Brief mechanical pulses induce sustained intracellular L-lactate production in astrocytes.
Overview
- Laboratory of Neuroendocrinology-Molecular Cell Physiology, Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
- Celica Biomedical, Ljubljana, Slovenia
- Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
Abstract
Intracranial-pressure transients impose mechanical strain on perivascular astrocytes, but it is unclear whether brief mechanical events elicit metabolic responses. Here, we applied 1.5-s pressure-driven mechanical stimuli (5–1,000 hPa) to cultured primary rat cortical astrocytes using a patch-micropipette microinjector and monitored cytosolic lactate ([lactate]i) with a Förster resonance energy transfer Laconic nanosensor. Single pressure pulses (5–1,000 hPa) evoked a pressure-dependent increase in [lactate]i that persisted for minutes after stimulation, reaching a steady-state increase of 0.3–4.1% relative to baseline over 200–300 s. Sequential pulses delivered to the same cell produced transient spikes followed by stepwise increases in the post-stimulus plateau, yielding an estimated activation threshold of ~4–5 hPa. Transient receptor-potential vanilloid-4 (TRPV4) immunoreactivity was detected in cultured astrocytes, and pharmacologic inhibition of TRPV4 with HC-067047 attenuated mechanically evoked lactate accumulation during mechanical stimulation by solution bolus addition. These findings demonstrate that astrocytes convert brief mechanical stimuli into prolonged cytosolic lactate increases, supporting a contribution of TRPV4-associated mechanosensitive signaling to astrocytic mechano-metabolic integration.
Reproduced under the paper's license (CC BY), from the paper cited above.
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Data
Datasets cited
- zenodo:20737537, at Zenodo; found in DataCite
- zenodo:20737538, at Zenodo; found in DataCite
Data availability statement
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 2, 28 September 2026
- Funding: added European Commission
Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, pages, dates, 6 authors, 6 keywords, 67 references, 2 RRIDs.
Cite
This paper
Belko Parkel, K., Kuhanec, D., Gržina, Ž. T., Haque Chowdhury, H., Zorec, R., & Kreft, M. (2026). Brief mechanical pulses induce sustained intracellular L-lactate production in astrocytes. Frontiers in cellular neuroscience, 20, 1830831. https://
BibTeX
@article{belkoparkel2026
author = {Belko Parkel, Kaja and Kuhanec, Danaja and Gržina, Živa Tajda and Haque Chowdhury, Helena and Zorec, Robert and Kreft, Marko},
title = {{Brief mechanical pulses induce sustained intracellular L-lactate production in astrocytes}},
journal = {Frontiers in cellular neuroscience},
year = {2026},
month = jun,
volume = {20},
pages = {1830831},
publisher = {Frontiers Media SA},
issn = {1662-5102},
doi = {10.3389/
url = {https://
pmid = {42389485},
pmcid = {PMC13319024}
}
RIS
TY - JOUR
AU - Belko Parkel, Kaja
AU - Kuhanec, Danaja
AU - Gržina, Živa Tajda
AU - Haque Chowdhury, Helena
AU - Zorec, Robert
AU - Kreft, Marko
TI - Brief mechanical pulses induce sustained intracellular L-lactate production in astrocytes
T2 - Frontiers in cellular neuroscience
J2 - Front Cell Neurosci
PY - 2026
DA - 2026/
VL - 20
SP - 1830831
SN - 1662-5102
PB - Frontiers Media SA
DO - 10.3389/
UR - https://
LA - en
ER -
CSL-JSON
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"family": "Belko Parkel",
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{
"family": "Gržina",
"given": "Živa Tajda"
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"URL": "https://
"language": "en",
"issued": {
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