OSCR

Optimized nuclei isolation and snRNA-seq reveal oligodendrocyte pathway dysregulation in MOGHE brain tissue from pediatric patients.

Overview

Authors: Clara Tuccari di San Carlo1, Roberto Semeraro2, Valentina Cetica1, Dalila De Vita1, Benedetta Mazzinghi3, Antonio Falace4, Cristiana Pelorosso1, Paola Romagnani3,5, Alberto Magi2, Renzo Guerrini1,6, Valerio Conti1
  1. Department of Neuroscience and Human Genetics, Meyer Children’s Hospital IRCCS, Viale Pieraccini 24, 50139 Florence, Italy
  2. Department of Experimental and Clinical Medicine, University of Florence, 50139 Florence, Italy
  3. Nephrology and Dialysis Unit, Meyer Children’s Hospital IRCCS, 50139 Florence, Italy
  4. Pediatric Neurology and Muscular Diseases Unit, IRCCS Istituto “Giannina Gaslini”, 16147 Genoa, Italy
  5. Department of Experimental and Biomedical Sciences “Mario Serio”, University of Florence, Florence, Italy
  6. Department of Neuroscience, Pharmacology, and Child Health, University of Florence, Florence, Italy
Institutions: Meyer Children's Hospital (Italy); University of Florence (Italy); Istituto Giannina Gaslini (Italy)
Journal: Scientific reports, volume 16, issue 1, article 23190
Dates: received 2 February 2026; accepted 18 May 2026; published online 21 May 2026
Type: Research article · Language: English
License: CC BY-NC-ND
Identifiers: DOI 10.1038/s41598-026-54112-z · PMID 42168328 · PMCID PMC13396414 · OpenAlex W7161957986
Open access: gold, a free copy (OpenAlex)
Status: data only
Categories: genetics / omics (modality), human (organism), epilepsy (population), cellular / molecular (subfield)
Methods: Spectral & time-frequency, Statistics, Smoothing, state filtering, decompositions, Machine learning
Keywords: Biological techniques, Cell biology, Molecular biology, Neuroscience
MeSH: Brain*, Cell Nucleus*, Epilepsy*, Malformations of Cortical Development*, Oligodendroglia*, RNA, Small Nuclear*, Child, Female, Humans, Male, RNA-Seq, Sequence Analysis, RNA, Signal Transduction, Single-Cell Analysis, Single-Cell Gene Expression Analysis (* major topic)
Topic: Single-cell and spatial transcriptomics (Molecular Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: Italian ministry of Health (Current Research Annual Funding); Tuscany Region (DECODE-EE); Fondazione Cassa di Risparmio di Firenze (Human Brain Optical Mapping Project)
Citations: not cited yet (Europe PMC); 53 references in the paper

Abstract

The abstract is not reproduced here: the paper's license (CC BY-NC-ND) does not allow it. Read it in the paper, at the publisher or on Europe PMC.

Code

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Data

Datasets cited

Data availability statement

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Read it in the paper: doi.org/10.1038/s41598-026-54112-z.

Versions

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Version 1, 28 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 11 authors, 4 keywords, 15 MeSH terms, 3 funders, 53 references.

Cite

This paper

Tuccari di San Carlo, C., Semeraro, R., Cetica, V., De Vita, D., Mazzinghi, B., Falace, A., Pelorosso, C., Romagnani, P., Magi, A., Guerrini, R., & Conti, V. (2026). Optimized nuclei isolation and snRNA-seq reveal oligodendrocyte pathway dysregulation in MOGHE brain tissue from pediatric patients. Scientific reports, 16(1), 23190. https://doi.org/10.1038/s41598-026-54112-z

BibTeX

@article{tuccaridisancarlo2026optimized,
author = {Tuccari di San Carlo, Clara and Semeraro, Roberto and Cetica, Valentina and De Vita, Dalila and Mazzinghi, Benedetta and Falace, Antonio and Pelorosso, Cristiana and Romagnani, Paola and Magi, Alberto and Guerrini, Renzo and Conti, Valerio},
title = {{Optimized nuclei isolation and snRNA-seq reveal oligodendrocyte pathway dysregulation in MOGHE brain tissue from pediatric patients}},
journal = {Scientific reports},
year = {2026},
month = may,
volume = {16},
number = {1},
pages = {23190},
publisher = {Nature Publishing Group},
issn = {2045-2322},
doi = {10.1038/s41598-026-54112-z},
url = {https://doi.org/10.1038/s41598-026-54112-z},
pmid = {42168328},
pmcid = {PMC13396414}
}

RIS

TY - JOUR
AU - Tuccari di San Carlo, Clara
AU - Semeraro, Roberto
AU - Cetica, Valentina
AU - De Vita, Dalila
AU - Mazzinghi, Benedetta
AU - Falace, Antonio
AU - Pelorosso, Cristiana
AU - Romagnani, Paola
AU - Magi, Alberto
AU - Guerrini, Renzo
AU - Conti, Valerio
TI - Optimized nuclei isolation and snRNA-seq reveal oligodendrocyte pathway dysregulation in MOGHE brain tissue from pediatric patients
T2 - Scientific reports
J2 - Sci Rep
PY - 2026
DA - 2026/05/21
VL - 16
IS - 1
SP - 23190
SN - 2045-2322
PB - Nature Publishing Group
DO - 10.1038/s41598-026-54112-z
UR - https://doi.org/10.1038/s41598-026-54112-z
LA - en
ER -

CSL-JSON

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