Increased Cannabinoid Receptor 1-Immunopositive Perisomatic Input of Principal Cells in Human Epileptic Patients in Focal Cortical Dysplasia Type IIB.

Overview

Authors: Cecília Szekeres-Paraczky^1,2, Péter Szocsics^1,2, Loránd Erőss³, Orsolya S Mihály⁴, Zsófia Maglóczky^1,2

Szentágothai János Doctoral School of Neuroscience, Semmelweis University, 1085 Budapest, Hungary; (C.S.-P.)
Human Brain Research Laboratory, Institute of Experimental Medicine, HUN-REN, 1083 Budapest, Hungary
Department of Functional Neurosurgery and Center of Neuromodulation, National Institute of Mental Health, Neurology and Neurosurgery, 1145 Budapest, Hungary
Department of Pathology, St. Borbála Hospital, 2800 Tatabánya, Hungary

Institutions: Semmelweis University (Hungary); HUN-REN Institute of Experimental Medicine (Hungary); Szent Borbála Kórház (Hungary)

Journal: International journal of molecular sciences, volume 27, issue 14, article 6326

Dates: received 25 May 2026; accepted 10 July 2026; published online 16 July 2026

Type: Research article · Language: English

License: CC BY

Identifiers: DOI 10.3390/ijms27146326 · PMID 42511668 · PMCID PMC13409960 · OpenAlex W7169609017

Open access: gold, a free copy (OpenAlex)

Status: code on request (how the code is found and verified)

Categories: histology / microscopy (modality), human (organism), epilepsy (population), cellular / molecular (subfield)

Methods: Statistics, fMRI & imaging

Keywords: perisomatic inhibition, dysmorphic neurons, human cortex, cannabinoid receptor 1, focal cortical dysplasia, interneurons

MeSH: Epilepsy*, Focal Cortical Dysplasia*, Malformations of Cortical Development, Group I*, Receptor, Cannabinoid, CB1*, Adolescent, Adult, Child, Child, Preschool, Female, Hippocampus, Humans, Male, Neurons, Parvalbumins, Young Adult (* major topic)

Topic: Neuroscience and Neuropharmacology Research (Cellular and Molecular Neuroscience, Neuroscience), according to OpenAlex

Funding: National Research, Development and Innovation Office (National Research, Development, and Innovation Office (NKFI, Hungary, grant number K 125436)

Citations: not cited yet (Europe PMC); 80 references in the paper

Abstract

Drug-resistant epilepsy is often associated with a neurodevelopmental disorder, focal cortical dysplasia (FCD). As demonstrated by numerous studies, defects in perisomatic inhibition may play a role in the formation of seizures. In our previous study, we observed an increase in parvalbumin-immunopositive perisomatic input. It has been observed that other perisomatic inhibitory cells expressing cannabinoid receptor type-1 (CB1R) and containing cholecystokinin (CCK) sprout in epileptic hippocampi. Therefore, we have investigated whether CB1R-immunopositive innervation has also changed in FCD. FCDIIB surgical samples were compared to controls with short post-mortem delay. The perisomatic input contacting principal cells was examined by NeuN-CB1R double immunostaining. Quantification in 3D was performed using a confocal fluorescence microscope. The results show that the CB1R-immunopositive synaptic input of principal cells is significantly larger in FCD cases. This condition may be an adaptive response to abnormal activity, or it may be a pre-existing pathology that is exacerbated by seizures. The reorganisation of the perisomatic inhibitory system has the potential to further increase the seizure probability in both cases. It should be noted that alterations in both PV- and CCK-CB1R-expressing basket cells may be implicated in seizure generation.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

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Data Availability Statement

All data and source code are available upon reasonable request from the corresponding authors. Some data are not publicly available due to ethical restrictions.

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Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 5 authors, 6 keywords, 15 MeSH terms, 1 funder, 80 references.

Cite

This paper

Szekeres-Paraczky, C., Szocsics, P., Erőss, L., Mihály, O. S., & Maglóczky, Z. (2026). Increased Cannabinoid Receptor 1-Immunopositive Perisomatic Input of Principal Cells in Human Epileptic Patients in Focal Cortical Dysplasia Type IIB. International journal of molecular sciences, 27(14), 6326. https://doi.org/10.3390/ijms27146326

BibTeX

@article{szekeresparaczky2026increased, author = {Szekeres-Paraczky, Cecília and Szocsics, Péter and Erőss, Loránd and Mihály, Orsolya S and Maglóczky, Zsófia}, title = {{Increased Cannabinoid Receptor 1-Immunopositive Perisomatic Input of Principal Cells in Human Epileptic Patients in Focal Cortical Dysplasia Type IIB}}, journal = {International journal of molecular sciences}, year = {2026}, month = jul, volume = {27}, number = {14}, pages = {6326}, publisher = {Multidisciplinary Digital Publishing Institute (MDPI)}, issn = {1422-0067}, doi = {10.3390/ijms27146326}, url = {https://doi.org/10.3390/ijms27146326}, pmid = {42511668}, pmcid = {PMC13409960} }

RIS

TY - JOUR AU - Szekeres-Paraczky, Cecília AU - Szocsics, Péter AU - Erőss, Loránd AU - Mihály, Orsolya S AU - Maglóczky, Zsófia TI - Increased Cannabinoid Receptor 1-Immunopositive Perisomatic Input of Principal Cells in Human Epileptic Patients in Focal Cortical Dysplasia Type IIB T2 - International journal of molecular sciences J2 - Int J Mol Sci PY - 2026 DA - 2026/07/16 VL - 27 IS - 14 SP - 6326 SN - 1422-0067 PB - Multidisciplinary Digital Publishing Institute (MDPI) DO - 10.3390/ijms27146326 UR - https://doi.org/10.3390/ijms27146326 LA - en ER -

CSL-JSON

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