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CBLN2 promoter enables genetic access to wide-field neurons of the tree shrew superior colliculus.

Overview

Authors: Arda Kipcak1, Alev Erisir1
ORCID iDs: Arda Kipcak, Alev Erisir
  1. Department of Psychology, University of Virginia, Charlottesville, VA 22903, USA
Institutions: University of Virginia (United States)
Journal: Cell reports methods, volume 6, issue 3, article 101309
Dates: received 7 August 2025; accepted 9 January 2026; published online 6 March 2026; in print March 2026
Type: Brief report · Language: English
License: CC BY
Identifiers: DOI 10.1016/j.crmeth.2026.101309 · PMID 41794023 · PMCID PMC13030960 · OpenAlex W7134137301
Open access: gold, a free copy (OpenAlex)
Status: code on request
Categories: other (organism), cellular / molecular (subfield)
Methods: Statistics, Evoked potentials, fMRI & imaging
Keywords: wide-field, CBLN2, superior colliculus, tectopulvinar, tree shrew, AAV
MeSH: Nerve Tissue Proteins*, Neurons*, Promoter Regions, Genetic*, Superior Colliculi*, Tupaiidae*, Animals, Dependovirus (* major topic)
Topic: Hearing, Cochlea, Tinnitus, Genetics (Sensory Systems, Neuroscience), according to OpenAlex
Funding: National Institute of Neurological Disorders and Stroke (U01NS122040); National Institutes of Health
Citations: cited by 1 paper (Europe PMC); 118 references in the paper
Research resources: Donkey Anti-Mouse AF488 (1:250) RRID:AB_141607, Donkey Anti-Goat Cy3 RRID:AB_2340411, Donkey Anti-Guinea Pig Cy5 (1:250) RRID:AB_2340462, Donkey Anti-Rabbit Cy5 (1:250) RRID:AB_2340607, Donkey Anti-Mouse TRITC (1:250) RRID:AB_2340767, Donkey Anti-Rabbit AF488 (1:250) RRID:AB_2535792, Rabbit Anti-CBLN2 Antibody (1:500) RRID:AB_2850949, M. musculus Mouse RRID:IMSR_JAX:000664

Abstract

Wide-field (WF) neurons of the tectopulvinar pathway integrate retinal and cortical inputs via large dendritic arbors crucial for rapid visual motion detection. Previous studies identified potential marker genes for mouse WF neurons. Here, we validate CBLN2 as a molecular marker of the tree shrew WF neurons and construct AAVs that exploit CBLN2 promoter to selectively target WF neurons across species. Using intersectional genetics in the tree shrew, we show that WF neuron dendrites receive a distinct pattern of VGluT1+ and VGluT2+ inputs based on their distance from the cell body in the dorsoventral axis of the superior colliculus (SC). This represents the first example of a viral tool derived from the tree shrew genome for cell-type-specific targeting across species. Our results provide a foundation for studying SC circuitry in higher-order mammals and for extending this approach to additional conserved cell types in the SC and other brain regions.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper says that its authors' code is available on request: it was not published with the paper, so there is nothing to verify.

The paper's code and data availability statement is in the Data section.

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Data

Datasets cited

Data and code availability

All genetic data are available in this paper’s supplemental information. Existing, publicly available genomic and epigenomic data used in this paper are accessible at respective repositories reported in the key resources table.

All original code is deposited to UVA DataVerse and is publicly available at https://doi.org/10.18130/V3/NEGJWP as of the publication date.

Any additional information regarding the data and analysis reported in this paper is available from the lead contact upon request.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

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Version 1, 30 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 2 authors, 6 keywords, 7 MeSH terms, 2 funders, 109 references, 8 RRIDs.

Cite

This paper

Kipcak, A., & Erisir, A. (2026). CBLN2 promoter enables genetic access to wide-field neurons of the tree shrew superior colliculus. Cell reports methods, 6(3), 101309. https://doi.org/10.1016/j.crmeth.2026.101309

BibTeX

@article{kipcak2026cbln2,
author = {Kipcak, Arda and Erisir, Alev},
title = {{CBLN2 promoter enables genetic access to wide-field neurons of the tree shrew superior colliculus}},
journal = {Cell reports methods},
year = {2026},
month = mar,
volume = {6},
number = {3},
pages = {101309},
publisher = {Elsevier},
issn = {2667-2375},
doi = {10.1016/j.crmeth.2026.101309},
url = {https://doi.org/10.1016/j.crmeth.2026.101309},
pmid = {41794023},
pmcid = {PMC13030960}
}

RIS

TY - JOUR
AU - Kipcak, Arda
AU - Erisir, Alev
TI - CBLN2 promoter enables genetic access to wide-field neurons of the tree shrew superior colliculus
T2 - Cell reports methods
J2 - Cell Rep Methods
PY - 2026
DA - 2026/03/06
VL - 6
IS - 3
SP - 101309
SN - 2667-2375
PB - Elsevier
DO - 10.1016/j.crmeth.2026.101309
UR - https://doi.org/10.1016/j.crmeth.2026.101309
LA - en
ER -

CSL-JSON

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