Peroxisomal import is circadian in glia and regulates sleep and lipid metabolism.
The 1 match
- [1] § Results › Loss of Pex5 selectively in cortex glia disrupts sleep ↔ Sleep/DAM_2026_Das_metrics.R, lines 1088–1148 · score 0.57 · Sleep bout duration, beam crossings, Activity bouts, S1, min, day
Paper
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The authors' code
R · 3,769 lines · 129 KB · no license · 1 match
DAM_2026_Das_metrics.R at commit a60091d, no license · at the source
Overview
- Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, Iowa, United States of America
- Interdepartmental Neuroscience PhD Program, Iowa State University, Ames, Iowa, United States of America
- Department of Agriculture, Food, and Nutritional Science, University of Alberta, Edmonton, Canada
- Interdepartmental Genetics & Genomics PhD Program, Iowa State University, Ames, Iowa, United States of America
- Interdepartmental MCDB PhD Program, Iowa State University, Ames, Iowa, United States of America
- Department of Anatomy, University of California San Francisco, San Francisco, California, United States of America
Abstract
Peroxisomes are critical organelles that detoxify cellular waste while also catabolizing and anabolizing lipids. How peroxisomes coordinate protein import and support metabolic functions across complex tissues and timescales remains poorly understood in vivo. Using the Drosophila brain, we discover a striking enrichment of peroxisomes in the neuronal soma and the cortex glia that enwrap them. Unexpectedly, import of peroxisomal proteins into cortex glia, but not neurons, oscillated across time and peaked in the early morning. Rhythmic peroxisomal import in cortex glia autonomously required the circadian clock and Peroxin 5 (Pex5; peroxisomal biogenesis factor 5 homolog), with import persistently elevated in clock mutants. Notably, reducing Pex5 in cortex glia, but not neurons, caused hyperactivity and reduced total sleep. Moreover, brain lipid metabolism was dramatically altered upon Pex5 knockdown, with glia impacting sphingolipids and triacylglycerols, and neurons impacting phospholipids. The cell-type specificity of these Pex5 phenotypes highlights unique roles for peroxisomal import in both sleep and lipid metabolism in the brain.
Reproduced under the paper's license (CC BY), from the paper cited above.
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Zenodo 20637355
Availability: 1 check, the latest on 27 September 2026: the link answers (HTTP 200)
- 27 September 2026: the link answers (HTTP 200)
3 files
- Lipidomics/
2025_Das_reviews_metric. — R, 5,122 linesR - Sleep/
DAM_2026_Das_metrics.R — R, 3,769 lines - README.md — Text, 4 lines
jvaughen/das
a60091d58874746a4ea01594d3a18f26f69e6ddc, 10 June 2026Availability: 1 check, the latest on 27 September 2026: the link answers
- 27 September 2026: the link answers
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- Lipidomics/
2025_Das_reviews_metric. — R, 5,122 lines, shown from its sourceR - Sleep/
DAM_2026_Das_metrics.R — R, 3,769 lines, 1 match, shown from its source - README.md — Text, 4 lines, shown from its source
The paper's code and data availability statement is in the Data section.
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Data
No dataset and no data link were found in the paper.
Data Availability
Lipidomics raw data generated is available at the NIH Common Fund’s National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://
Reproduced under the paper's license (CC BY), from the paper cited above.
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Version 2, 28 September 2026
- Authors: added John P Vaughen (0000-0002-7141-1857); Hua Bai (0000-0003-2221-7545); removed John P Vaughen; Hua Bai
Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 11 authors, 13 MeSH terms, 9 funders, 108 references, 21 RRIDs.
Cite
This paper
Das, A., Rivas-Serna, I. M., Kumar, A., Sherpa, L., Huang, K., Kalita, H., Dorneich-Hayes, M., Liu, R., Mazurak, V. C., Vaughen, J. P., & Bai, H. (2026). Peroxisomal import is circadian in glia and regulates sleep and lipid metabolism. PLoS biology, 24(7), e3003901. https://
BibTeX
@article{das2026peroxiso
author = {Das, Anurag and Rivas-Serna, Irma Magaly and Kumar, Ankur and Sherpa, Lakpa and Huang, Kerui and Kalita, Hia and Dorneich-Hayes, Marlene and Liu, Ruiqi and Mazurak, Vera C and Vaughen, John P and Bai, Hua},
title = {{Peroxisomal import is circadian in glia and regulates sleep and lipid metabolism}},
journal = {PLoS biology},
year = {2026},
month = jul,
volume = {24},
number = {7},
pages = {e3003901},
publisher = {PLOS},
issn = {1544-9173},
doi = {10.1371/
url = {https://
pmid = {42455861},
pmcid = {PMC13387613}
}
RIS
TY - JOUR
AU - Das, Anurag
AU - Rivas-Serna, Irma Magaly
AU - Kumar, Ankur
AU - Sherpa, Lakpa
AU - Huang, Kerui
AU - Kalita, Hia
AU - Dorneich-Hayes, Marlene
AU - Liu, Ruiqi
AU - Mazurak, Vera C
AU - Vaughen, John P
AU - Bai, Hua
TI - Peroxisomal import is circadian in glia and regulates sleep and lipid metabolism
T2 - PLoS biology
J2 - PLoS Biol
PY - 2026
DA - 2026/
VL - 24
IS - 7
SP - e3003901
SN - 1544-9173
PB - PLOS
DO - 10.1371/
UR - https://
LA - en
ER -
CSL-JSON
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