Diverse ancestral myosin motors generate and segregate distinct types of nanocluster-rich domains at the plasma membrane.

Overview

Authors: Parijat Sil¹, Thomas S van Zanten^1,2, Sowmya Jahnavi^1,3, Ajay Bansal⁴, Hafez Razmazma^3,5, Suvrajit Saha¹, Bhagyashri Mahajan^1,3,6, Mukesh Kumar⁷, Phillip J Stansfeld^3,5, Parvinder Pal Singh⁷, Madan Rao⁴, Satyajit Mayor^1,3

ORCID iDs: Parijat Sil, Thomas S van Zanten, Sowmya Jahnavi, Hafez Razmazma, Suvrajit Saha, Bhagyashri Mahajan, Phillip J Stansfeld, Madan Rao, Satyajit Mayor

National Centre for Biological Sciences, Bangalore, India
Fundación Agencia Aragonesa para la Investigación y el Desarrollo (ARAID), Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-Universidad de Zaragoza and CIBER-BBN, Zaragoza, Spain
Warwick Medical School, University of Warwick, Coventry, UK
Simons Centre for the Study of Living Machines, National Centre for Biological Sciences, Bangalore, India
School of Life Sciences and Department of Chemistry, University of Warwick, Coventry, UK
Trans-disciplinary University, Bangalore, India
CSIR–Indian Institute of Integrative Medicine, Jammu, India

Institutions: National Centre for Biological Sciences (India); Universidad de Zaragoza (Spain); Fundacion Agencia Aragonesa para la Investigacion y el Desarrollo (Spain); Instituto de Nanociencia y Materiales de Aragón (Spain); University of Warwick (United Kingdom); Indian Institute of Integrative Medicine (India); Council of Scientific and Industrial Research (India)

Journal: Science advances, volume 12, issue 37, article eaeb8889

Dates: received 12 September 2025; accepted 4 August 2026; published online 11 September 2026; in print September 2026

Type: Research article · Language: English

License: CC BY

Identifiers: DOI 10.1126/sciadv.aeb8889 · PMID 42726867 · PMCID PMC13564907 · OpenAlex W7212262442

Open access: gold, a free copy (OpenAlex)

Status: dead link

Categories: cellular / molecular (subfield)

Methods: Statistics, Evoked potentials, Connectivity, fMRI & imaging

MeSH: Cell Membrane*, Membrane Microdomains*, Myosins*, Actin Cytoskeleton, Animals (* major topic)

Topic: Lipid Membrane Structure and Behavior (Molecular Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex

Funding: Wellcome Trust

Citations: not cited yet (Europe PMC); 72 references in the paper

Abstract

Molecular organization of the plasma membrane at nano- and micrometer scales is critical for its function in all living cells. This emerges not only from the self-assembly of lipids and proteins but also from active forces originating in the underlying cytoskeletal cortex. These forces drive membrane molecules into nonequilibrium steady-state patterns such as nanoclusters. However, the molecular agents connecting membrane organization with cytoskeletal dynamics and stresses have remained unknown. Here, we show that two classes of ubiquitous ancestral nonmuscle myosins are deployed for the organization of different types of membrane components. Inner leaflet–localized class I myosins link outerleaflet glycosylphosphatidylinositol-anchored molecules to juxta-membrane actin filaments, whereas the more cortically localized Class II myosins operate on transmembrane proteins endowed with actin-binding capacity. Consistent with an active Flory-Huggins theory for phase separation, these observations show that the distinct motor-driven membrane molecules generate spatially segregated mesoscale domains, enriched in nanoclusters derived from different myosin classes. Moreover, chemically reversible posttranslational modifications such as palmitoylation enable concatenation of these domains by enhancing the affinity of the membrane domain constituents for each other. We anticipate that the segregation potential of the adenosine 5′-triphosphate (ATP)–fueled cell membrane is made available for the crucial purpose of modulating information transduction because it can be regulated in space and time during the construction of signaling cascades, underpinning functional plasma membrane organization.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

No file of the authors' code could be read here: it is described below, and read at its source.

Zenodo 21241290

License: none: the authors keep all their rights

State: the link is dead, verified on 26 September 2026

Evidence: found in the paper

Software Heritage: not checked

Found in: “Data, code, and materials availability:”

Not found: README, license file, CITATION.cff, environment file, tests, continuous integration, documentation

Availability: 1 check, the latest on 26 September 2026: the link is dead (HTTP 404)

26 September 2026: the link is dead (HTTP 404)

At the source:

The paper's code and data availability statement is in the Data section.

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Data

Datasets cited

zenodo:21217537 — at Zenodo; found in “Statistics and data reproducibility”

Data, code, and materials availability

All data and code needed to evaluate and reproduce the results in the paper are present in the paper and/or the Supplementary Materials and in the Zenodo repositories 10.5281/zenodo.21217537 and 10.5281/zenodo.21241290. The constructs, reagents, and cell lines can be provided by the National Centre for Biological Sciences pending scientific review and a completed material transfer agreement with the National Centre for Biological Sciences. Requests for the constructs, reagents, and cell lines should be submitted to S.M. at .

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

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Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 12 authors, 5 MeSH terms, 1 funder, 66 references.

Cite

This paper

Sil, P., van Zanten, T. S., Jahnavi, S., Bansal, A., Razmazma, H., Saha, S., Mahajan, B., Kumar, M., Stansfeld, P. J., Singh, P. P., Rao, M., & Mayor, S. (2026). Diverse ancestral myosin motors generate and segregate distinct types of nanocluster-rich domains at the plasma membrane. Science advances, 12(37), eaeb8889. https://doi.org/10.1126/sciadv.aeb8889

BibTeX

@article{sil2026diverse, author = {Sil, Parijat and van Zanten, Thomas S and Jahnavi, Sowmya and Bansal, Ajay and Razmazma, Hafez and Saha, Suvrajit and Mahajan, Bhagyashri and Kumar, Mukesh and Stansfeld, Phillip J and Singh, Parvinder Pal and Rao, Madan and Mayor, Satyajit}, title = {{Diverse ancestral myosin motors generate and segregate distinct types of nanocluster-rich domains at the plasma membrane}}, journal = {Science advances}, year = {2026}, month = sep, volume = {12}, number = {37}, pages = {eaeb8889}, publisher = {American Association for the Advancement of Science}, issn = {2375-2548}, doi = {10.1126/sciadv.aeb8889}, url = {https://doi.org/10.1126/sciadv.aeb8889}, pmid = {42726867}, pmcid = {PMC13564907} }

RIS

TY - JOUR AU - Sil, Parijat AU - van Zanten, Thomas S AU - Jahnavi, Sowmya AU - Bansal, Ajay AU - Razmazma, Hafez AU - Saha, Suvrajit AU - Mahajan, Bhagyashri AU - Kumar, Mukesh AU - Stansfeld, Phillip J AU - Singh, Parvinder Pal AU - Rao, Madan AU - Mayor, Satyajit TI - Diverse ancestral myosin motors generate and segregate distinct types of nanocluster-rich domains at the plasma membrane T2 - Science advances J2 - Sci Adv PY - 2026 DA - 2026/09/11 VL - 12 IS - 37 SP - eaeb8889 SN - 2375-2548 PB - American Association for the Advancement of Science DO - 10.1126/sciadv.aeb8889 UR - https://doi.org/10.1126/sciadv.aeb8889 LA - en ER -

CSL-JSON

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