Hierarchical Afferent Connectivity Drives Population-Wide Bursting Dynamics in a Computational Model of Human-Derived Excitatory Neuronal Networks.
The 19 matches
- [1] § Results ↔ Analysis/burst_detection.py, lines 17–119 · score 0.76 · bursting metrics, burst events, bursting rate, Firing rates, bursting activity, MBR
- [2] § Materials and Methods › Data analysis › Spiking, bursting, and network bursting analysis ↔ Analysis/burst_detection.py, lines 17–119 · score 0.72 · detected burst events, bursting rate, minute, interval, SpB, MBR
- [3] § Materials and Methods › Neuronal network model ↔ Model/Figures/generate_concurrent_topologies.py, lines 1–22 · score 0.70 · power law, outgoing connections, kept, concurrent, graph, Gaussian
- [4] § Materials and Methods › Neuronal network model ↔ Model/Main_network_model.ipynb, lines 21–137 · score 0.67 · dependent synaptic delays, synaptic connections, radius, um, distance, cells
- [5] § Materials and Methods › Neuronal network model ↔ Model/Figures/Suppl_Multiple_Networks_Idc_no_noise.ipynb, lines 20–156 · score 0.66 · AHP channel, Nernst potentials, voltage, potassium, sodium, adaptation
- [6] § Materials and Methods › Neuronal network model ↔ Model/Figures/Suppl_Multiple_Networks_Idc_no_noise.ipynb, lines 20–156 · score 0.66 · short term plasticity, NMDA conductance, ions, voltage, sums, synapses
- [7] § Materials and Methods › Neuronal network model ↔ Model/Figures/EPSP_EPSC_simulations.py, lines 14–27 · score 0.62 · membrane capacitance, Nernst potentials, noisy, potassium, sodium, Na
- [8] § Materials and Methods › Neuronal network model ↔ Model/Figures/Syn_STD.py, lines 72–84 · score 0.61 · available vesicles, short term depression, STD, synapse, presynaptic, modeled
- [9] § Materials and Methods › Data analysis › Principal component analysis ↔ Analysis/principal_component_analysis.ipynb, lines 11–56 · score 0.61 · principal component, transformation, variance, PCA, IFRs, weight
- [10] § Materials and Methods › Neuronal network model ↔ Model/Figures/EPSP_EPSC_simulations.py, lines 14–27 · score 0.60 · membrane capacitance, standard deviation, fluctuations, noisy, simulation, neuron
- [11] § Materials and Methods › Simulation protocol ↔ Model/Figures/Suppl_Integration_step_noise.ipynb, lines 22–130 · score 0.60 · numerical stability, exponential Euler, accuracy, simulated, dynamics, models
- [12] § Materials and Methods › Neuronal network model ↔ Model/Figures/Suppl_pacemakers.ipynb, lines 16–26 · score 0.59 · membrane capacitance, standard deviation, fluctuations, noisy, Neuronal, model
- [13] § Materials and Methods › Neuronal network model ↔ Model/Figures/delay_distribution.py, lines 21–37 · score 0.59 · Euclidean distance, SF networks, delay, SW, RND, connectivity
- [14] § Results ↔ Model/Figures/FI_curve_noise.py, lines 78–91 · score 0.59 · noise amplitudes, noise driven, Firing rates, curve, MFR, activity
- [15] § Materials and Methods › Neuronal network model ↔ Model/Main_network_model.ipynb, lines 21–137 · score 0.58 · membrane potential, NMDA conductance, ions, sums, synapses, channels
- [16] § Materials and Methods › Data analysis › Spiking, bursting, and network bursting analysis ↔ Analysis/fittingSTH.py, lines 55–57 · score 0.57 · double exponential, rising phase, curve, STH, fitting
- [17] § Results › Topological organization influences the temporal profile of population-wide burst dynamics ↔ Model/Figures/Suppl_Integration_step_noise.ipynb, lines 22–130 · score 0.56 · synaptic depression, synaptic conductance, noise, amplitude, temporal, intrinsic
- [18] § Results › Topological organization influences the temporal profile of population-wide burst dynamics ↔ Model/Figures/generate_concurrent_topologies.py, lines 1–22 · score 0.55 · concurrent topologies, outgoing connections, incoming
- [19] § Materials and Methods › Data analysis ↔ Model/Main_network_model.ipynb, lines 140–187 · score 0.54 · cumulative instantaneous firing, firing rates, durations, IFR, neuron
Paper
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The authors' code
Jupyter notebook · 187 lines · 7.1 KB · MIT · 3 matches
- # %% [markdown]
- # ## ================================================================
- # ## BRIAN2 SIMULATION OF A RECURRENT EXCITATORY NEURONAL NETWORK
- # ## ================================================================
- # This notebook simulates a population of excitatory neurons connected through **AMPA** and **NMDA** synapses using the *Brian2* simulator.
- # It includes intrinsic Hodgkin–Huxley-like neuron dynamics, synaptic transmission with short-term depression, and spatially dependent connection delays.
- # The simulation outputs **raster plots** and **population firing rates (cIFR)**.
- # %%
- # ------------------------------------------------
- # Import required packages
- # ------------------------------------------------
- !pip install brian2 -q
- from brian2 import *
- import numpy as np
- from time import time
- import matplotlib.pyplot as plt
- set_device('cpp_standalone', build_on_run=False)
- # %%
- # ================================================================
- # Parameter Setting
- # ================================================================
- # Define biophysical, synaptic, and network parameters.
- # These include Nernst potentials, conductances, time constants,
- # network geometry, and noise amplitude.
- Ne = 100 # Number of excitatory neurons
- El = -39.2 * mV # Nernst potential of leaky ions
- EK = -80 * mV # Nernst potential of potassium
- ENa = 70 * mV # Nernst potential of sodium
- VT = -30.4*mV # alters firing threshold of neurons
- E_ampa = 0*mV # reverse synaptic potential
- tau_ampa = 2*ms # synaptic time constant AMPA
- E_nmda = 0 * mV # Nernst potential of synaptic channels
- taud_nmda = 100 * ms # decay time constant of nmda conductance
- taur_nmda = 2 * ms # rise time constant of nmda conductance
- tau_d = 800 * ms # Recovery time constant of synaptic vesicles
- Vmax = 25*mm/second # Axonal conduction velocity
- radius = 160 * um # Network spatial radius
- fD = 0.0075 # Synaptic depression strength
- g_nmda = 0.0275*nS # NMDA synaptic conductance
- g_ampa = 0.35*nS # AMPA synaptic conductance
- sigma = 5.35*mV # Membrane potential oscillation amplitude
- # ================================================================
- # Definition of Cell Intrinsic and Synaptic Equations
- # ================================================================
- # Hodgkin–Huxley-like intrinsic dynamics with leak, Na+, and K+ currents.
- # Includes AMPA and NMDA synaptic currents and a stochastic noise term.
- eqs = '''
- dV/dt = (noise -gl*(V-El) - g_na*(m**3)*h*(V-ENa) - g_kd*(n**4)*(V-EK) - I_syn)/Cm : volt
- dm/dt = alpha_m*(1-m)-beta_m*m : 1
- dh/dt = alpha_h*(1-h)-beta_h*h : 1
- dn/dt = alpha_n*(1-n)-beta_n*n : 1
- alpha_m = 0.32*(mV**-1)*4*mV/exprel((13*mV-V+VT)/(4*mV))/ms : Hz
- beta_m = 0.28*(mV**-1)*5*mV/exprel((V-VT-40*mV)/(5*mV))/ms : Hz
- alpha_h = 0.128*exp((17*mV-V+VT)/(18*mV))/ms : Hz
- beta_h = 4./(1+exp((40*mV-V+VT)/(5*mV)))/ms : Hz
- alpha_n = 0.032*(mV**-1)*5*mV/exprel((15*mV-V+VT)/(5*mV))/ms : Hz
- beta_n = .5*exp((10*mV-V+VT)/(40*mV))/ms : Hz
- noise = sigma*(2*gl*Cm)**.5*randn()/sqrt(dt) : amp (constant over dt)
- I_syn = I_ampa+I_nmda : amp
- I_ampa = g_ampa*(V-E_ampa)*s_ampa : amp
- ds_ampa/dt = -s_ampa/tau_ampa : 1
- I_nmda = g_nmda*(V-E_nmda)*s_nmda_tot/(1+exp(-0.062*V/mV)/3.57) : amp
- s_nmda_tot : 1
- x : meter
- y : meter
- area : meter**2
- Cm = (1*uF*cm**-2) * area : farad
- g_na = (50*mS*cm**-2) * area : siemens
- g_kd = (5*mS*cm**-2) * area : siemens
- gl = (0.3*mS*cm**-2) * area : siemens
- '''
- eqs_synE_model = '''
- s_nmda_tot_post = s_nmda * x_d : 1 (summed)
- ds_nmda/dt = -s_nmda/(taud_nmda)+x_nmda*(1-s_nmda)/taur_nmda : 1 (clock-driven)
- dx_nmda/dt = -x_nmda/(taur_nmda) : 1 (clock-driven)
- dx_d/dt = (1-x_d)/tau_d :1 (clock-driven)
- '''
- eqs_synE_onpre = '''
- s_ampa += x_d
- x_nmda += 1
- x_d *= (1-fD)
- '''
- # ================================================================
- # Network Construction
- # ================================================================
- # Define neuron group, spatial layout, and synaptic connectivity.
- cellsExc = NeuronGroup(Ne, model=eqs, threshold='V>0*mV', refractory=2*ms, method='exponential_euler')
- cellsExc.area = 300*um**2
- cellsExc.V = El
- # Random spatial distribution within circular area
- cellsExc.x = np.sqrt(np.random.rand(Ne)) * radius * np.cos(2 * np.pi * np.random.rand(Ne))
- cellsExc.y = np.sqrt(np.random.rand(Ne)) * radius * np.sin(2 * np.pi * np.random.rand(Ne))
- # Load predefined structural connectivity (source/target pairs)
- sources = np.load('source_SF_RND_a2.npy') # to change accordingly
- targets = np.load('target_SF_RND_a2.npy') # to change accordingly
- # Create excitatory synapses
- syn_EE = Synapses(cellsExc, cellsExc, model=eqs_synE_model, on_pre=eqs_synE_onpre, method='euler')
- syn_EE.connect(i=sources, j=targets)
- # Distance-dependent synaptic delays
- syn_EE.delay = '(sqrt((x_pre - x_post)**2 + (y_pre - y_post)**2))/Vmax'
- # ================================================================
- # Simulation Execution and Spike Train saving
- # ================================================================
- # Run the simulation for a specified duration and record spike times.
- spikes = SpikeMonitor(cellsExc)
- dur = 65*second
- trans = 5*second
- run(dur)
- start_time = time()
- device.build(run=False)
- device.run()
- sp_t = spikes.t[spikes.t > trans]-trans
- sp_i = spikes.i[spikes.t > trans]
- np.save('spikesT.npy', sp_t/second)
- np.save('spikesI.npy', sp_i)
- print(f'Elapsed time: {(time()-start_time):.1f} seconds')
- # %%
- # ================================================================
- # Analysis and Visualization
- # ================================================================
- # Compute smoothed cumulative instantaneous firing rate (cIFR)
- # and generate raster and rate plots.
- def gaussian_window(window_size, sigma):
- """Return a normalized Gaussian kernel for smoothing."""
- x = np.linspace(-window_size // 2, window_size // 2, window_size)
- gauss = np.exp(-0.5 * (x / sigma) ** 2)
- return gauss / gauss.sum()
- # Parameters
- freqSam = 10_000 # Hz
- freqSam_sub = 1_000 # Hz
- acqTime = int((dur-trans)/second) # s
- n_sam = int(freqSam_sub * acqTime)
- smooth_window = 100 # ms
- smooth_window = int(smooth_window * freqSam_sub / 1000)
- # Cumulative IFR
- cum_spikes, sp_count = np.unique((sp_t/second*freqSam_sub).astype(int), return_counts=True)
- cum_peak = np.zeros(n_sam)
- cum_peak[cum_spikes] = sp_count
- cIFR = np.convolve(cum_peak, gaussian_window(smooth_window, smooth_window / 6) * freqSam_sub, mode='same')
- fig, axes = plt.subplots(2, 1, figsize=(12, 6), sharex=True,
- gridspec_kw={'height_ratios': [2, 1]})
- # Raster Plot
- axes[0].scatter(sp_t/second, sp_i, s=0.05, color='k')
- axes[0].set_xlim((0, acqTime))
- axes[0].set_ylim((-1, 100))
- axes[0].set_xticks([])
- axes[0].set_yticks([])
- axes[0].set_ylabel('Neuron index')
- # cIFR
- time_axis = np.arange(n_sam) / freqSam_sub
- axes[1].plot(time_axis, cIFR, color='skyblue')
- axes[1].set_xlim((0, acqTime))
- axes[1].set_xlabel('Time (s)')
- axes[1].set_ylabel('cIFR (sp/s)')
- fig.tight_layout()
- plt.show()
Main_network_model.ipynb at commit f61e17e, under MIT · at the source
Overview
- Neuroengineering Genoa Group, Department of Informatics, Bioengineering, Robotics and Systems Engineering (DIBRIS), University of Genova, Genova 16145, Italy
- IRCCS Ospedale Policlinico San Martino, Genova 16132, Italy
- National Institute for Nuclear Physics (INFN), Genova 16145, Italy
Abstract
This work presents a computational model of excitatory neuronal networks derived from human-induced pluripotent stem cells, whose activity was recorded with microelectrode arrays. A key feature of in vitro neuronal cultures is the emergence of network bursts (NBs)—population events involving most neurons, characterized by different durations, firing frequencies, and recruitment patterns. Our numerical approach investigates the mechanisms underlying these dynamics, addressing the limitations of experimental systems that make it difficult to isolate specific parameters and processes. The model aims to investigate how local neuronal dynamics and global structural connectivity interact to shape the emergence, propagation, and termination of NBs, highlighting the interdependence between intrinsic and network-level mechanisms. We demonstrate the critical role of noise in triggering NBs. At the same time, nonrandom, structured network topologies are essential for sustaining and shaping the resulting collective spatiotemporal firing patterns. In particular, we showed that the organization of incoming and outgoing degrees significantly modulates population recruitment and burst structure, with a hierarchical organization of afferent connectivity emerging as the dominant determinant of collective dynamics. By integrating in vitro observations into in silico simulations, the present study provides a solid foundation for understanding the principles governing human neuronal network function. Also, it sets the stage for investigating how alterations of network properties may contribute to pathological conditions.
Reproduced under the paper's license (CC BY), from the paper cited above.
Repositories
Its files are read in the Code ↔ Paper reader above, with 19 matches between paragraphs and lines of code.
Zenodo 15371493
Availability: 1 check, the latest on 30 September 2026: the link answers (HTTP 200)
- 30 September 2026: the link answers (HTTP 200)
21 files
- Analysis/
burst_detection.py , Python, 244 lines - Analysis/
fittingSTH.py , Python, 147 lines - Analysis/
principal_component_anal , Jupyter, 251 linesysis.ipynb - Analysis/
saveSTH.py , Python, 195 lines - Model/
Figures/ , Python, 168 linesEPSP_EPSC_simulations.py - Model/
Figures/ , Python, 91 linesFI_curve_noise.py - Model/
Figures/ , Python, 85 linesSuppl_FI_curve_Idc.py - Model/
Figures/ , Jupyter, 184 linesSuppl_Integration_step_I dc.ipynb - Model/
Figures/ , Jupyter, 210 linesSuppl_Integration_step_n oise.ipynb - Model/
Figures/ , Jupyter, 101 linesSuppl_Integration_step_p lot_error.ipynb - Model/
Figures/ , Jupyter, 161 linesSuppl_Multiple_Networks_ Idc.ipynb - Model/
Figures/ , Jupyter, 227 linesSuppl_Multiple_Networks_ Idc_no_noise.ipynb - Model/
Figures/ , Jupyter, 166 linesSuppl_Multiple_Networks_ gampa.ipynb - Model/
Figures/ , Jupyter, 197 linesSuppl_pacemakers.ipynb - Model/
Figures/ , Python, 150 linesSyn_STD.py - Model/
Figures/ , Python, 171 linesdegree_distribution.py - Model/
Figures/ , Python, 80 linesdelay_distribution.py - Model/
Figures/ , Python, 124 linesgenerate_concurrent_topo logies.py - Model/
Main_network_model.ipynb , Jupyter, 187 lines - LICENSE, License, 21 lines
- README.md, Text, 95 lines
screenneuropharm/humaninsilicomodel
f61e17e7b6f1b084c6b5199606d156698cf10988, 4 November 2025Availability: 1 check, the latest on 30 September 2026: the link answers
- 30 September 2026: the link answers
21 files
- Analysis/
burst_detection.py , Python, 244 lines, 2 matches - Analysis/
fittingSTH.py , Python, 147 lines, 1 match - Analysis/
principal_component_anal , Jupyter, 251 lines, 1 matchysis.ipynb - Analysis/
saveSTH.py , Python, 195 lines - Model/
Figures/ , Python, 168 lines, 2 matchesEPSP_EPSC_simulations.py - Model/
Figures/ , Python, 91 lines, 1 matchFI_curve_noise.py - Model/
Figures/ , Python, 85 linesSuppl_FI_curve_Idc.py - Model/
Figures/ , Jupyter, 184 linesSuppl_Integration_step_I dc.ipynb - Model/
Figures/ , Jupyter, 210 lines, 2 matchesSuppl_Integration_step_n oise.ipynb - Model/
Figures/ , Jupyter, 101 linesSuppl_Integration_step_p lot_error.ipynb - Model/
Figures/ , Jupyter, 161 linesSuppl_Multiple_Networks_ Idc.ipynb - Model/
Figures/ , Jupyter, 227 lines, 2 matchesSuppl_Multiple_Networks_ Idc_no_noise.ipynb - Model/
Figures/ , Jupyter, 166 linesSuppl_Multiple_Networks_ gampa.ipynb - Model/
Figures/ , Jupyter, 197 lines, 1 matchSuppl_pacemakers.ipynb - Model/
Figures/ , Python, 150 lines, 1 matchSyn_STD.py - Model/
Figures/ , Python, 171 linesdegree_distribution.py - Model/
Figures/ , Python, 80 lines, 1 matchdelay_distribution.py - Model/
Figures/ , Python, 124 lines, 2 matchesgenerate_concurrent_topo logies.py - Model/
Main_network_model.ipynb , Jupyter, 187 lines, 3 matches - LICENSE, License, 21 lines
- README.md, Text, 95 lines
Code accessibility
The network model files (Python) and the customized functions (Python) used to analyze both the simulated and experimental (reference) data have been deposited in Zenodo. The DOI of the code reported in this article is 10.5281/
Reproduced under the paper's license (CC BY), from the paper cited above.
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- 19 matches between paragraphs of the paper and lines of the code (method lexical-v1);
- neither the text of the paper nor the code itself.
Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.
Data
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Version 1, 30 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 4 authors, 6 keywords, 8 MeSH terms, 59 references.
Cite
This paper
Barabino, V., Callegari, F., Martinoia, S., & Massobrio, P. (2026). Hierarchical Afferent Connectivity Drives Population-Wide Bursting Dynamics in a Computational Model of Human-Derived Excitatory Neuronal Networks. The Journal of neuroscience : the official journal of the Society for Neuroscience, 46(17), e0912252026. https://
BibTeX
@article{barabino2026hie
author = {Barabino, Valerio and Callegari, Francesca and Martinoia, Sergio and Massobrio, Paolo},
title = {{Hierarchical Afferent Connectivity Drives Population-Wide Bursting Dynamics in a Computational Model of Human-Derived Excitatory Neuronal Networks}},
journal = {The Journal of neuroscience : the official journal of the Society for Neuroscience},
year = {2026},
month = apr,
volume = {46},
number = {17},
pages = {e0912252026},
publisher = {Society for Neuroscience},
issn = {0270-6474},
doi = {10.1523/
url = {https://
pmid = {41876230},
pmcid = {PMC13129645}
}
RIS
TY - JOUR
AU - Barabino, Valerio
AU - Callegari, Francesca
AU - Martinoia, Sergio
AU - Massobrio, Paolo
TI - Hierarchical Afferent Connectivity Drives Population-Wide Bursting Dynamics in a Computational Model of Human-Derived Excitatory Neuronal Networks
T2 - The Journal of neuroscience : the official journal of the Society for Neuroscience
J2 - J Neurosci
PY - 2026
DA - 2026/
VL - 46
IS - 17
SP - e0912252026
SN - 0270-6474
PB - Society for Neuroscience
DO - 10.1523/
UR - https://
LA - en
ER -
CSL-JSON
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