Establishing mouse forebrain organoids as models of intrinsic cortical network assembly.
Overview
- Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
- Department of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
- Department of Molecular, Cellular and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
- Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
- Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
- Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
- Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
- Biotechnology Program, Berkeley City College, Berkeley, CA 94704, USA
- Institute of Developmental Neurophysiology, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
Abstract
The mouse cortex is a canonical model for studying how functional neural networks emerge, yet it remains unclear which topological features arise from intrinsic cellular organization versus sensory input. Mouse forebrain organoids provide a powerful system to investigate these intrinsic mechanisms. We generated dorsal (DF) and ventral (VF) forebrain organoids from mouse pluripotent stem cells and tracked their development using longitudinal electrophysiology. DF organoids showed progressively stronger network-wide correlations, while VF organoids developed more refined activity patterns with enhanced small-world topology and increased modular organization. Both organoid types form small-world networks, but their topological organization differs. These differences emerge without extrinsic inputs and correlate with Pvalb+ interneuron enrichment in VF organoids. Our findings demonstrate how cellular composition influences neural circuit self-organization, establishing mouse forebrain organoids as a tractable platform to study cortical network architecture.
Reproduced under the paper's license (CC BY), from the paper cited above.
Code
No file of the authors' code could be read here: it is described below, and read at its source.
braingeneers/Sakura_final
Availability: 1 check, the latest on 30 September 2026: the link is dead
- 30 September 2026: the link is dead
The paper's code and data availability statement is in the Data section.
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Data
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Data and code availability
The accesion numbers for the scRNAseq data reported in this paper is GEO: GSE290330, GSE312396, and the scRNAseq data is also available in the UCSC Cell Browser: https://
Reproduced under the paper's license (CC BY), from the paper cited above.
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Version 1, 30 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 20 authors, 5 keywords, 9 MeSH terms, 8 funders, 34 references, 5 RRIDs.
Cite
This paper
Hernandez, S., Schweiger, H. E., Cline, I., Kaurala, G. A., Robbins, A., Solis, D., Vera-Choqqueccota, S., Geng, J., van der Molen, T., Reyes, F., Asogwa, C. N., Voitiuk, K., Chini, M., Rolandi, M., Salama, S. R., Colquitt, B. M., Sharf, T., Haussler, D., Teodorescu, M., & Mostajo-Radji, M. A. (2026). Establishing mouse forebrain organoids as models of intrinsic cortical network assembly. Stem cell reports, 21(4), 102832. https://
BibTeX
@article{hernandez2026es
author = {Hernandez, Sebastian and Schweiger, Hunter E. and Cline, Isabel and Kaurala, Gregory A. and Robbins, Ash and Solis, Daniel and Vera-Choqqueccota, Samira and Geng, Jinghui and van der Molen, Tjitse and Reyes, Francisco and Asogwa, Chinweike Norman and Voitiuk, Kateryna and Chini, Mattia and Rolandi, Marco and Salama, Sofie R. and Colquitt, Bradley M. and Sharf, Tal and Haussler, David and Teodorescu, Mircea and Mostajo-Radji, Mohammed A.},
title = {{Establishing mouse forebrain organoids as models of intrinsic cortical network assembly}},
journal = {Stem cell reports},
year = {2026},
month = mar,
volume = {21},
number = {4},
pages = {102832},
publisher = {Elsevier},
issn = {2213-6711},
doi = {10.1016/
url = {https://
pmid = {41791384},
pmcid = {PMC13083800}
}
RIS
TY - JOUR
AU - Hernandez, Sebastian
AU - Schweiger, Hunter E.
AU - Cline, Isabel
AU - Kaurala, Gregory A.
AU - Robbins, Ash
AU - Solis, Daniel
AU - Vera-Choqqueccota, Samira
AU - Geng, Jinghui
AU - van der Molen, Tjitse
AU - Reyes, Francisco
AU - Asogwa, Chinweike Norman
AU - Voitiuk, Kateryna
AU - Chini, Mattia
AU - Rolandi, Marco
AU - Salama, Sofie R.
AU - Colquitt, Bradley M.
AU - Sharf, Tal
AU - Haussler, David
AU - Teodorescu, Mircea
AU - Mostajo-Radji, Mohammed A.
TI - Establishing mouse forebrain organoids as models of intrinsic cortical network assembly
T2 - Stem cell reports
J2 - Stem Cell Reports
PY - 2026
DA - 2026/
VL - 21
IS - 4
SP - 102832
SN - 2213-6711
PB - Elsevier
DO - 10.1016/
UR - https://
LA - en
ER -
CSL-JSON
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