Loss of the endoplasmic reticulum protein canopy 1 disrupts the function and circuit organization of V2R-expressing vomeronasal sensory neurons.
Overview
Abstract
The vomeronasal organ (VNO) is a specialized chemosensory structure that detects chemical cues involved in predator avoidance, social interactions and reproductive behaviors. In mice, the VNO contains distinct vomeronasal sensory neurons (VSNs) that express vomeronasal receptors (VRs) and formyl peptide receptors. Apical VSNs express Meis2, V1Rs and Gαi2, whereas basal VSNs express Tfap2e (also known as AP-2ε), V2Rs and Gαo. Type 2 VRs (V2Rs) are classified into families A-E, and basal neurons co-express a family C receptor with a VR from another family. Single-cell RNA-sequencing identified ∼980 genes differentially expressed between V1R- and V2R-expressing neurons, many of which are linked to endoplasmic reticulum (ER) functions. Notably, canopy 1 (Cnpy1) is highly enriched in V2R-expressing neurons. The VNO of Cnpy1 knockout mice develops normally but undergoes progressive loss of V2R-expressing neurons, which is associated with increased ER stress gene expression, upregulation of family C V2R mRNAs and reduced V2R protein levels. V2R-expressing VSNs in Cnpy1 knockouts fail to respond to pheromones, show altered guidance and adhesion gene expression, and display disrupted connectivity with the accessory olfactory bulb. These findings emphasize the role of cell-specific ER protein repertoires in maintaining neuronal function.
Reproduced under the paper's license (CC BY), from the paper cited above.
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Data
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- geo:GSE310235 — at NCBI GEO; found in the text, “Footnotes”
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Version 2, 28 September 2026
- Publisher: — → The Company of Biologists
Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 7 authors, 6 keywords, 7 MeSH terms, 7 funders, 64 references.
Cite
This paper
Mathias, N. A., Dolphin, N. M., LeFever, N. M., Amato, E., Ybanez, C. S., Ruquet, A., & Forni, P. E. (2026). Loss of the endoplasmic reticulum protein canopy 1 disrupts the function and circuit organization of V2R-expressing vomeronasal sensory neurons. Development (Cambridge, England), 153(12), dev205386. https://
BibTeX
@article{mathias2026loss
author = {Mathias, Nicholas A and Dolphin, Nikki M and LeFever, Noah M and Amato, Enrico and Ybanez, Carlsy S and Ruquet, Anthony and Forni, Paolo E},
title = {{Loss of the endoplasmic reticulum protein canopy 1 disrupts the function and circuit organization of V2R-expressing vomeronasal sensory neurons}},
journal = {Development (Cambridge, England)},
year = {2026},
month = jun,
volume = {153},
number = {12},
pages = {dev205386},
publisher = {The Company of Biologists},
issn = {0950-1991},
doi = {10.1242/
url = {https://
pmid = {42299035},
pmcid = {PMC13354959}
}
RIS
TY - JOUR
AU - Mathias, Nicholas A
AU - Dolphin, Nikki M
AU - LeFever, Noah M
AU - Amato, Enrico
AU - Ybanez, Carlsy S
AU - Ruquet, Anthony
AU - Forni, Paolo E
TI - Loss of the endoplasmic reticulum protein canopy 1 disrupts the function and circuit organization of V2R-expressing vomeronasal sensory neurons
T2 - Development (Cambridge, England)
J2 - Development
PY - 2026
DA - 2026/
VL - 153
IS - 12
SP - dev205386
SN - 0950-1991
PB - The Company of Biologists
DO - 10.1242/
UR - https://
LA - en
ER -
CSL-JSON
{
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"container-title": "Development (Cambridge, England)",
"author": [
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"family": "Mathias",
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"URL": "https://
"language": "en",
"issued": {
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