POFUT1 Serves as an Independent Prognostic Factor and Therapeutic Target by Activating the PI3K/AKT Pathway in Glioma.
Overview
- Department of Neurosurgery, Shanxi Provincial People's Hospital, Shanxi Medical University, Taiyuan, China
- The Neurosurgery Department, Xi'an Xidian Hospital, Xi'an, China
Abstract
Objective: Protein O‐fucosyltransferase 1 (POFUT1) has been implicated in several malignancies, but its functional and prognostic significance in glioma remains insufficiently defined. This study evaluated whether POFUT1 expression is associated with glioma progression, patient outcome, and PI3K/
Methods: Public glioma transcriptome datasets from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) were analyzed and compared with clinical samples collected from 123 glioma patients. POFUT1 protein levels in clinical specimens were determined by immunohistochemical staining, and its association with patient outcome was analyzed using survival curves. In vitro, glioma cell growth, motility, and invasiveness were examined using MTT and Transwell assays. The effect of POFUT1 on tumor formation was further tested in a subcutaneous xenograft model. RNA sequencing, KEGG pathway enrichment, and pharmacological inhibition were then used to explore the mechanism linking POFUT1 to PI3K/
Results: POFUT1 expression was higher in glioma than in normal brain tissue and increased with tumor grade. Patients with high POFUT1 levels had shorter overall survival, and multivariate Cox analyses supported POFUT1 as an independent prognostic indicator. Incorporating POFUT1 into a nomogram improved prediction of 1‐, 3‐, and 5‐year survival. Functionally, POFUT1 knockdown reduced glioma cell growth, motility, invasion, and xenograft expansion, whereas POFUT1 overexpression produced the opposite phenotype. Transcriptomic and protein analyses indicated that POFUT1 enhanced PI3K/
Conclusion: POFUT1 as a key driver of glioma malignancy, predominantly through activating the PI3K‐AKT signaling pathway. These findings highlight POFUT1 as a promising novel therapeutic target for aggressive glioma.
Reproduced under the paper's license (CC BY), from the paper cited above.
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Data
Datasets cited
- geo:GSE316535 — at NCBI GEO; found in the text, “RNA Sequencing”
Data Availability Statement
The transcriptomic sequencing data obtained from this study have been archived in the GEO repository and can be accessed using the accession ID GSE316535 (https://
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 9 authors, 4 keywords, 15 MeSH terms, 3 funders, 24 references.
Cite
This paper
Yang, K., Ju, C., Wang, Y., Guo, A., Yang, X., Mi, Y., Su, Y., Wang, C., & Ji, H. (2026). POFUT1 Serves as an Independent Prognostic Factor and Therapeutic Target by Activating the PI3K/
BibTeX
@article{yang2026pofut1,
author = {Yang, Kai and Ju, Chunlei and Wang, Yifan and Guo, Aijie and Yang, Xiaochen and Mi, Yang and Su, Yifei and Wang, Chunhong and Ji, Hongming},
title = {{POFUT1 Serves as an Independent Prognostic Factor and Therapeutic Target by Activating the PI3K/
journal = {Brain and behavior},
year = {2026},
month = sep,
volume = {16},
number = {9},
pages = {e71701},
publisher = {Wiley},
issn = {2162-3279},
doi = {10.1002/
url = {https://
pmid = {42675310},
pmcid = {PMC13529811}
}
RIS
TY - JOUR
AU - Yang, Kai
AU - Ju, Chunlei
AU - Wang, Yifan
AU - Guo, Aijie
AU - Yang, Xiaochen
AU - Mi, Yang
AU - Su, Yifei
AU - Wang, Chunhong
AU - Ji, Hongming
TI - POFUT1 Serves as an Independent Prognostic Factor and Therapeutic Target by Activating the PI3K/
T2 - Brain and behavior
J2 - Brain Behav
PY - 2026
DA - 2026/
VL - 16
IS - 9
SP - e71701
SN - 2162-3279
PB - Wiley
DO - 10.1002/
UR - https://
LA - en
ER -
CSL-JSON
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