OSCR

Loss of the endoplasmic reticulum protein canopy 1 disrupts the function and circuit organization of V2R-expressing vomeronasal sensory neurons.

Overview

Authors: Nicholas A Mathias1, Nikki M Dolphin1, Noah M LeFever1, Enrico Amato Jr1, Carlsy S Ybanez1, Anthony Ruquet1, Paolo E Forni1
ORCID iDs: Nicholas A Mathias, Nikki M Dolphin, Noah M LeFever, Enrico Amato Jr, Carlsy S Ybanez, Anthony Ruquet, Paolo E Forni
  1. Department of Biological Sciences, The Center for Neuroscience Research, The RNA Institute, University at Albany, State University of New York, Albany, NY 12222, USA
Institutions: State University of New York (United States); University at Albany, State University of New York (United States)
Journal: Development (Cambridge, England), volume 153, issue 12, article dev205386
Dates: received 19 November 2025; accepted 21 May 2026; published online 22 June 2026; in print June 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1242/dev.205386 · PMID 42299035 · PMCID PMC13354959 · OpenAlex W4416387636
Open access: hybrid, a free copy (OpenAlex)
Preprint: doi:10.1101/2025.11.19.689260
Status: data only (how the code is found and verified)
Categories: mouse (organism), cellular / molecular (subfield)
Methods: Statistics, Smoothing, state filtering, decompositions, Connectivity, Machine learning
Keywords: Canopy 1 (Cnpy1), Vomeronasal neurons, Endoplasmic reticulum, ER stress, V2R receptors, Tfap2e (Ap-2ε)
MeSH: Endoplasmic Reticulum*, Receptors, Vasopressin*, Sensory Receptor Cells*, Vomeronasal Organ*, Animals, Mice, Mice, Knockout (* major topic)
Topic: Olfactory and Sensory Function Studies (Sensory Systems, Neuroscience), according to OpenAlex
Funding: National Institute on Deafness and Other Communication Disorders (R01DC017149); National Institute of Child Health and Human Development (R01HD09733, R01HD114827); NIH HHS (S10 OD028600); NIDCD NIH HHS (R01 DC017149, R01DC017149); Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD09733, R01HD114827); National Institutes of Health; NICHD NIH HHS (R01 HD114827, R01 HD097331)
Citations: not cited yet (Europe PMC); 64 references in the paper

Abstract

The vomeronasal organ (VNO) is a specialized chemosensory structure that detects chemical cues involved in predator avoidance, social interactions and reproductive behaviors. In mice, the VNO contains distinct vomeronasal sensory neurons (VSNs) that express vomeronasal receptors (VRs) and formyl peptide receptors. Apical VSNs express Meis2, V1Rs and Gαi2, whereas basal VSNs express Tfap2e (also known as AP-2ε), V2Rs and Gαo. Type 2 VRs (V2Rs) are classified into families A-E, and basal neurons co-express a family C receptor with a VR from another family. Single-cell RNA-sequencing identified ∼980 genes differentially expressed between V1R- and V2R-expressing neurons, many of which are linked to endoplasmic reticulum (ER) functions. Notably, canopy 1 (Cnpy1) is highly enriched in V2R-expressing neurons. The VNO of Cnpy1 knockout mice develops normally but undergoes progressive loss of V2R-expressing neurons, which is associated with increased ER stress gene expression, upregulation of family C V2R mRNAs and reduced V2R protein levels. V2R-expressing VSNs in Cnpy1 knockouts fail to respond to pheromones, show altered guidance and adhesion gene expression, and display disrupted connectivity with the accessory olfactory bulb. These findings emphasize the role of cell-specific ER protein repertoires in maintaining neuronal function.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper links to its data and to no code of its authors: see the Data section.

Tracing map

A tracing map links a paper to the code its authors published: this paper has none, so it has no map.

Data

Datasets cited

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 2, 28 September 2026

  • Publisher: n/a → The Company of Biologists

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 7 authors, 6 keywords, 7 MeSH terms, 7 funders, 64 references.

Cite

This paper

Mathias, N. A., Dolphin, N. M., LeFever, N. M., Amato, E., Ybanez, C. S., Ruquet, A., & Forni, P. E. (2026). Loss of the endoplasmic reticulum protein canopy 1 disrupts the function and circuit organization of V2R-expressing vomeronasal sensory neurons. Development (Cambridge, England), 153(12), dev205386. https://doi.org/10.1242/dev.205386

BibTeX

@article{mathias2026loss,
author = {Mathias, Nicholas A and Dolphin, Nikki M and LeFever, Noah M and Amato, Enrico and Ybanez, Carlsy S and Ruquet, Anthony and Forni, Paolo E},
title = {{Loss of the endoplasmic reticulum protein canopy 1 disrupts the function and circuit organization of V2R-expressing vomeronasal sensory neurons}},
journal = {Development (Cambridge, England)},
year = {2026},
month = jun,
volume = {153},
number = {12},
pages = {dev205386},
publisher = {Company of Biologists},
issn = {0950-1991},
doi = {10.1242/dev.205386},
url = {https://doi.org/10.1242/dev.205386},
pmid = {42299035},
pmcid = {PMC13354959}
}

RIS

TY - JOUR
AU - Mathias, Nicholas A
AU - Dolphin, Nikki M
AU - LeFever, Noah M
AU - Amato, Enrico
AU - Ybanez, Carlsy S
AU - Ruquet, Anthony
AU - Forni, Paolo E
TI - Loss of the endoplasmic reticulum protein canopy 1 disrupts the function and circuit organization of V2R-expressing vomeronasal sensory neurons
T2 - Development (Cambridge, England)
J2 - Development
PY - 2026
DA - 2026/06/19
VL - 153
IS - 12
SP - dev205386
SN - 0950-1991
PB - Company of Biologists
DO - 10.1242/dev.205386
UR - https://doi.org/10.1242/dev.205386
LA - en
ER -

CSL-JSON

{
"id": "10.1242/dev.205386",
"type": "article-journal",
"title": "Loss of the endoplasmic reticulum protein canopy 1 disrupts the function and circuit organization of V2R-expressing vomeronasal sensory neurons",
"container-title": "Development (Cambridge, England)",
"author": [
{
"family": "Mathias",
"given": "Nicholas A"
},
{
"family": "Dolphin",
"given": "Nikki M"
},
{
"family": "LeFever",
"given": "Noah M"
},
{
"family": "Amato",
"given": "Enrico"
},
{
"family": "Ybanez",
"given": "Carlsy S"
},
{
"family": "Ruquet",
"given": "Anthony"
},
{
"family": "Forni",
"given": "Paolo E"
}
],
"container-title-short": "Development",
"volume": "153",
"issue": "12",
"page": "dev205386",
"DOI": "10.1242/dev.205386",
"PMID": "42299035",
"PMCID": "PMC13354959",
"ISSN": "0950-1991",
"publisher": "Company of Biologists",
"URL": "https://doi.org/10.1242/dev.205386",
"language": "en",
"issued": {
"date-parts": [
[
2026,
6,
19
]
]
}
}

Similar papers

The papers with a page that share the most with this one: the tools found in their code, their categories, datasets, cited references and authors, the rarest counting most.

[1] doi:10.7554/elife.77259
Sociosexual behavior requires both activating and repressive roles of <i>Tfap2e/</i>AP-2ε in vomeronasal sensory neurons.
Journal: eLife
In common: mouse, cellular / molecular, 20 references, author Paolo E Forni
[2] doi:10.7554/elife.97356
Molecular, cellular, and developmental organization of the mouse vomeronasal organ at single cell resolution.
Journal: eLife
In common: mouse, cellular / molecular, 20 references
[3] doi:10.1073/pnas.2528466123
Cnpy1 is a candidate endoplasmic reticulum chaperone of vomeronasal type 2 GPCRs.
Journal: Proceedings of the National Academy of Sciences of the United States of America
In common: mouse, cellular / molecular, 16 references
[4] doi:10.1242/dev.200448
Notch signaling determines cell-fate specification of the two main types of vomeronasal neurons of rodents.
Journal: Development (Cambridge, England)
In common: mouse, cellular / molecular, 8 references, author Paolo E Forni
[5] doi:10.1002/glia.70057
Shared Lineage, Distinct Outcomes: Yap and Taz Loss Differentially Impact Schwann and Olfactory Ensheathing Cell Development Without Disrupting GnRH-1 Migration.
Journal: Glia
In common: mouse, cellular / molecular, 5 references, 2 authors
[6] doi:10.1242/jcs.263451
Stem cell expression of CXCR4 regulates tissue composition in the vomeronasal organ.
Journal: Journal of cell science
In common: mouse, cellular / molecular, 9 references
[7] doi:10.1002/dvg.70021
Tracing Early Migratory Neurons in the Developing Nose Using Contactin-2 (Cntn2) CreERT2.
Journal: Genesis (New York, N.Y. : 2000)
In common: mouse, 1 reference, 2 authors
[8] doi:10.1016/j.crmeth.2025.101216 [code]
Combinatorial responsiveness of chemosensory neurons in mouse explants revealed by DynamicNeuroTracker.
Journal: Cell reports methods
In common: mouse, 4 references
[9] doi:10.1126/sciadv.abn7450
Clustering of vomeronasal receptor genes is required for transcriptional stability but not for choice.
Journal: Science advances
In common: cellular / molecular, 3 references
[10] doi:10.1016/j.isci.2025.113078
Environmental stress promotes the persistence of juvenile traits in olfactory neurons as a protective mechanism.
Journal: iScience
In common: mouse, 3 references

Contribute

The authors of this paper can claim it, correct its record and validate its tracing map, and the maintainers of its code (its owner, or a public member of its organization) correct what it says of their repository; anyone signed in can ask for its removal. Every request goes to OSCR's own machine, which answers it; your account page follows them.

Sign in with ORCID to claim this paper as one of its authors, correct its record or validate its tracing map: when the paper's metadata lists your ORCID iD, you are recognized at once. Maintainers of its code: sign in with GitHub, then claim the repository on your account page.

Request its removal

To ask OSCR to remove this record, the copies of its authors' scripts or its tracing map, use the removal request page: signed in, you say who you are, what to remove and why, then review and confirm the request. Published rules decide every request (how).

Discussion, reproductions, activity

Discussion: code errors and mismatches between the code and the paper about this paper and its code, from readers and its authors, beside the paper and its tracing map.

Reproductions: reports from readers who ran the authors' code and did not get the paper's result, what they ran, with which environment, commit and data.

Activity: what happens around this paper: new versions of its record, its map's validation, discussions and reproductions. It opens with sign-in.