Heroin addiction modulates transcription factor binding in regulatory regions of the human putamen.
Overview
Abstract
The ongoing opioid crisis underscores the need to elucidate the neurobiology of addiction for improved treatments. Dynamic transcription factor (TF) binding is a key driver of substance use disorders (SUDs), yet its genome-wide patterns in human brain cell types remain poorly defined. We performed a computational re-analysis of publicly available ATAC-seq data (PRJNA561094) from post-mortem putamen samples of heroin users and matched non-users, where we profiled chromatin accessibility analyzing neuronal and glial cells separately. Genome-wide TF binding sites were identified and quantified, followed by footprinting and differential binding analyses. Co-occurrence analysis was applied to identify TF pairings associated with SUD-related genes. Neurons from heroin users exhibited 38 TFs with altered binding and footprinting, while glia showed 11 differentially bound TFs, primarily from the FOS, JUN, and ZNF families. NRF1 and KLF15 were differentially bound in both cell types. These TFs were linked to SUD-associated genes and activation of neuroinflammatory, neurohormonal, and S100 pathway genes. Co-occurrence analysis identified five TF pairs in user neurons and thirteen in non-user glia interacting with SUD-related genes and driving neuroinflammatory signaling. Overall, our findings reveal distinct alterations in TF binding accessibility and co-occurrence networks in heroin users’ neurons and glia, implicating transcriptional dysregulation in addiction-related neuroinflammation and neurohormonal activity.
Supplementary Information: The online version contains supplementary material available at 10.1038/
Reproduced under the paper's license (CC BY), from the paper cited above.
Code
No file of the authors' code could be read here: it is described below, and read at its source.
github.com.mcas.ms/gudalab/tf-regulatory-region-annotation
Availability: 1 check, the latest on 28 September 2026: the link answers (HTTP 200)
- 28 September 2026: the link answers (HTTP 200)
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Data
No dataset and no data link were found in the paper.
Data availability
The raw data for this study were obtained from the NCBI Sequence Read Archive under accession PRJNA561094, as originally reported by Egervari et al. 2020 (Nat. Commun. 11, 4634; 2020).
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 1, 28 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 3 authors, 8 keywords, 13 MeSH terms, 4 funders, 92 references.
Cite
This paper
Chakraborty, R., Veerappa, A., & Guda, C. (2026). Heroin addiction modulates transcription factor binding in regulatory regions of the human putamen. Scientific reports, 16(1), 21737. https://
BibTeX
@article{chakraborty2026
author = {Chakraborty, Rajashree and Veerappa, Avinash and Guda, Chittibabu},
title = {{Heroin addiction modulates transcription factor binding in regulatory regions of the human putamen}},
journal = {Scientific reports},
year = {2026},
month = may,
volume = {16},
number = {1},
pages = {21737},
publisher = {Nature Publishing Group},
issn = {2045-2322},
doi = {10.1038/
url = {https://
pmid = {42120535},
pmcid = {PMC13357565}
}
RIS
TY - JOUR
AU - Chakraborty, Rajashree
AU - Veerappa, Avinash
AU - Guda, Chittibabu
TI - Heroin addiction modulates transcription factor binding in regulatory regions of the human putamen
T2 - Scientific reports
J2 - Sci Rep
PY - 2026
DA - 2026/
VL - 16
IS - 1
SP - 21737
SN - 2045-2322
PB - Nature Publishing Group
DO - 10.1038/
UR - https://
LA - en
ER -
CSL-JSON
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"DOI": "10.1038/
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"ISSN": "2045-2322",
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"URL": "https://
"language": "en",
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