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Effects of gut microbiota on the susceptibility of ischemic stroke in mice.

Overview

Authors: Guangyan Wu1, Huidi Wang1, Qianyi Zhou1, Jingxiang Fu1, Feng Zhang1, Zhuo Duan1, Shuo Wang1, Jielin Huang1, Hongwei Zhou1, Zhenchao Ma2, Yan He1,3,4,5, Jia Yin6, Kaiyu Xu1
ORCID iDs: Guangyan Wu, Jingxiang Fu, Zhenchao Ma, Yan He, Jia Yin, Kaiyu Xu
  1. Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China
  2. Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou, China
  3. Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou, China
  4. State Key Laboratory of Muti-organ Injury Prevention and Treatment, Southern Medical University, Guangzhou, China
  5. Key Laboratory of Mental Health of the Ministry of Education, Guangzhou, China
  6. Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China
Institutions: Zhujiang Hospital (China); Southern Medical University (China); Zhejiang Chinese Medical University (China); Huzhou Central Hospital (China); Nanfang Hospital (China)
Journal: mSystems, volume 11, issue 8, pages e00149-26
Dates: received 15 February 2026; accepted 22 May 2026; published online 10 July 2026; in print August 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1128/msystems.00149-26 · PMID 42429658 · PMCID PMC13483375 · OpenAlex W7167958667
Open access: gold, a free copy (OpenAlex)
Status: data only
Categories: genetics / omics (modality), mouse (organism), stroke (population)
Methods: Spectral & time-frequency, Statistics, Smoothing, state filtering, decompositions, Machine learning, Connectivity
Keywords: gut microbiota, ischemic stroke, susceptibility, fecal microbiota transplantation
MeSH: Brain Ischemia*, Gastrointestinal Microbiome*, Ischemic Stroke*, Animals, Disease Models, Animal, Disease Susceptibility, Dysbiosis, Fecal Microbiota Transplantation, Male, Metabolomics, Mice, Mice, Inbred C57BL, RNA, Ribosomal, 16S (* major topic)
Topic: Gut microbiota and health (Molecular Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: National Key R&D Program of China (2019YFA0802301); Guangzhou Key Research Program on Brain Science (202206060001); National Natural Science Foundation of China (82272391); National Key R&D Program of China (2019YFA0802301); Guangdong Basic and Applied Basic Research Foundation (2024B1515230009)
Citations: not cited yet (Europe PMC); 56 references in the paper

Abstract

Ischemic stroke is a highly prevalent disease with limited therapeutic options, and emerging evidence suggests that the gut microbiota influences stroke pathophysiology. However, whether the gut microbiota affects individual susceptibility to ischemic stroke remains unclear. Here, middle cerebral artery occlusion was performed to stratify mice into ischemic stroke-sensitive (SEN: reaching humane endpoints within day 1) and ischemic stroke-resistant (RES: surviving to day 7) groups based on post-stroke survival. SEN mice exhibited more severe brain injury than RES mice, accompanied by increased systemic inflammation and elevated intestinal permeability. Fecal microbiota from SEN or RES donors was transplanted into antibiotic-treated recipients. Mice receiving SEN microbiota developed significantly worse outcomes compared with those receiving RES microbiota. Multi-omic analyses of cecal contents, including 16S rRNA gene sequencing and liquid chromatography-mass spectrometry/mass spectrometry-based untargeted metabolomics, identified reduced butyrate-producing bacteria and altered glutathione metabolism as potential contributors to stroke susceptibility. Collectively, these findings demonstrate that gut microbiota and microbial metabolites modulate susceptibility to ischemic stroke.

IMPORTANCE: The role of the gut microbiota in determining susceptibility to ischemic stroke has remained poorly defined. This study demonstrates that microbiota dysbiosis and metabolite alterations functionally increase vulnerability to stroke injury, highlighting the gut microbiome as a potential target for risk stratification and preventive interventions. Modulating the gut microbiota may therefore represent a novel strategy for reducing stroke susceptibility.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper links to its data, not to its authors' code: see the Data section.

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Data

Datasets cited

Data availability

The raw 16S rRNA gene sequencing data have been deposited in the Sequence Read Archive under accession number PRJNA1131626 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1131626). The raw untargeted metabolomic data and processed peak tables are available in MetaboLights repository under accession MTBLS10583 (https://www.ebi.ac.uk/metabolights/MTBLS10583). The metadata for the 16S rRNA gene sequencing and untargeted metabolomic data sets are publicly available on Zenodo at https://zenodo.org/records/20685591.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 13 authors, 4 keywords, 13 MeSH terms, 5 funders, 56 references.

Cite

This paper

Wu, G., Wang, H., Zhou, Q., Fu, J., Zhang, F., Duan, Z., Wang, S., Huang, J., Zhou, H., Ma, Z., He, Y., Yin, J., & Xu, K. (2026). Effects of gut microbiota on the susceptibility of ischemic stroke in mice. mSystems, 11(8), e00149-26. https://doi.org/10.1128/msystems.00149-26

BibTeX

@article{wu2026effects,
author = {Wu, Guangyan and Wang, Huidi and Zhou, Qianyi and Fu, Jingxiang and Zhang, Feng and Duan, Zhuo and Wang, Shuo and Huang, Jielin and Zhou, Hongwei and Ma, Zhenchao and He, Yan and Yin, Jia and Xu, Kaiyu},
title = {{Effects of gut microbiota on the susceptibility of ischemic stroke in mice}},
journal = {mSystems},
year = {2026},
month = jul,
volume = {11},
number = {8},
pages = {e00149--26},
publisher = {American Society for Microbiology (ASM)},
issn = {2379-5077},
doi = {10.1128/msystems.00149-26},
url = {https://doi.org/10.1128/msystems.00149-26},
pmid = {42429658},
pmcid = {PMC13483375}
}

RIS

TY - JOUR
AU - Wu, Guangyan
AU - Wang, Huidi
AU - Zhou, Qianyi
AU - Fu, Jingxiang
AU - Zhang, Feng
AU - Duan, Zhuo
AU - Wang, Shuo
AU - Huang, Jielin
AU - Zhou, Hongwei
AU - Ma, Zhenchao
AU - He, Yan
AU - Yin, Jia
AU - Xu, Kaiyu
TI - Effects of gut microbiota on the susceptibility of ischemic stroke in mice
T2 - mSystems
J2 - mSystems
PY - 2026
DA - 2026/07/10
VL - 11
IS - 8
SP - e00149
EP - 26
SN - 2379-5077
PB - American Society for Microbiology (ASM)
DO - 10.1128/msystems.00149-26
UR - https://doi.org/10.1128/msystems.00149-26
LA - en
ER -

CSL-JSON

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