Reconstitution of actomyosin networks in cell-sized liposomes dissects distinct mechanisms of membrane blebbing and symmetry breaking.
The 2 matches
- [1] § MATERIALS AND METHODS › Agent-based model ↔ header.h, lines 441–500 · score 0.74 · thermal fluctuation, cylindrical segments, repulsive forces, displacements, walk, assembled
- [2] § MATERIALS AND METHODS › Agent-based model ↔ dataMgr.c, lines 707–774 · score 0.57 · thermal fluctuation, barbed end, nucleation, assembly, ACPs, bind
Paper
Loaded from Europe PMC by your browser, not stored by OSCR: doi.org · Europe PMC
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The authors' code
C/C++ header · 1,063 lines · 46 KB · no license · 1 match
header.h at commit 707dbea, no license · at the source
Overview
- RIKEN Center for Integrative Medical Sciences, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
- RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan
- Graduate School of Medicine, Science and Technology, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
- PRESTO, JST, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan
- Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907-2032, USA
- EMBRIO Institute, Purdue University, West Lafayette, IN 47907-2032, USA
- Faculty of Science and Technology, Keio University, Kohoku-ku, Yokohama, Kanagawa 223-0061, Japan
Abstract
The actin cortex, a thin layer of actomyosin network beneath the plasma membrane, regulates various cell functions by generating active forces and inducing membrane deformations, including blebs. Although upstream signaling is involved in regulating cell shape, the extent to which downstream actomyosin molecules can control the shape remains elusive. Here, using a minimal reconstituted system combined with an agent-based computational model, we show that actin-membrane coupling strength determines the magnitude of membrane deformation, while its balance with actin network connectivity governs the bleb initiation mechanism, either by detachment of the cortex from the membrane or by rupture of the cortex. This balance also modulates single versus multiple bleb formation, thereby regulating symmetry breaking. Furthermore, our results suggest that not only the dense cortical network but also the sparse volume-spanning network actively contributes to the regulation of bleb number. These findings provide mechanistic insights into how cells tune actin network organization to control their shape and polarity.
Reproduced under the paper's license (CC BY-NC), from the paper cited above.
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Its files are read in the Code ↔ Paper reader above, with 2 matches between paragraphs and lines of code.
ktyman2/CellBleb
707dbea3af825df2bec27deb4e63dbc1ae487edd, 6 April 2026Availability: 1 check, the latest on 27 September 2026: the link answers
- 27 September 2026: the link answers
20 files, not copied: shown from their source
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- bead.c — C, 527 lines, shown from its source
- boundary.c — C, 621 lines, shown from its source
- calForce.c — C, 898 lines, shown from its source
- common.c — C, 423 lines, shown from its source
- dataMgr.c — C, 2,832 lines, 1 match, shown from its source
- divConfPV.c — C, 78 lines, shown from its source
- divConfVmd.c — C, 74 lines, shown from its source
- error.c — C, 672 lines, shown from its source
- gatPrint.c — C, 542 lines, shown from its source
- header.h — C/C++, 1,063 lines, 1 match, shown from its source
- init.c — C, 2,109 lines, shown from its source
- main.c — C, 60 lines, shown from its source
- membrane.c — C, 2,119 lines, shown from its source
- paraProc.c — C, 3,275 lines, shown from its source
- process.c — C, 756 lines, shown from its source
- record.c — C, 5,331 lines, shown from its source
- rheology.c — C, 510 lines, shown from its source
- rng.c — C, 227 lines, shown from its source
- tools.c — C, 1,185 lines, shown from its source
- update.c — C, 2,825 lines, shown from its source
Zenodo 19435449
Availability: 1 check, the latest on 27 September 2026: the link answers (HTTP 200)
- 27 September 2026: the link answers (HTTP 200)
20 files
- bead.c — C, 527 lines
- boundary.c — C, 621 lines
- calForce.c — C, 898 lines
- common.c — C, 423 lines
- dataMgr.c — C, 2,832 lines
- divConfPV.c — C, 78 lines
- divConfVmd.c — C, 74 lines
- error.c — C, 672 lines
- gatPrint.c — C, 542 lines
- header.h — C/C++, 1,063 lines
- init.c — C, 2,109 lines
- main.c — C, 60 lines
- membrane.c — C, 2,119 lines
- paraProc.c — C, 3,275 lines
- process.c — C, 756 lines
- record.c — C, 5,331 lines
- rheology.c — C, 510 lines
- rng.c — C, 227 lines
- tools.c — C, 1,185 lines
- update.c — C, 2,825 lines
The paper's code and data availability statement is in the Data section.
Tracing map
Proposed by the machine: these links were found in the paper and verified at the source, without human review. The map will receive a Zenodo DOI once one of the paper's authors has validated it with their ORCID.
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Data
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Data, code, and materials availability
All relevant codes for the agent-based model simulation can be found on the GitHub repository: https://
Reproduced under the paper's license (CC BY-NC), from the paper cited above.
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Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 3 authors, 7 MeSH terms, 9 funders, 84 references.
Cite
This paper
Miyazaki, M., Laboni, F. S., & Kim, T. (2026). Reconstitution of actomyosin networks in cell-sized liposomes dissects distinct mechanisms of membrane blebbing and symmetry breaking. Science advances, 12(31), eaed8818. https://
BibTeX
@article{miyazaki2026rec
author = {Miyazaki, Makito and Laboni, Fahmida Sultana and Kim, Taeyoon},
title = {{Reconstitution of actomyosin networks in cell-sized liposomes dissects distinct mechanisms of membrane blebbing and symmetry breaking}},
journal = {Science advances},
year = {2026},
month = jul,
volume = {12},
number = {31},
pages = {eaed8818},
publisher = {American Association for the Advancement of Science},
issn = {2375-2548},
doi = {10.1126/
url = {https://
pmid = {42525777},
pmcid = {PMC13440360}
}
RIS
TY - JOUR
AU - Miyazaki, Makito
AU - Laboni, Fahmida Sultana
AU - Kim, Taeyoon
TI - Reconstitution of actomyosin networks in cell-sized liposomes dissects distinct mechanisms of membrane blebbing and symmetry breaking
T2 - Science advances
J2 - Sci Adv
PY - 2026
DA - 2026/
VL - 12
IS - 31
SP - eaed8818
SN - 2375-2548
PB - American Association for the Advancement of Science
DO - 10.1126/
UR - https://
LA - en
ER -
CSL-JSON
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