been deposited to the Gene Expression Omnibus (GEO) under accession ID GSE336188 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336188) and Zenodo (10.5281/zenodo.20707795 (https://doi.org/10.5281/zenodo.20707795)).

Reproduced under the paper's license (CC BY-NC), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 14 authors, 2 funders, 57 references.

Cite

This paper

Perez, R. M., Campbell, J., Cavallini, M., Todora, C., Becerril, D., Goswami-Sewell, D., Venkatraman, R., Gomez, C. C., Bagnetto, C., Lee, A., Mattos, M. F., Peng, Y.-R., Hoon, M., & Zuniga-Sanchez, E. (2026). Ankyrin-B and ankyrin-G are required for synaptic integrity in the mouse outer retina. PNAS nexus, 5(7), pgag239. https://doi.org/10.1093/pnasnexus/pgag239

BibTeX

@article{perez2026ankyrin,
author = {Perez, Ross M and Campbell, Jay and Cavallini, Martina and Todora, Collin and Becerril, Daniela and Goswami-Sewell, Debalina and Venkatraman, Rajashree and Gomez, Cesiah C and Bagnetto, Caitlin and Lee, Audrey and Mattos, Marlon F and Peng, Yi-Rong and Hoon, Mrinalini and Zuniga-Sanchez, Elizabeth},
title = {{Ankyrin-B and ankyrin-G are required for synaptic integrity in the mouse outer retina}},
journal = {PNAS nexus},
year = {2026},
month = jul,
volume = {5},
number = {7},
pages = {pgag239},
publisher = {Oxford University Press},
issn = {2752-6542},
doi = {10.1093/pnasnexus/pgag239},
url = {https://doi.org/10.1093/pnasnexus/pgag239},
pmid = {42491609},
pmcid = {PMC13378166}
}

RIS

TY - JOUR
AU - Perez, Ross M
AU - Campbell, Jay
AU - Cavallini, Martina
AU - Todora, Collin
AU - Becerril, Daniela
AU - Goswami-Sewell, Debalina
AU - Venkatraman, Rajashree
AU - Gomez, Cesiah C
AU - Bagnetto, Caitlin
AU - Lee, Audrey
AU - Mattos, Marlon F
AU - Peng, Yi-Rong
AU - Hoon, Mrinalini
AU - Zuniga-Sanchez, Elizabeth
TI - Ankyrin-B and ankyrin-G are required for synaptic integrity in the mouse outer retina
T2 - PNAS nexus
J2 - PNAS Nexus
PY - 2026
DA - 2026/07/09
VL - 5
IS - 7
SP - pgag239
SN - 2752-6542
PB - Oxford University Press
DO - 10.1093/pnasnexus/pgag239
UR - https://doi.org/10.1093/pnasnexus/pgag239
LA - en
ER -

CSL-JSON

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