Dynamic expectation strength and precision shape human pain perception through shared and dissociable α-oscillatory mechanisms.
Paper
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The authors' code
MATLAB · 82 lines · 3.1 KB · no license
- %% Mediation analysis basic walkthrough
- % This script is explained in more detail in the powerpoint titled
- % Mediation_sample_data_walkthrough
- % mediation_example_script1 and 2 do the same analysis.
- % ...script1 is very terse, and includes the essential commands only.
- % ...script2 is longer and includes more checking that files are available, etc.
- %% Step 1: Make a new analysis directory to save results, and go there
- % Make a new analysis directory to save results, and go there
- andir = 'Test_mediation';
- mkdir(andir)
- cd(andir)
- %% Step 2: Load image data and behavioral variables
- dinf = what('Wager_et_al_2008_Neuron_EmotionReg');
- %imgs = filenames(fullfile(dinf.path,'con_*img'), 'char', 'absolute');
- imgs = fullfile(dinf.path, 'Wager_2008_emo_reg_vs_look_neg_contrast_images.nii.gz');
- behav_dat = importdata(fullfile(dinf.path,'Wager_2008_emotionreg_behavioral_data.txt'))
- %% Step 3: Load and display mask
- % The mask determines which voxels are analyzed.
- % The standard mask is in the CanlabCore Tools repository, so you need the
- % folder containing it (and other CanlabCore folders) on your path.
- mask = which('gray_matter_mask.img')
- canlab_results_fmridisplay(mask, 'compact2');
- %% Step 4 : Run mediation
- % Run mediation without bootstrapping (fast)
- % Test that things are working
- x=behav_dat.data(:,1);
- y=behav_dat.data(:,2);
- names = {'X:RVLPFC' 'Y:Reappraisal_Success' 'M:BrainMediator'};
- % This is what you would run:
- % results = mediation_brain(x,y,imgs,'names',names,'mask', mask);
- % We run this instead to suppress output for report publishing
- str = 'results = mediation_brain(x,y,imgs,''names'',names,''mask'', mask);';
- disp(['Running with output suppressed (for report-generation): ' str]);
- evalc(str);
- % "Legacy" version: reslice mask to same space first:
- % scn_map_image(mask,deblank(imgs(1,:)), 'write', 'resliced_mask.img');
- % results = mediation_brain(x,y,imgs,'names',names,'mask', 'resliced_mask.img');
- % OR
- % ---------------------------------
- % Uncomment the lines below to run with bootstrapping
- % Make yourself a cup of tea while the results are compiled as this is going to take a while
- % pre-compiled results are also available in
- % 'mediation_Example_Data_Wager2008_Msearch_R_XisRIFGstim_norobust'
- % results = mediation_brain(x,y,imgs,'names',names,'mask', mask,'boot','pvals',5, 'bootsamples', 10000);
- %% Step 5: Get and save results figures, tables, and report
- % First, change to the mediation analysis directory, if you're not there already.
- % Then, run one of several batch results functions.
- %
- % The most complete way to create and publish a time- and date-stamped HTML
- % report with figures and tables is to run "publish_mediation_report.m"
- % (This is commented out because this script is published, and we can't
- % have nested publish commands)
- %
- % publish_mediation_report;
- %
- % ---------------------------------------------------------------------
- % Another option is to use this batch script to create figures and tables,
- % and save .mat files with results:
- mediation_brain_results_all_script;
- % This function runs a series of calls to mediation_brain_results.m, which
- % is also a stand-alone function. Type "help mediation_brain_results" for more options.
mediation_brain_single_level_walkthrough1.m at commit 46bdc01, no license · at the source
Overview
- School of Psychology, Shenzhen University, Shenzhen, China
- Department of Psychological and Brain Sciences, Dartmouth College, Hanover, New Hampshire, United States of America
- State Key Lab of Cognitive Science and Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, China
- Department of Psychology, University of Chinese Academy of Sciences, Beijing, China
Abstract
Human pain perception is not solely driven by sensory input but is dynamically modulated by what we expect to feel and how confident we are in those expectations. Yet, the temporal mechanisms through which evolving expectations shape pain remain poorly understood. Here, we combined a probabilistic cueing paradigm with computational modeling and EEG to dissociate two core components of expectation: strength (a recency-weighted estimate of predicted pain) and precision (the inverse variability of recent predictions). Trial-wise strength estimates closely tracked subjective expectations and outperformed static cue labels, validating the model’s psychological relevance. Expectation strength and precision exerted dissociable effects on pain processing: strength enhanced, whereas precision suppressed, pain-evoked responses. Critically, anticipatory α-band activity mediated these effects via distinct topographical patterns—expectation strength reduced fronto-central α power (reflecting heightened vigilance), while precision increased contralateral sensorimotor α-synchronization (supporting sensory gating). Source-level mediation analyses identified a right-lateralized dorsolateral prefrontal–sensorimotor cortices (DLPFC-SM1) integrating both components, with strength-specific engagement of the medial prefrontal cortex (mPFC). These effects were supported by Bayesian inference and pooled mega-analyses, underscoring their robustness. Together, these findings highlight cortical α-oscillations as dual-control mechanisms for predictive integration, with DLPFC–SM1 as a shared expectation hub and mPFC as a strength-specific node. By moving beyond static cue-based models, this framework captures the adaptive dynamics of expectation and provides a neurocomputational foundation for targeted interventions in chronic pain.
Reproduced under the paper's license (CC BY), from the paper cited above.
Repository
Its files are read in the Code ↔ Paper reader above.
canlab/MediationToolbox
46bdc01df8c3b22900a8cf89090fe8eea27ecc2d, 11 September 2026Availability: 1 check, the latest on 30 September 2026: the link answers
- 30 September 2026: the link answers
202 files
- Mediation_walkthrough/
bmrk3_download_and_prep_ , MATLAB, not shown heredata.mlx - Mediation_walkthrough/
mediation_1_basics.mlx , MATLAB, not shown here - Mediation_walkthrough/
mediation_brain_multilev , MATLAB, not shown hereel_walkthrough1.mlx - Mediation_walkthrough/
mediation_brain_single_l , MATLAB, not shown hereevel_walkthrough1.mlx - Mediation_walkthrough/
older/ , MATLAB, 82 linesmediation_brain_single_l evel_walkthrough1.m - Mediation_walkthrough/
older/ , MATLAB, 114 linesmediation_brain_single_l evel_walkthrough2.m - PDM_toolbox/
BootPDM.m , MATLAB, 150 lines - PDM_toolbox/
BootPDMJoint.m , MATLAB, 145 lines - PDM_toolbox/
Multivariate_Mediation_E , MATLAB, 111 linesxampleScript.m - PDM_toolbox/
PDMN.m , MATLAB, 193 lines - PDM_toolbox/
PVD.m , MATLAB, 264 lines - PDM_toolbox/
multivariateMediation.m , MATLAB, 494 lines - PDM_toolbox/
plotPDM.m , MATLAB, 41 lines - PDM_toolbox/
runBootstrapPDM.m , MATLAB, 96 lines - PDM_toolbox/
runPDM.m , MATLAB, 96 lines - PDM_toolbox/
thresholdPDM.m , MATLAB, 112 lines - geom2d/
Contents.m , MATLAB, 158 lines - geom2d/
angle3Points.m , MATLAB, 34 lines - geom2d/
angleSort.m , MATLAB, 62 lines - geom2d/
bisector.m , MATLAB, 59 lines - geom2d/
cart2geod.m , MATLAB, 43 lines - geom2d/
cartesianLine.m , MATLAB, 33 lines - geom2d/
centroid.m , MATLAB, 63 lines - geom2d/
circleArcAsCurve.m , MATLAB, 40 lines - geom2d/
circleAsPolygon.m , MATLAB, 33 lines - geom2d/
clipEdge.m , MATLAB, 109 lines - geom2d/
clipLineRect.m , MATLAB, 47 lines - geom2d/
clipPolygon.m , MATLAB, 63 lines - geom2d/
clipPolygonHP.m , MATLAB, 86 lines - geom2d/
convexification.m , MATLAB, 74 lines - geom2d/
crackPattern.m , MATLAB, 158 lines - geom2d/
crackPattern2.m , MATLAB, 119 lines - geom2d/
createCircle.m , MATLAB, 44 lines - geom2d/
createDirectedCircle.m , MATLAB, 49 lines - geom2d/
createEdge.m , MATLAB, 92 lines - geom2d/
createLine.m , MATLAB, 126 lines - geom2d/
createMedian.m , MATLAB, 50 lines - geom2d/
curvature.m , MATLAB, 152 lines - geom2d/
curveCentroid.m , MATLAB, 58 lines - geom2d/
curveLength.m , MATLAB, 56 lines - geom2d/
distancePointEdge.m , MATLAB, 64 lines - geom2d/
distancePointLine.m , MATLAB, 53 lines - geom2d/
distancePoints.m , MATLAB, 126 lines - geom2d/
drawArrow.m , MATLAB, 118 lines - geom2d/
drawCenteredEdge.m , MATLAB, 75 lines - geom2d/
drawCircle.m , MATLAB, 48 lines - geom2d/
drawCircleArc.m , MATLAB, 53 lines - geom2d/
drawCurve.m , MATLAB, 104 lines - geom2d/
drawEdge.m , MATLAB, 93 lines - geom2d/
drawEllipse.m , MATLAB, 91 lines - geom2d/
drawEllipseArc.m , MATLAB, 77 lines - geom2d/
drawLabels.m , MATLAB, 35 lines - geom2d/
drawLine.m , MATLAB, 54 lines - geom2d/
drawParabola.m , MATLAB, 57 lines - geom2d/
drawPoint.m , MATLAB, 74 lines - geom2d/
drawPolygon.m , MATLAB, 86 lines - geom2d/
drawRay.m , MATLAB, 74 lines - geom2d/
drawRect.m , MATLAB, 73 lines - geom2d/
drawRect2.m , MATLAB, 80 lines - geom2d/
drawShape.m , MATLAB, 78 lines - geom2d/
edgeAngle.m , MATLAB, 22 lines - geom2d/
edgeLength.m , MATLAB, 31 lines - geom2d/
ellipseAsPolygon.m , MATLAB, 49 lines - geom2d/
enclosingCircle.m , MATLAB, 66 lines - geom2d/
fillPolygon.m , MATLAB, 81 lines - geom2d/
geod2cart.m , MATLAB, 34 lines - geom2d/
hexagonalGrid.m , MATLAB, 88 lines - geom2d/
homothecy.m , MATLAB, 28 lines - geom2d/
inCircle.m , MATLAB, 23 lines - geom2d/
intersectEdges.m , MATLAB, 95 lines - geom2d/
intersectLineEdge.m , MATLAB, 78 lines - geom2d/
intersectLinePolygon.m , MATLAB, 43 lines - geom2d/
intersectLines.m , MATLAB, 80 lines - geom2d/
invertLine.m , MATLAB, 28 lines - geom2d/
isLeftOriented.m , MATLAB, 26 lines - geom2d/
lineAngle.m , MATLAB, 33 lines - geom2d/
lineFit.m , MATLAB, 96 lines - geom2d/
linePosition.m , MATLAB, 55 lines - geom2d/
lineSymmetry.m , MATLAB, 34 lines - geom2d/
medialAxisConvex.m , MATLAB, 126 lines - geom2d/
medianLine.m , MATLAB, 49 lines - geom2d/
minDistance.m , MATLAB, 38 lines - geom2d/
minDistancePoints.m , MATLAB, 167 lines - geom2d/
normalize.m , MATLAB, 28 lines - geom2d/
onCircle.m , MATLAB, 22 lines - geom2d/
onEdge.m , MATLAB, 52 lines - geom2d/
onLine.m , MATLAB, 56 lines - geom2d/
onRay.m , MATLAB, 42 lines - geom2d/
orthogonalLine.m , MATLAB, 31 lines - geom2d/
parallelLine.m , MATLAB, 34 lines - geom2d/
parametrize.m , MATLAB, 40 lines - geom2d/
pointOnLine.m , MATLAB, 21 lines - geom2d/
polarPoint.m , MATLAB, 52 lines - geom2d/
polyfit2.m , MATLAB, 183 lines - geom2d/
polygonArea.m , MATLAB, 53 lines - geom2d/
polygonCentroid.m , MATLAB, 41 lines - geom2d/
polygonClipHP.m , MATLAB, 18 lines - geom2d/
polygonExpand.m , MATLAB, 64 lines - geom2d/
polygonLength.m , MATLAB, 37 lines - geom2d/
polygonNormalAngle.m , MATLAB, 51 lines - geom2d/
projPointOnLine.m , MATLAB, 41 lines - geom2d/
readPolygon.m , MATLAB, 30 lines - geom2d/
rectAsPolygon.m , MATLAB, 48 lines - geom2d/
rotation.m , MATLAB, 57 lines - geom2d/
scaling.m , MATLAB, 45 lines - geom2d/
squareGrid.m , MATLAB, 50 lines - geom2d/
steinerPoint.m , MATLAB, 43 lines - geom2d/
steinerPolygon.m , MATLAB, 26 lines - geom2d/
supportFunction.m , MATLAB, 48 lines - geom2d/
surfaceCurvature.m , MATLAB, 28 lines - geom2d/
transformEdge.m , MATLAB, 41 lines - geom2d/
transformLine.m , MATLAB, 39 lines - geom2d/
transformPoint.m , MATLAB, 66 lines - geom2d/
translation.m , MATLAB, 38 lines - geom2d/
triangleGrid.m , MATLAB, 35 lines - geom2d/
vecnorm_geom2d_renamed.m , MATLAB, 57 lines - mediation_toolbox/
Boot_samples_needed_fcns , MATLAB, 79 lines/ Bneeded.m - mediation_toolbox/
Boot_samples_needed_fcns , MATLAB, 221 lines/ GLScalc_for_boot_L2M.m - mediation_toolbox/
Boot_samples_needed_fcns , MATLAB, 32 lines/ boot_rel_contrib_B.m - mediation_toolbox/
Boot_samples_needed_fcns , MATLAB, 13 lines/ v_from_alphaaccept.m - mediation_toolbox/
Boot_samples_needed_fcns , MATLAB, 18 lines/ v_from_targetu.m - mediation_toolbox/
Boot_samples_needed_fcns , MATLAB, 32 lines/ var2b.m - mediation_toolbox/
Boot_samples_needed_fcns , MATLAB, 111 lines/ var_prctile_script3.m - mediation_toolbox/
EM_step.m , MATLAB, 116 lines - mediation_toolbox/
HLM_EM_working.m , MATLAB, 138 lines - mediation_toolbox/
M3.m , MATLAB, 8 lines - mediation_toolbox/
RB_empirical_bayes_param , MATLAB, 87 liness_old.m - mediation_toolbox/
RB_hlm_working.m , MATLAB, 85 lines - mediation_toolbox/
ar_iterate_core.m , MATLAB, 92 lines - mediation_toolbox/
bootbca_ci.m , MATLAB, 97 lines - mediation_toolbox/
bootbca_pval.m , MATLAB, 136 lines - mediation_toolbox/
bootbca_pval_onetail.m , MATLAB, 177 lines - mediation_toolbox/
bootstrp_havesamples.m , MATLAB, 78 lines - mediation_toolbox/
dcm_sim.m , MATLAB, 106 lines - mediation_toolbox/
glmfit_multilevel_brain_ , MATLAB, 79 lineswrapper.m - mediation_toolbox/
mediation.m , MATLAB, 2,126 lines - mediation_toolbox/
mediationIVObserver.m , MATLAB, 78 lines - mediation_toolbox/
mediation_M_search.m , MATLAB, 21 lines - mediation_toolbox/
mediation_X_search.m , MATLAB, 15 lines - mediation_toolbox/
mediation_X_search_mask. , MATLAB, 8 linesm - mediation_toolbox/
mediation_Y_search.m , MATLAB, 12 lines - mediation_toolbox/
mediation_brain.m , MATLAB, 466 lines - mediation_toolbox/
mediation_brain_correcte , MATLAB, 164 linesd_threshold.m - mediation_toolbox/
mediation_brain_multilev , MATLAB, 121 lines_wrapper.m - mediation_toolbox/
mediation_brain_multilev , MATLAB, 293 linesel.m - mediation_toolbox/
mediation_brain_print_ta , MATLAB, 366 linesbles.m - mediation_toolbox/
mediation_brain_results. , MATLAB, 1,678 linesm - mediation_toolbox/
mediation_brain_results_ , MATLAB, 309 linesa_b_overlap.m - mediation_toolbox/
mediation_brain_results_ , MATLAB, 351 linesall_script.m - mediation_toolbox/
mediation_brain_results_ , MATLAB, 276 linesdetail.m - mediation_toolbox/
mediation_brain_results_ , MATLAB, 399 linesreport.m - mediation_toolbox/
mediation_brain_results_ , MATLAB, 69 linessurface_movie.m - mediation_toolbox/
mediation_brain_surface_ , MATLAB, 125 linesfigs.m - mediation_toolbox/
mediation_dcm_sim1.m , MATLAB, 488 lines - mediation_toolbox/
mediation_extract_data.m , MATLAB, 178 lines - mediation_toolbox/
mediation_latent.m , MATLAB, 218 lines - mediation_toolbox/
mediation_latent_sse.m , MATLAB, 129 lines - mediation_toolbox/
mediation_multilev_refor , MATLAB, 56 linesmat_cl.m - mediation_toolbox/
mediation_multilevel_loe , MATLAB, 49 linesss_plots.m - mediation_toolbox/
mediation_nmds_results.m , MATLAB, 304 lines - mediation_toolbox/
mediation_path_coefficie , MATLAB, 487 linesnts.m - mediation_toolbox/
mediation_path_coefficie , MATLAB, 222 linesnts_threepaths.m - mediation_toolbox/
mediation_path_coefficie , MATLAB, 330 linesnts_threepaths_singlelev el.m - mediation_toolbox/
mediation_path_diagram.m , MATLAB, 167 lines - mediation_toolbox/
mediation_permutation_sv , MATLAB, 193 linesc_fwe.m - mediation_toolbox/
mediation_plots.m , MATLAB, 375 lines - mediation_toolbox/
mediation_power.m , MATLAB, 307 lines - mediation_toolbox/
mediation_problem1.m , MATLAB, 58 lines - mediation_toolbox/
mediation_region_stepwis , MATLAB, 63 linese.m - mediation_toolbox/
mediation_results.m , MATLAB, 31 lines - mediation_toolbox/
mediation_results_XY_exc , MATLAB, 117 lineslusive_mask.m - mediation_toolbox/
mediation_results_intera , MATLAB, 175 linesctive_view_init.m - mediation_toolbox/
mediation_results_p2z.m , MATLAB, 31 lines - mediation_toolbox/
mediation_scatterplots.m , MATLAB, 222 lines - mediation_toolbox/
mediation_search.m , MATLAB, 176 lines - mediation_toolbox/
mediation_shift.m , MATLAB, 198 lines - mediation_toolbox/
mediation_shift_sse.m , MATLAB, 93 lines - mediation_toolbox/
mediation_sim1.m , MATLAB, 701 lines - mediation_toolbox/
mediation_sim2_igls.m , MATLAB, 471 lines - mediation_toolbox/
mediation_sim3.m , MATLAB, 246 lines - mediation_toolbox/
mediation_sim_output_fig , MATLAB, 143 liness.m - mediation_toolbox/
mediation_sim_single_lev , MATLAB, 402 linesel1.m - mediation_toolbox/
mediation_sim_single_lev , MATLAB, 205 linesel2.m - mediation_toolbox/
mediation_sort_xy_proxim , MATLAB, 743 linesity_plot.m - mediation_toolbox/
mediation_threepaths.m , MATLAB, 1,437 lines - mediation_toolbox/
mediation_threepaths_sim , MATLAB, 590 lines1.m - mediation_toolbox/
mediation_threepaths_sim , MATLAB, 130 lines_output_figs.m - mediation_toolbox/
mediation_threepaths_sin , MATLAB, 1,579 linesglelevel.m - mediation_toolbox/
moderation.m , MATLAB, 64 lines - mediation_toolbox/
multivariate_mediation_b , MATLAB, 375 linesrain_results_report.m - mediation_toolbox/
onsets2singletrial.m , MATLAB, 265 lines - mediation_toolbox/
onsets2singletrial_tor.m , MATLAB, 265 lines - mediation_toolbox/
optimal_delay_mediation_ , MATLAB, 26 linessearch.m - mediation_toolbox/
publish_mediation_report , MATLAB, 61 lines.m - mediation_toolbox/
publish_multivariate_med , MATLAB, 67 linesiation_report.m - mediation_toolbox/
setup_boot_samples.m , MATLAB, 60 lines - mediation_toolbox/
spm_config_mediation.m , MATLAB, 297 lines - mediation_toolbox/
timeseries_interactive_p , MATLAB, 109 lineslot.m - mediation_toolbox/
verification/ , MATLAB, 172 linesmediation_unit_test1.m - mediation_toolbox/
verification/ , MATLAB, 24 linesverify_bootstrap.m - mediation_toolbox/
xcorr_multisubject_old.m , MATLAB, 254 lines - README.md, Text, 147 lines
The paper's code and data availability statement is in the Data section.
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Proposed by the machine: these links were found in the paper and verified at the source, without human review. The map will receive a Zenodo DOI once one of the paper's authors has validated it with their ORCID.
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- 1 repository of the authors' code, each at its verified commit, with its license and how the link was found in the paper;
- 201 scripts, each with its path and the digest of its content;
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- neither the text of the paper nor the code itself.
Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.
Data
Datasets cited
- zenodo:18503056, at Zenodo; found in the text, “Validation of expectation manipulation”
Data Availability
All data needed to reproduce the conclusions and figures in the paper are present in the public repository of Zenodo (https://
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 1, 30 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 7 authors, 14 MeSH terms, 5 funders, 102 references.
Cite
This paper
Li, J., Chen, S., Zhang, L., Weng, L., Lin, X., Tu, Y., & Peng, W. (2026). Dynamic expectation strength and precision shape human pain perception through shared and dissociable α-oscillatory mechanisms. PLoS biology, 24(3), e3003675. https://
BibTeX
@article{li2026dynamic,
author = {Li, Jia and Chen, Shihao and Zhang, Libo and Weng, Lingling and Lin, Xinxin and Tu, Yiheng and Peng, Weiwei},
title = {{Dynamic expectation strength and precision shape human pain perception through shared and dissociable α-oscillatory mechanisms}},
journal = {PLoS biology},
year = {2026},
month = mar,
volume = {24},
number = {3},
pages = {e3003675},
publisher = {PLOS},
issn = {1544-9173},
doi = {10.1371/
url = {https://
pmid = {41770797},
pmcid = {PMC12965688}
}
RIS
TY - JOUR
AU - Li, Jia
AU - Chen, Shihao
AU - Zhang, Libo
AU - Weng, Lingling
AU - Lin, Xinxin
AU - Tu, Yiheng
AU - Peng, Weiwei
TI - Dynamic expectation strength and precision shape human pain perception through shared and dissociable α-oscillatory mechanisms
T2 - PLoS biology
J2 - PLoS Biol
PY - 2026
DA - 2026/
VL - 24
IS - 3
SP - e3003675
SN - 1544-9173
PB - PLOS
DO - 10.1371/
UR - https://
LA - en
ER -
CSL-JSON
{
"id": "10.1371/
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"title": "Dynamic expectation strength and precision shape human pain perception through shared and dissociable α-oscillatory mechanisms",
"container-title": "PLoS biology",
"author": [
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"family": "Li",
"given": "Jia"
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{
"family": "Zhang",
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{
"family": "Weng",
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{
"family": "Lin",
"given": "Xinxin"
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"family": "Tu",
"given": "Yiheng"
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"volume": "24",
"issue": "3",
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"URL": "https://
"language": "en",
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