OSCR

Sleep differentially modulates synaptic vesicle protein 2A and spine density across cortical regions and development.

Overview

Authors: Jing Ma1,2, Alexandra Braun1, Angela Oskamp1, Lena Kricsfalussy-Hrabár1,2, Nadja Hermes1, Salma Djakkani1, Ulrike Holz1, Sabine Jakobs1, Sabina Klein1, Stefan Stüsgen3, Philipp Krapf3, Bernd Neumaier3, Alexander Drzezga1,4,5, Simone Beer1, Andreas Bauer1,6, Astrid Rollenhagen7, Björn Kampa2,7, David Elmenhorst1,4
ORCID iDs: Jing Ma
  1. Institute of Neurosciences and Medicine, Molecular Organization of the Brain (INM-2), Forschungszentrum Jülich, Jülich, Germany
  2. Systems Neurophysiology, Institute of Zoology, RWTH Aachen University, Aachen, Germany
  3. Institute of Neuroscience and Medicine, Nuclear Chemistry (INM-5), Forschungszentrum Jülich, Jülich, Germany
  4. Department of Nuclear Medicine, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany
  5. German Center for Neurodegenerative Diseases (DZNE), Bonn-Cologne, Germany
  6. Department of Neurology, Medical Faculty, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany
  7. JARA BRAIN, Institute of Neuroscience and Medicine (INM-10), Forschungszentrum Jülich, Jülich, Germany
Institutions: Forschungszentrum Jülich (Germany); RWTH Aachen University (Germany); University of Cologne (Germany); German Center for Neurodegenerative Diseases (Germany); University Hospital Cologne (Germany); Heinrich Heine University Düsseldorf (Germany); Jülich Aachen Research Alliance (Germany)
Journal: iScience, volume 29, issue 8, article 116676
Dates: received 23 January 2026; accepted 18 June 2026; published online 15 July 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1016/j.isci.2026.116676 · PMID 42519017 · PMCID PMC13383866 · OpenAlex W7168345221
Open access: gold, a free copy (OpenAlex)
Status: code on request
Categories: histology / microscopy (modality), PET / SPECT (modality), human (organism), mouse (organism), cellular / molecular (subfield)
Methods: Statistics, Physiology & signal measures
Keywords: synaptic plasticity, SV2A, dendritic spines, sleep, sleep deprivation, PET imaging
Topic: Sleep and Wakefulness Research (Cognitive Neuroscience, Neuroscience), according to OpenAlex
Funding: Bundesministerium für Forschung Technologie und Raumfahrt Informationstechnikzentrum Bund
Citations: not cited yet (Europe PMC); 74 references in the paper

Abstract

Sleep is proposed to regulate synaptic strength; yet, the underlying mechanisms remain debated under the synaptic homeostasis hypothesis (SHY). We examined synaptic vesicle glycoprotein 2A (SV2A) and dendritic spine density in mouse brain across conditions of physiological sleep and sleep deprivation. Spine density decreased during sleep and increased following sleep deprivation, consistent with the SHY. In contrast, [18F]SynVesT-1 positron emission tomography (PET) imaging and [3H]UCB-J autoradiography revealed reduced SV2A following sleep deprivation. Layer-specific analyses in somatosensory and visual cortices further supported coordinated regulation between SV2A and dendritic spines. Developmentally, adolescent mice exhibited higher baseline SV2A and spine density than adults, with greater SV2A sensitivity to sleep deprivation, whereas adults were more responsive to physiological sleep. Together, these findings reveal bidirectional regulation of pre- and postsynaptic compartments and highlight developmental differences in sleep-dependent synaptic organization.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper says that its authors' code is available on request: it was not published with the paper, so there is nothing to verify.

The paper's code and data availability statement is in the Data section.

Tracing map

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Data

No dataset and no data link were found in the paper.

Data and code availability

All data generated or analyzed during this study are included in this published article and its supplemental information files. Additional information is available from the lead contact upon reasonable request.

This study did not generate custom code.

Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon reasonable request.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 2, 28 September 2026

  • Authors: added Jing Ma (0009-0000-1991-1515); removed Jing Ma

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 18 authors, 6 keywords, 1 funder, 74 references.

Cite

This paper

Ma, J., Braun, A., Oskamp, A., Kricsfalussy-Hrabár, L., Hermes, N., Djakkani, S., Holz, U., Jakobs, S., Klein, S., Stüsgen, S., Krapf, P., Neumaier, B., Drzezga, A., Beer, S., Bauer, A., Rollenhagen, A., Kampa, B., & Elmenhorst, D. (2026). Sleep differentially modulates synaptic vesicle protein 2A and spine density across cortical regions and development. iScience, 29(8), 116676. https://doi.org/10.1016/j.isci.2026.116676

BibTeX

@article{ma2026sleep,
author = {Ma, Jing and Braun, Alexandra and Oskamp, Angela and Kricsfalussy-Hrabár, Lena and Hermes, Nadja and Djakkani, Salma and Holz, Ulrike and Jakobs, Sabine and Klein, Sabina and Stüsgen, Stefan and Krapf, Philipp and Neumaier, Bernd and Drzezga, Alexander and Beer, Simone and Bauer, Andreas and Rollenhagen, Astrid and Kampa, Björn and Elmenhorst, David},
title = {{Sleep differentially modulates synaptic vesicle protein 2A and spine density across cortical regions and development}},
journal = {iScience},
year = {2026},
month = jul,
volume = {29},
number = {8},
pages = {116676},
publisher = {Elsevier},
issn = {2589-0042},
doi = {10.1016/j.isci.2026.116676},
url = {https://doi.org/10.1016/j.isci.2026.116676},
pmid = {42519017},
pmcid = {PMC13383866}
}

RIS

TY - JOUR
AU - Ma, Jing
AU - Braun, Alexandra
AU - Oskamp, Angela
AU - Kricsfalussy-Hrabár, Lena
AU - Hermes, Nadja
AU - Djakkani, Salma
AU - Holz, Ulrike
AU - Jakobs, Sabine
AU - Klein, Sabina
AU - Stüsgen, Stefan
AU - Krapf, Philipp
AU - Neumaier, Bernd
AU - Drzezga, Alexander
AU - Beer, Simone
AU - Bauer, Andreas
AU - Rollenhagen, Astrid
AU - Kampa, Björn
AU - Elmenhorst, David
TI - Sleep differentially modulates synaptic vesicle protein 2A and spine density across cortical regions and development
T2 - iScience
J2 - iScience
PY - 2026
DA - 2026/07/15
VL - 29
IS - 8
SP - 116676
SN - 2589-0042
PB - Elsevier
DO - 10.1016/j.isci.2026.116676
UR - https://doi.org/10.1016/j.isci.2026.116676
LA - en
ER -

CSL-JSON

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