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Greatwall depletion from Xenopus oocytes reveals a key role of the cyclin B/CDK1-PP2A-B55 balance in the coordination of meiotic events.

Overview

Authors: Sylvain Roque1, Célia Ben Choug1, Cedric Hassen Khodja2, Suzanne Vigneron1, Véronique Legros3, Guillaume Chevreux3, Benjamin Lacroix1, Anna Castro1, Thierry Lorca1
ORCID iDs: Cedric Hassen Khodja, Guillaume Chevreux, Anna Castro, Thierry Lorca
  1. Université de Montpellier, Centre de Recherche en Biologie cellulaire de Montpellier (CRBM)-CNRS, Montpellier, France
  2. Université de Montpellier, Centre de Recherche en Biologie cellulaire de Montpellier-Montpellier Ressources Imagery (MRI) Biocampus, Montpellier, France
  3. ProteoSeine Mass Spectrometry Facility, Université Paris Cité, Institut Jacques Monod, Paris, France
Institutions: Centre National de la Recherche Scientifique (France); Université de Montpellier (France); Centre de Recherche en Biologie cellulaire de Montpellier (France); BioCampus Montpellier (France); Université Paris Cité (France); Institut Jacques Monod (France)
Journal: Nature communications, volume 17, issue 1, article 6361
Dates: received 27 July 2025; accepted 21 April 2026; published online 13 May 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1038/s41467-026-73011-5 · PMID 42129183 · PMCID PMC13376403 · OpenAlex W4414826468
Open access: gold, a free copy (OpenAlex)
Preprint: doi:10.1101/2025.10.03.680315
Status: data only
Categories: other (organism), cellular / molecular (subfield)
Methods: Statistics, Smoothing, state filtering, decompositions, Evoked potentials
Keywords: Kinases, Cell division, Cell signalling
MeSH: CDC2 Protein Kinase*, Cyclin B*, Meiosis*, Oocytes*, Protein Phosphatase 2*, Protein Serine-Threonine Kinases*, Xenopus Proteins*, Animals, Cell Cycle Proteins, Female, Phosphorylation, Protein Kinases, Protein-Tyrosine Kinases, Proto-Oncogene Proteins c-mos, Spindle Apparatus, Xenopus, Xenopus laevis (* major topic)
Topic: Microtubule and mitosis dynamics (Cell Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: Fondation ARC pour la Recherche sur le Cancer (ARC Foundation for Cancer Research) (PJA3 TL ARCPJA2023060006673); Agence Nationale de la Recherche (French National Research Agency) (ANR-22-CE13-0022, ANR-20-CE13-0033)
Citations: not cited yet (Europe PMC); 37 references in the paper

Abstract

Meiotic progression relies on maintaining a precise balance between cyclin B/CDK1 activity and the phosphatase PP2A-B55. The latter is negatively regulated by the Greatwall kinase (Gwl). In maturing Xenopus oocytes, we show that the loss of Gwl and the subsequent hyperactivation of PP2A-B55 prevents phosphorylation of key proteins involved in spindle formation, chromosome condensation and spindle migration as well as the phosphorylation of Wee1/Myt1 and the APC/C complex. As a consequence, in these oocytes bipolar spindles cannot be formed and migrate to the cortex and chromosomes are partially decondensed preventing meiotic I progression. The APC/C remains inactive impairing cyclin B3 degradation and ultimately preventing Erp1 accumulation. Finally, the c-Mos-MAPK-Rsk1/2 pathway fails to activate due to the absence of c-Mos as a consequence of its improper degradation. Overall, our findings reveal a crucial role of Gwl in the coordination and progression of meiotic divisions.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

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Data

Datasets cited

Data availability

Further information and requests for resources and reagents should be directed to and will be fulfilled by the lead contact remaining data supporting the findings of this study, supplementary information, and Source data file are provided with this paper. The immunofluorescence images generated in this study have been deposited to Zenodo (10.5281/zenodo.19201904). The raw mass spectrometry proteomics data generated in this study have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD068132 (https://www.ebi.ac.uk/pride/archive/projects/PXD068132). Source data are provided with this paper.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 28 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 9 authors, 3 keywords, 17 MeSH terms, 2 funders, 37 references.

Cite

This paper

Roque, S., Ben Choug, C., Khodja, C. H., Vigneron, S., Legros, V., Chevreux, G., Lacroix, B., Castro, A., & Lorca, T. (2026). Greatwall depletion from Xenopus oocytes reveals a key role of the cyclin B/CDK1-PP2A-B55 balance in the coordination of meiotic events. Nature communications, 17(1), 6361. https://doi.org/10.1038/s41467-026-73011-5

BibTeX

@article{roque2026greatwall,
author = {Roque, Sylvain and Ben Choug, Célia and Khodja, Cedric Hassen and Vigneron, Suzanne and Legros, Véronique and Chevreux, Guillaume and Lacroix, Benjamin and Castro, Anna and Lorca, Thierry},
title = {{Greatwall depletion from Xenopus oocytes reveals a key role of the cyclin B/CDK1-PP2A-B55 balance in the coordination of meiotic events}},
journal = {Nature communications},
year = {2026},
month = may,
volume = {17},
number = {1},
pages = {6361},
publisher = {Nature Publishing Group},
issn = {2041-1723},
doi = {10.1038/s41467-026-73011-5},
url = {https://doi.org/10.1038/s41467-026-73011-5},
pmid = {42129183},
pmcid = {PMC13376403}
}

RIS

TY - JOUR
AU - Roque, Sylvain
AU - Ben Choug, Célia
AU - Khodja, Cedric Hassen
AU - Vigneron, Suzanne
AU - Legros, Véronique
AU - Chevreux, Guillaume
AU - Lacroix, Benjamin
AU - Castro, Anna
AU - Lorca, Thierry
TI - Greatwall depletion from Xenopus oocytes reveals a key role of the cyclin B/CDK1-PP2A-B55 balance in the coordination of meiotic events
T2 - Nature communications
J2 - Nat Commun
PY - 2026
DA - 2026/05/13
VL - 17
IS - 1
SP - 6361
SN - 2041-1723
PB - Nature Publishing Group
DO - 10.1038/s41467-026-73011-5
UR - https://doi.org/10.1038/s41467-026-73011-5
LA - en
ER -

CSL-JSON

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