Epigenomic regulation of neural crest differentiation in human-induced pluripotent stem cells.
Overview
- Research Institute for Clinical Oncology, Saitama Cancer Center, Saitama, Japan
- Department of Graduate School of Science and Engineering, Saitama University, Saitama, Japan
- Department of Life Engineering, Faculty of Engineering, Maebashi Institute of Technology, Maebashi, Gunma, Japan
- Department of Epigenomics, Institute for Advanced Life Sciences, Hoshi University, Tokyo, Japan
- Laboratory of Integrative Metabolic Regulation, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan
Abstract
Neural crest cells (NCCs) drive vertebrate development through lineage specific differentiation into diverse tissues. Cranial (cNCCs) and trunk NCCs (tNCCs) exhibit distinct developmental trajectories that require coordinated epigenomic regulation. Aberrant NCC differentiation is associated with diseases including tumors. Epigenetic mechanisms such as histone modifications and DNA methylation are crucial for regulating NCC differentiation. Here, we used a human induced pluripotent stem cell model to compare differentiation of cNCCs and tNCCs and define lineage specific mechanisms. Integrated transcriptome and DNA methylome analyses revealed that DNA demethylation upstream of MEF2C in cNCCs and the THRA locus (a shared promoter region for THRA1 and THRA2 isoforms) in tNCCs was associated with increased expression of MEF2C and THRA2. MEF2C and THRA2 were associated with NCC markers. These findings define epigenomic features underlying lineage divergence and provide insight into NCC-related diseases such as leiomyosarcoma and neuroblastoma.
Reproduced under the paper's license (CC BY), from the paper cited above.
Code
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Data
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Data and code availability
• Data: The gene expression and DNA methylation microarray data reported in this paper have been deposited in the GEO under accession numbers GSE286162 and GSE286164. • Code: This paper does not report original code. • Other items: Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.
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Versions
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Version 1, 28 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 9 authors, 3 keywords, 3 funders, 43 references, 5 RRIDs.
Cite
This paper
Mukae, K., Shindo, M., Shi, T., Onuki, R., Yamashita, S., Hattori, N., Ushijima, T., Ohira, M., & Kamijo, T. (2026). Epigenomic regulation of neural crest differentiation in human-induced pluripotent stem cells. iScience, 29(6), 115993. https://
BibTeX
@article{mukae2026epigen
author = {Mukae, Kyosuke and Shindo, Maya and Shi, Tianyuan and Onuki, Ritsuko and Yamashita, Satoshi and Hattori, Naoko and Ushijima, Toshikazu and Ohira, Miki and Kamijo, Takehiko},
title = {{Epigenomic regulation of neural crest differentiation in human-induced pluripotent stem cells}},
journal = {iScience},
year = {2026},
month = may,
volume = {29},
number = {6},
pages = {115993},
publisher = {Elsevier},
issn = {2589-0042},
doi = {10.1016/
url = {https://
pmid = {42231962},
pmcid = {PMC13224019}
}
RIS
TY - JOUR
AU - Mukae, Kyosuke
AU - Shindo, Maya
AU - Shi, Tianyuan
AU - Onuki, Ritsuko
AU - Yamashita, Satoshi
AU - Hattori, Naoko
AU - Ushijima, Toshikazu
AU - Ohira, Miki
AU - Kamijo, Takehiko
TI - Epigenomic regulation of neural crest differentiation in human-induced pluripotent stem cells
T2 - iScience
J2 - iScience
PY - 2026
DA - 2026/
VL - 29
IS - 6
SP - 115993
SN - 2589-0042
PB - Elsevier
DO - 10.1016/
UR - https://
LA - en
ER -
CSL-JSON
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"given": "Toshikazu"
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"family": "Ohira",
"given": "Miki"
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"family": "Kamijo",
"given": "Takehiko"
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"URL": "https://
"language": "en",
"issued": {
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