miR-133a-3p and miR-338-3p shape neural crest derivatives in zebrafish.
Overview
- Laboratorio de Biología Celular y Molecular del Desarrollo, Instituto de Biología Molecular y Celular de Rosario, CONICET-UNR, Rosario, Argentina
- Departamento de Zoología Genética y Antropología Física, Facultad de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain
- Laboratorio de Biotecnología Acuática, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina
Abstract
Neural crest cells are a transient, multipotent population that gives rise to diverse structures during vertebrate embryonic development, including craniofacial cartilage and pigment cells. Although the transcriptional regulation of neural crest development is well characterized, the role of microRNAs remains less understood. Using a double-transgenic zebrafish model expressing fluorescent reporters under the sox10 promoter, combined with fluorescence-activated cell sorting and RNA sequencing, we identified microRNAs enriched in neural crest cells. We focused on miR-133a-3p and miR-338-3p, previously linked to tumor suppression, to explore their developmental roles. The overexpression of either microRNA led to craniofacial cartilage malformations and reduced melanophore number, accompanied by decreased expression of key regulators including sox9b, sox10, and runx3. Reporter assays confirmed direct targeting of the sox9b 3′untranslated region. In addition, miR-338-3p overexpression increased neural crest cell numbers, suggesting a role in proliferation. These findings uncover novel functions for miR-133a-3p and miR-338-3p in vertebrate craniofacial and pigment cell development, highlighting shared regulatory features between embryogenesis and tumorigenesis.
Reproduced under the paper's license (CC BY), from the paper cited above.
Code
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Data
Datasets cited
- geo:GSE300363, at NCBI GEO; found in “Data Availability”
Other data links
- ncbi.nlm.nih.gov/
geo , NCBI; found in “Data Availability”
Data Availability
The miRNA-seq data from this publication have been deposited in the NCBI Gene Expression Omnibus (GEO) database (https://
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 1, 29 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 7 authors, 13 MeSH terms, 2 funders, 102 references.
Cite
This paper
Steeman, T. J., Torres, M., Weiner, A. M., Rubiolo, J. A., Sánchez, L. E., Coux, G., & Calcaterra, N. B. (2026). miR-133a-3p and miR-338-3p shape neural crest derivatives in zebrafish. Life science alliance, 9(7), e202503431. https://
BibTeX
@article{steeman2026mir,
author = {Steeman, Tomás J and Torres, Mercedes and Weiner, Andrea MJ and Rubiolo, Juan A and Sánchez, Laura E and Coux, Gabriela and Calcaterra, Nora B},
title = {{miR-133a-3p and miR-338-3p shape neural crest derivatives in zebrafish}},
journal = {Life science alliance},
year = {2026},
month = apr,
volume = {9},
number = {7},
pages = {e202503431},
publisher = {Life Science Alliance LLC},
issn = {2575-1077},
doi = {10.26508/
url = {https://
pmid = {41997877},
pmcid = {PMC13090132}
}
RIS
TY - JOUR
AU - Steeman, Tomás J
AU - Torres, Mercedes
AU - Weiner, Andrea MJ
AU - Rubiolo, Juan A
AU - Sánchez, Laura E
AU - Coux, Gabriela
AU - Calcaterra, Nora B
TI - miR-133a-3p and miR-338-3p shape neural crest derivatives in zebrafish
T2 - Life science alliance
J2 - Life Sci Alliance
PY - 2026
DA - 2026/
VL - 9
IS - 7
SP - e202503431
SN - 2575-1077
PB - Life Science Alliance LLC
DO - 10.26508/
UR - https://
LA - en
ER -
CSL-JSON
{
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"title": "miR-133a-3p and miR-338-3p shape neural crest derivatives in zebrafish",
"container-title": "Life science alliance",
"author": [
{
"family": "Steeman",
"given": "Tomás J"
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"family": "Torres",
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},
{
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},
{
"family": "Rubiolo",
"given": "Juan A"
},
{
"family": "Sánchez",
"given": "Laura E"
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{
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"given": "Gabriela"
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{
"family": "Calcaterra",
"given": "Nora B"
}
],
"container-title-short":
"volume": "9",
"issue": "7",
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"DOI": "10.26508/
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"ISSN": "2575-1077",
"publisher": "Life Science Alliance LLC",
"URL": "https://
"language": "en",
"issued": {
"date-parts": [
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