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Increased Glycine-N-methyltransferase expression disrupts light-dependent gene expression rhythms in the Drosophila eye.

Overview

Authors: Seth D. Lammert1, Makayla N. Marlin1, Danny M. Kanj1, Vikki M. Weake1,2,3
ORCID iDs: Seth D. Lammert, Makayla N. Marlin, Danny M. Kanj, Vikki M. Weake
  1. Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA
  2. Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA
  3. Purdue Institute for Integrative Neuroscience, Purdue University, West Lafayette, IN 47907, USA
Institutions: Purdue University West Lafayette (United States)
Journal: Journal of cell science, volume 139, issue 7, article jcs264529
Dates: received 27 October 2025; accepted 15 March 2026; published online 17 April 2026; in print April 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1242/jcs.264529 · PMID 41877655 · PMCID PMC13120682 · OpenAlex W7140279646
Open access: hybrid, a free copy (OpenAlex)
Status: data only
Categories: drosophila (organism), cellular / molecular (subfield)
Methods: Statistics, Evoked potentials
Keywords: Gnmt, Circadian rhythms, One-carbon metabolism, Methylation potential, Photoreceptor
MeSH: Circadian Rhythm*, Drosophila melanogaster*, Drosophila Proteins*, Eye*, Glycine N-Methyltransferase*, Light*, Animals, Gene Expression Regulation, Histones, Methylation, Photoreceptor Cells, Invertebrate (* major topic)
Topic: Epigenetics and DNA Methylation (Molecular Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: NIH (R01EY033734, R21AG094190); Purdue University
Citations: not cited yet (Europe PMC); 85 references in the paper
Research resources: anti-H3K9me3 RRID:AB_2797591, anti-IgG RRID:AB_2923178, anti-H3K4me1 RRID:AB_306847, anti-H3K27me3 RRID:AB_3665059, Japan) and anti-α-tubulin RRID:AB_579793

Abstract

Glycine-N-methyltransferase (Gnmt) is highly upregulated in the aging Drosophila melanogaster eye. Gnmt regulates S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) availability by catalyzing the transfer of a methyl group from SAM to glycine, producing sarcosine and SAH. Gnmt is well studied in the liver; however, little is known about the consequences of increased Gnmt in neurons. We overexpressed Gnmt in the eye and profiled diurnal rhythmic gene expression and histone methylation in photoreceptor neurons. Here, we show that eye-specific Gnmt overexpression altered rhythmic gene expression without impacting photoreceptor viability. Gnmt overexpression decreased global repressive histone methylation but had no major effect on H3K4 methylation, suggesting that methylation reactions are selectively inhibited by Gnmt and that Gnmt disrupts rhythmic gene expression independently of H3K4 methylation. Gnmt overexpression did not alter rhythmicity of core clock genes and did not impact circadian behavior. These results suggest that Gnmt plays a role in the regulation of light-dependent rhythmic gene expression in photoreceptors that does not involve the molecular clock.

Reproduced under the paper's license (CC BY), from the paper cited above.

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Version 1, 29 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 4 authors, 5 keywords, 11 MeSH terms, 2 funders, 85 references, 5 RRIDs.

Cite

This paper

Lammert, S. D., Marlin, M. N., Kanj, D. M., & Weake, V. M. (2026). Increased Glycine-N-methyltransferase expression disrupts light-dependent gene expression rhythms in the Drosophila eye. Journal of cell science, 139(7), jcs264529. https://doi.org/10.1242/jcs.264529

BibTeX

@article{lammert2026increased,
author = {Lammert, Seth D. and Marlin, Makayla N. and Kanj, Danny M. and Weake, Vikki M.},
title = {{Increased Glycine-N-methyltransferase expression disrupts light-dependent gene expression rhythms in the Drosophila eye}},
journal = {Journal of cell science},
year = {2026},
month = apr,
volume = {139},
number = {7},
pages = {jcs264529},
publisher = {The Company of Biologists},
issn = {0021-9533},
doi = {10.1242/jcs.264529},
url = {https://doi.org/10.1242/jcs.264529},
pmid = {41877655},
pmcid = {PMC13120682}
}

RIS

TY - JOUR
AU - Lammert, Seth D.
AU - Marlin, Makayla N.
AU - Kanj, Danny M.
AU - Weake, Vikki M.
TI - Increased Glycine-N-methyltransferase expression disrupts light-dependent gene expression rhythms in the Drosophila eye
T2 - Journal of cell science
J2 - J Cell Sci
PY - 2026
DA - 2026/04/17
VL - 139
IS - 7
SP - jcs264529
SN - 0021-9533
PB - The Company of Biologists
DO - 10.1242/jcs.264529
UR - https://doi.org/10.1242/jcs.264529
LA - en
ER -

CSL-JSON

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