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Subgroup-Specific Associations of GRIA Genes Encoding AMPA Glutamate Receptor Subunits with Patient Survival in Medulloblastoma.

Overview

Authors: Bruno Saciloto1, Matheus Dalmolin2,3,4, Julia Caroline Marcolin4,5,6, Martina Lichtenfels4,7, Julia Vanini4,5, Isabella B. Schröder Roesler8, Jurandir M. Ribas Filho1, Osvaldo Malafaia1, Marcelo A.C. Fernandes2,3,4,9, Caroline Brunetto de Farias4,5,6,7, Amanda Thomaz4,10, Rafael Roesler4,5,6,11,12, Gustavo R. Isolan1,4,13
ORCID iDs: Amanda Thomaz
13 affiliations
  1. Graduate Program in Principles of Surgery, Mackenzie Evangelical University, Curitiba, 80730-000 Brazil
  2. InovAI Lab, nPITI/IMD, Federal University of Rio Grande do Norte,Natal, 59078-970 Brazil
  3. Bioinformatics Multidisciplinary Environment (BioME), Federal University of Rio Grande do Norte,Natal, 59078-970 Brazil
  4. National Science and Technology Institute for Children’s Cancer Biology and Pediatric Oncology—INCT BioOncoPed, Porto Alegre, 90035-003 Brazil
  5. Cancer and Neurobiology Laboratory, Experimental Research Center, Clinical Hospital (CPE-HCPA), Federal University of Rio Grande do Sul,Porto Alegre, 90035-903 Brazil
  6. Graduate Program in Biological Sciences: Pharmacology and Therapeutics, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul,Porto Alegre, 90035-003 Brazil
  7. Department of Translational Research, Ziel Biosciences, Porto Alegre, 90050-170 Brazil
  8. School of Medicine, Pontifical Catholic University of Rio Grande do Sul,Porto Alegre, 90610-000 Brazil
  9. Department of Computer Engineering and Automation, Federal University of Rio Grande do Norte,Natal, 59078-970 Brazil
  10. Department of Biology, Edge Hill University,Ormskirk, L39 4QP UK
  11. Department of Pharmacology, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul,Porto Alegre, 90035-003 Brazil
  12. Center for Biotechnology, Federal University of Rio Grande do Sul,Porto Alegre, 91501- 970 Brazil
  13. The Center for Advanced Neurology and Neurosurgery (CEANNE), Porto Alegre, 90560-010 Brazil
Institutions: Universidade Federal do Rio Grande do Norte (Brazil); Universidade Federal do Rio Grande do Sul (Brazil); Pontifícia Universidade Católica do Rio Grande do Sul (Brazil); Edge Hill University (United Kingdom)
Journal: Neuromolecular medicine, volume 28, issue 1, article 36
Dates: received 24 February 2026; accepted 29 May 2026; published online 11 June 2026; in print 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1007/s12017-026-08935-8 · PMID 42277445 · PMCID PMC13260187 · OpenAlex W7164387778
Open access: hybrid, a free copy (OpenAlex)
Status: data only
Categories: genetics / omics (modality), human (organism), other condition (population), cellular / molecular (subfield)
Methods: Statistics, Smoothing, state filtering, decompositions
Keywords: AMPA receptor, Glutamate receptor, GRIA, Medulloblastoma, Pediatric brain tumor, Brain cancer
MeSH: Brain Neoplasms*, Gene Expression Regulation, Neoplastic*, Medulloblastoma*, Receptors, AMPA*, Cell Line, Tumor, Datasets as Topic, Gene Expression Profiling, Humans, Organotechnetium Compounds, Oximes, Protein Subunits, RNA, Messenger, Single-Cell Gene Expression Analysis (* major topic)
Topic: Glioma Diagnosis and Treatment (Genetics, Medicine), according to OpenAlex
Funding: Universidade Federal Do Rio Grande Do Sul
Citations: cited by 2 papers (Europe PMC); 51 references in the paper

Abstract

Brain cancers hijack biological systems involved in neural development and synaptic plasticity. Medulloblastoma (MB), the most common malignant brain tumor in children, is thought to arise from disruptions in neurodevelopmental programs. Glutamatergic transmission mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors (AMPARs) has been implicated in synaptic communication between adult brain tumors and surrounding neurons; however, the possible role of AMPARs in MB remains largely unexplored. Here, we analyzed the expression of genes encoding AMPAR subunits, GRIA1–4, in datasets of MB tumors and cell lines, revealing distinct expression patterns and associations with overall survival (OS) across molecular subgroups and histological variants. Expression levels differed among MB molecular subgroups. Analysis using single-cell RNA sequencing (scRNA-seq) was consistent with enrichment of GRIA1 in Group 3 and GRIA4 in sonic hedgehog (SHH) MB. Higher GRIA1, GRIA2, and GRIA4 transcription was associated with more favorable patient outcomes in specific MB subgroups. In contrast, high expression of GRIA3 in SHH, or of either GRIA3 or GRIA4 in Group 3 MB, was associated with worse prognosis. Particularly robust but opposing associations with patient survival were found for GRIA3 and GRIA4 in SHH MB. Analysis of GRIA mRNA levels in MB cell lines using both quantitative reverse transcription polymerase chain reaction (qRT-PCR) and data from The Human Protein Atlas, supported some of the gene expression patterns observed in tumors. Together, these findings suggest that GRIA genes and their corresponding AMPAR subunits may have subgroup-specific prognostic relevance in MB.

Supplementary Information: The online version contains supplementary material available at 10.1007/s12017-026-08935-8.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper links to its data, not to its authors' code: see the Data section.

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Data

Data links

Data Availability

The tumor datasets used in this study are available in the Gene Expression Omnibus (GEO) repository, accession number GSE202043, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi? acc=GSE202043; accession number GSE85217, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi? acc=GSE85217, and GSE156053 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi? acc=GSE156053. The MB scRNA-seq single cell dataset is available at the Pediatric Neuro-oncology Cell Atlas (https://www.pneuroonccellatlas.org/). Data from cell lines were obtained from The Human Protein Atlas (https://www.proteinatlas.org/, accessed on January 27th 2026) and are available at https://www.proteinatlas.org/ENSG00000155511-GRIA1/cell+line#brain_cancer; https://www.proteinatlas.org/ENSG00000120251-GRIA2/cell+line#brain_cancer; https://www.proteinatlas.org/ENSG00000125675-GRIA3/cell+line#brain_cancer; https://www.proteinatlas.org/ENSG00000152578-GRIA4/cell+line#brain_cancer; https://www.proteinatlas.org/ENSG00000155511-GRIA1/cell+line#non-cancerous; https://www.proteinatlas.org/ENSG00000120251-GRIA2/cell+line#non-cancerous; https://www.proteinatlas.org/ENSG00000125675-GRIA3/cell+line#non-cancerous; and https://www.proteinatlas.org/ENSG00000152578-GRIA4/cell+line#non-cancerous.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 2, 28 September 2026

  • Publisher: n/a → Springer Science+Business Media

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 13 authors, 6 keywords, 13 MeSH terms, 1 funder, 50 references.

Cite

This paper

Saciloto, B., Dalmolin, M., Marcolin, J. C., Lichtenfels, M., Vanini, J., Roesler, I. B. S., Ribas Filho, J. M., Malafaia, O., Fernandes, M. A., de Farias, C. B., Thomaz, A., Roesler, R., & Isolan, G. R. (2026). Subgroup-Specific Associations of GRIA Genes Encoding AMPA Glutamate Receptor Subunits with Patient Survival in Medulloblastoma. Neuromolecular medicine, 28(1), 36. https://doi.org/10.1007/s12017-026-08935-8

BibTeX

@article{saciloto2026subgroup,
author = {Saciloto, Bruno and Dalmolin, Matheus and Marcolin, Julia Caroline and Lichtenfels, Martina and Vanini, Julia and Roesler, Isabella B. Schröder and Ribas Filho, Jurandir M. and Malafaia, Osvaldo and Fernandes, Marcelo A.C. and de Farias, Caroline Brunetto and Thomaz, Amanda and Roesler, Rafael and Isolan, Gustavo R.},
title = {{Subgroup-Specific Associations of GRIA Genes Encoding AMPA Glutamate Receptor Subunits with Patient Survival in Medulloblastoma}},
journal = {Neuromolecular medicine},
year = {2026},
month = jun,
volume = {28},
number = {1},
pages = {36},
publisher = {Springer Science+Business Media},
issn = {1535-1084},
doi = {10.1007/s12017-026-08935-8},
url = {https://doi.org/10.1007/s12017-026-08935-8},
pmid = {42277445},
pmcid = {PMC13260187}
}

RIS

TY - JOUR
AU - Saciloto, Bruno
AU - Dalmolin, Matheus
AU - Marcolin, Julia Caroline
AU - Lichtenfels, Martina
AU - Vanini, Julia
AU - Roesler, Isabella B. Schröder
AU - Ribas Filho, Jurandir M.
AU - Malafaia, Osvaldo
AU - Fernandes, Marcelo A.C.
AU - de Farias, Caroline Brunetto
AU - Thomaz, Amanda
AU - Roesler, Rafael
AU - Isolan, Gustavo R.
TI - Subgroup-Specific Associations of GRIA Genes Encoding AMPA Glutamate Receptor Subunits with Patient Survival in Medulloblastoma
T2 - Neuromolecular medicine
J2 - Neuromolecular Med
PY - 2026
DA - 2026/06/11
VL - 28
IS - 1
SP - 36
SN - 1535-1084
PB - Springer Science+Business Media
DO - 10.1007/s12017-026-08935-8
UR - https://doi.org/10.1007/s12017-026-08935-8
LA - en
ER -

CSL-JSON

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