OSCR

Cytoarchitecture of neurogenic niche and neuroblast clusters in the postnatal microminipig brain.

Overview

Authors: Daijiro Kojima1,2, Masato Sawada1,3, Taisei Ishimaru4, Nodoka Ito4, Shinichiro Tateyama1,2, Kazuhide Adachi2, Hiroaki Kawaguchi5,6, Noriaki Satake7, Vicente Herranz-Pérez8,9, José Manuel García-Verdugo8, Yuichi Hirose2, Nobuhiko Ohno10,11, Naoko Kaneko1,4, Kazunobu Sawamoto1,3
ORCID iDs: Kazunobu Sawamoto
  1. Department of Developmental and Regenerative Neurobiology, Institute of Brain Science, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan
  2. Department of Neurosurgery, Fujita Health University, Toyoake 470-1192, Japan
  3. Division of Neural Development and Regeneration, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
  4. Laboratory of Neuronal Regeneration, Graduate School of Brain Science, Doshisha University, Kyotanabe 610-0394, Japan
  5. Laboratory of Veterinary Pathology, Kitasato University School of Veterinary Medicine, Towada 034-8628, Japan
  6. Laboratory of Veterinary Histopathology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan
  7. Fuji Micra Inc., Fujinomiya 418-0005, Japan
  8. Laboratory of Comparative Neurobiology, Cavanilles Institute, University of Valencia, CIBERNED–ISCIII, 46980 Valencia, Spain
  9. Department of Cell Biology, Functional Biology and Physical Anthropology, University of Valencia, CIBERNED–ISCIII, 46100 Burjassot, Spain
  10. Department of Anatomy, Division of Histology and Cell Biology, Jichi Medical University, School of Medicine, Shimotsuke 329-0498, Japan
  11. Division of Ultrastructural Research, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
Institutions: Fujita Health University (Japan); Nagoya City University (Japan); National Institute for Physiological Sciences (Japan); Doshisha University (Japan); Kagoshima University (Japan); Kitasato University (Japan); Universitat de València (Spain); Jichi Medical University (Japan)
Journal: Stem cell reports, volume 21, issue 5, article 102893
Dates: received 27 October 2025; accepted 18 March 2026; published online 16 April 2026; in print May 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1016/j.stemcr.2026.102893 · PMID 41997151 · PMCID PMC13163217 · OpenAlex W7154593023
Open access: gold, a free copy (OpenAlex)
Status: code on request
Categories: human (organism), developmental (subfield)
Keywords: neural stem cells, postnatal neurogenesis, neuronal migration, microminipig, ventricular-subventricular zone
MeSH: Brain*, Neural Stem Cells*, Neurogenesis*, Neurons*, Stem Cell Niche*, Animals, Animals, Newborn, Cell Movement, Lateral Ventricles, Neuroglia, Swine, Swine, Miniature (* major topic)
Topic: Neurogenesis and neuroplasticity mechanisms (Developmental Neuroscience, Neuroscience), according to OpenAlex
Funding: Takeda Science Foundation; Mizutani Foundation for Glycoscience; Japan Agency for Medical Research and Development (25ym0126807, 24gm1210007h0006, 21bm0704033h0003); Nagoya City University (2412145, 2401101); Japan Society for the Promotion of Science (23K27270, 19H04757, 21K06395, 24K09660, JPJSCCA20230007, 23H02579, 14544620, 19H04785, 18K14823, 22H04926, JPJSBP120229939, 20K09377, 21H05106, 20H03565, 20H05700, 18KK0213); National Bioscience Database Center (JPMJFR2146); National Institute for Physiological Sciences
Citations: cited by 1 paper (Europe PMC); 38 references in the paper

Abstract

The ventricular-subventricular zone (V-SVZ) is the largest neurogenic niche in the postnatal mammalian brain, but its organization and migratory dynamics remain poorly understood in gyrencephalic species. Here, we provide ultrastructural and three-dimensional characterization of the V-SVZ neuroblasts in postnatal microminipigs, the smallest pig strain with unique advantages for experimental neuroscience. Transmission electron microscopy revealed developmental changes in cell composition and cytoarchitecture, with migratory neuroblasts consistently associated with glial cells and vasculatures. Notably, serial block-face scanning electron microscopy revealed that tier 3 neuroblasts, a gyrencephalic-specific population, formed elongated, chain-like clusters aligned along vessels, with conserved intracellular features such as polarized organelle distributions and growth cone extension. Radial glial fibers were prominent in neonates but diminished with age, suggesting a developmental shift to vascular scaffolds as primary migration guides. These findings establish microminipigs as a tractable gyrencephalic model for studying postnatal neurogenesis, offering new opportunities for translational research on brain repair.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper says that its authors' code is available on request: it was not published with the paper, so there is nothing to verify.

The paper's code and data availability statement is in the Data section.

Tracing map

A tracing map links a paper to the code its authors published: this paper has none (its code is available on request), so it has no map.

Data

No dataset and no data link were found in the paper.

Data and code availability

Any information required to reanalyze the data reported in this paper is available from the lead contact upon request.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 29 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 14 authors, 5 keywords, 12 MeSH terms, 7 funders, 38 references.

Cite

This paper

Kojima, D., Sawada, M., Ishimaru, T., Ito, N., Tateyama, S., Adachi, K., Kawaguchi, H., Satake, N., Herranz-Pérez, V., García-Verdugo, J. M., Hirose, Y., Ohno, N., Kaneko, N., & Sawamoto, K. (2026). Cytoarchitecture of neurogenic niche and neuroblast clusters in the postnatal microminipig brain. Stem cell reports, 21(5), 102893. https://doi.org/10.1016/j.stemcr.2026.102893

BibTeX

@article{kojima2026cytoarchitecture,
author = {Kojima, Daijiro and Sawada, Masato and Ishimaru, Taisei and Ito, Nodoka and Tateyama, Shinichiro and Adachi, Kazuhide and Kawaguchi, Hiroaki and Satake, Noriaki and Herranz-Pérez, Vicente and García-Verdugo, José Manuel and Hirose, Yuichi and Ohno, Nobuhiko and Kaneko, Naoko and Sawamoto, Kazunobu},
title = {{Cytoarchitecture of neurogenic niche and neuroblast clusters in the postnatal microminipig brain}},
journal = {Stem cell reports},
year = {2026},
month = apr,
volume = {21},
number = {5},
pages = {102893},
publisher = {Elsevier},
issn = {2213-6711},
doi = {10.1016/j.stemcr.2026.102893},
url = {https://doi.org/10.1016/j.stemcr.2026.102893},
pmid = {41997151},
pmcid = {PMC13163217}
}

RIS

TY - JOUR
AU - Kojima, Daijiro
AU - Sawada, Masato
AU - Ishimaru, Taisei
AU - Ito, Nodoka
AU - Tateyama, Shinichiro
AU - Adachi, Kazuhide
AU - Kawaguchi, Hiroaki
AU - Satake, Noriaki
AU - Herranz-Pérez, Vicente
AU - García-Verdugo, José Manuel
AU - Hirose, Yuichi
AU - Ohno, Nobuhiko
AU - Kaneko, Naoko
AU - Sawamoto, Kazunobu
TI - Cytoarchitecture of neurogenic niche and neuroblast clusters in the postnatal microminipig brain
T2 - Stem cell reports
J2 - Stem Cell Reports
PY - 2026
DA - 2026/04/16
VL - 21
IS - 5
SP - 102893
SN - 2213-6711
PB - Elsevier
DO - 10.1016/j.stemcr.2026.102893
UR - https://doi.org/10.1016/j.stemcr.2026.102893
LA - en
ER -

CSL-JSON

{
"id": "10.1016/j.stemcr.2026.102893",
"type": "article-journal",
"title": "Cytoarchitecture of neurogenic niche and neuroblast clusters in the postnatal microminipig brain",
"container-title": "Stem cell reports",
"author": [
{
"family": "Kojima",
"given": "Daijiro"
},
{
"family": "Sawada",
"given": "Masato"
},
{
"family": "Ishimaru",
"given": "Taisei"
},
{
"family": "Ito",
"given": "Nodoka"
},
{
"family": "Tateyama",
"given": "Shinichiro"
},
{
"family": "Adachi",
"given": "Kazuhide"
},
{
"family": "Kawaguchi",
"given": "Hiroaki"
},
{
"family": "Satake",
"given": "Noriaki"
},
{
"family": "Herranz-Pérez",
"given": "Vicente"
},
{
"family": "García-Verdugo",
"given": "José Manuel"
},
{
"family": "Hirose",
"given": "Yuichi"
},
{
"family": "Ohno",
"given": "Nobuhiko"
},
{
"family": "Kaneko",
"given": "Naoko"
},
{
"family": "Sawamoto",
"given": "Kazunobu"
}
],
"container-title-short": "Stem Cell Reports",
"volume": "21",
"issue": "5",
"page": "102893",
"DOI": "10.1016/j.stemcr.2026.102893",
"PMID": "41997151",
"PMCID": "PMC13163217",
"ISSN": "2213-6711",
"publisher": "Elsevier",
"URL": "https://doi.org/10.1016/j.stemcr.2026.102893",
"language": "en",
"issued": {
"date-parts": [
[
2026,
4,
16
]
]
}
}

Similar papers

The papers with a page that share the most with this one: the tools found in their code, their categories, datasets, cited references and authors, the rarest counting most.

[1] doi:10.1016/j.isci.2026.117318
Dynamic interactions with neuroblasts promote oligodendrocyte progenitor migration to injured cortex.
Journal: iScience
In common: 5 references
[2] doi:10.1016/j.stemcr.2026.103015 [code]
Brain injury reactivates a developmental program driving genesis and integration of transient LGE-class interneurons.
Journal: Stem cell reports
In common: 4 references
[3] doi:10.1038/s41467-026-71360-9 [code]
Perinatal brain developmental transition revealed by transcriptomic and proteomic analyses of Bama miniature pigs.
Journal: Nature communications
In common: developmental, 3 references
[4] doi:10.1038/s42003-026-10120-3
Developmental trajectories of somatostatin-positive interneurons in the human auditory cortex from migration to maturation.
Journal: Communications biology
In common: developmental, 1 reference
[5] doi:10.1038/s42003-026-10802-y
Transcriptome of fetal cortex of tree shrew underlying the emergence of outer subventricular zone.
Journal: Communications biology
In common: developmental, 1 reference
[6] doi:10.1038/s42003-026-10366-x
Molecular signatures and lineage diversification of neurogenic and gliogenic radial glia in the gyrencephalic ferret cortex.
Journal: Communications biology
In common: 1 reference
[7] doi:10.3390/biom16050621
Calretinin and Parvalbumin Trapping of TDP43 and XRCC1 Instructs Neocortical Interneuron Death in Neonatal Hypoxic-Ischemic Encephalopathy.
Journal: Biomolecules
In common: 1 reference
[8] doi:10.3390/ijms27135902
Functional Expression of Nicotinic Receptors on iPSC-Derived Astrocytes and Signalling Disturbances by a Panel of Neonicotinoid Pesticides and Their Metabolites.
Journal: International journal of molecular sciences
In common: 1 reference
[9] doi:10.1016/j.omtn.2026.103028
Intranasal Tat-modified PEG-PCL nanomicelles delivering anti-RelA siRNA attenuate ischemia-reperfusion injury.
Journal: Molecular therapy. Nucleic acids
In common: 1 reference
[10] doi:10.1038/s44318-026-00775-3
Quiescent neural stem cells transiently become neuron-like to coordinate long-range reactivation.
Journal: The EMBO journal
In common: 1 reference

Contribute

The authors of this paper can claim it, correct its record and validate its tracing map, and the maintainers of its code (its owner, or a public member of its organization) correct what it says of their repository; anyone signed in can ask for its removal. Every request goes to OSCR's own machine, which answers it; your account page follows them.

Sign in with ORCID to claim this paper as one of its authors, correct its record or validate its tracing map: when the paper's metadata lists your ORCID iD, you are recognized at once. Maintainers of its code: sign in with GitHub, then claim the repository on your account page.

Request its removal

To ask OSCR to remove this record, the copies of its authors' scripts or its tracing map, use the removal request page: signed in, you say who you are, what to remove and why, then review and confirm the request. Published rules decide every request (how).

Discussion, reproductions, activity

Discussion: questions and error reports about this paper and its code, from signed-in readers and its authors. It opens with sign-in.

Reproductions: reports from readers who ran the authors' code: what they reproduced, with which environment, commit and data. It opens with sign-in.

Activity: what happens around this paper: new versions of its record, its map's validation, discussions and reproductions. It opens with sign-in.